公开(公告)号：US20230082473A1

公开(公告)日：2023-03-16

申请号：US17801715

申请日：2021-02-19

Applicant: KANEKA CORPORATION

Inventor： Fuminori KONOIKE ,  Kazunobu MINAKUCHI ,  Dai MURATA ,  Kenta KISHI ,  Masakatsu NISHIHACHIJO

IPC: B01J20/04 ,  C12N1/08 ,  C12N7/00 ,  C07K1/22 ,  B01D39/16 ,  B01D39/20

Abstract: The objective of the present invention is to provide a method for effectively and easily reducing an amount of a specific impurity in a liquid. The method for reducing an amount of a nucleic acid in a liquid according to the present invention is characterized in comprising the steps of contacting the liquid with a water-insoluble magnesium compound to adsorb at least a part of the nucleic acid on the water-insoluble magnesium compound. Also, the objective of the present invention is to provide an adsorbing filter useful for purifying a useful substance, such as an antibody and an antibody-like molecule, used as a purification material for effectively removing an impurity with easily maintaining the yield of the target substance due to excellent adsorption ability to a nucleic acid and low adsorption ability to an antibody, an antibody-like molecule or the like. The adsorbing filter is characterized in comprising the layer comprising a water-insoluble magnesium compound.

公开(公告)号：US20220031862A1

公开(公告)日：2022-02-03

申请号：US17299377

申请日：2019-12-10

Applicant: Flagship Pioneering Innovations VI, LLC

Inventor： Adam Barclay FISHER ,  Kimberly A. HOMAN ,  Troy Patrick HUBBARD ,  David Barry KOLESKY ,  Analise Zaunbrecher REEVES ,  Caitlin Nicole SPAULDING ,  Hok Hei TAM

IPC: A61K47/69 ,  A61K9/48 ,  C12N1/08

Abstract: The invention provides isolated achromosomal dynamic active systems (ADAS), including highly active ADAS. These ADAS provided by the invention can be obtained by a variety of means. Various associated methods of making and using these ADAS are provided.

公开(公告)号：US10889793B2

公开(公告)日：2021-01-12

申请号：US15639120

申请日：2017-06-30

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Sol M. Resnick ,  Drew D. Regitsky

IPC: C12M1/02 ,  C12M1/04 ,  C12M1/08 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12P17/02 ,  C12N1/08 ,  C12N1/10 ,  C12N1/12 ,  C12N1/20 ,  C12N13/00 ,  C02F3/32 ,  A01G33/00 ,  C12M1/00 ,  C12M1/12

Abstract: A fermenter can have at least one hollow fluid conduit disposed at least partially within a vessel. An external circumference of the hollow fluid conduit and an interior circumference of the vessel can define a downward flow path through which a multi-phase mixture including a liquid media and compressed gas substrate bubbles flows. An interior circumference of the hollow fluid conduit can defined an upward flow path which is in fluid communication with the downward flow path. The multi-phase liquid can flow through the upward flow path and exit the fermenter. Cooling may be provided in the hollow fluid conduit or the vessel. One or more backpressor generators can be used to maintain a backpressure on the fermenter. One or more fluid movers can be used to variously create an induced and/or forced flow in the downward and upward flow paths.

公开(公告)号：US10301588B2

公开(公告)日：2019-05-28

申请号：US15533432

申请日：2015-12-09

Applicant: BASF SE

Inventor： Birgit Hoff ,  Stefan Haefner ,  Weol Kyu Jeong ,  Edzard Scholten

IPC: C12N1/06 ,  C12N1/08 ,  C12P19/34 ,  C12P25/00

Abstract: The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or biotransformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45° C. to 55° C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA.

公开(公告)号：US20180327827A1

公开(公告)日：2018-11-15

申请号：US15756944

申请日：2016-08-30

Applicant: Samsung Electronics Co., Ltd. ,  SAMSUNG LIFE PUBLIC WELFARE FOUNDATION

Inventor： Kyung Yeon Han ,  Dong Hyun Park ,  Woongyang Park

IPC: C12Q1/6869 ,  C12N1/08 ,  C12N15/10 ,  C12Q1/6876 ,  C12N15/115 ,  G01N35/00

CPC classification number: C12Q1/6869 ,  C12N1/08 ,  C12N15/10 ,  C12N15/1006 ,  C12N15/115 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6876 ,  G01N35/00 ,  G01N35/0098

Abstract: A method of isolating DNA and RNA from a single cell sample effectively is provided. By the method of isolating, it is possible to isolate DNA and RNA from a single cell sample, and thus genome information and transcriptome information can be simultaneously collected and/or analyzed.

公开(公告)号：US09868946B2

公开(公告)日：2018-01-16

申请号：US14440694

申请日：2013-11-01

Applicant: GIRIHLET INC.

Inventor： Anitha Jayaprakash ,  Ravi Sachidanandam

IPC: C12N9/16 ,  C12N9/14 ,  C12N9/22 ,  C12N1/08 ,  C12N15/10

CPC classification number: C12N15/1006 ,  C12N9/16 ,  C12N15/1003 ,  C12Y301/11005

Abstract: A method for preparing circular double stranded mitochondrial DNA (mtDNA) substantially free of genomic DNA (gDNA) comprising the steps of: providing a cellular lysate free of protein and RNA contaminants, precipitating cellular debris and proteins out of said lysate and obtaining a solution comprising purified circular double stranded mitochondrial DNA (mtDNA) and genomic DNA (gDNA), incubating said solution with an amount of Hind Exonuclease V for a time and at a temperature effective to cleave non-circular DNA and obtain circular double stranded mtDNA, incubating said circular double stranded mtDNA with an amount of Ampure beads effective to bind said circular double stranded mtDNA, washing said beads with ethanol, and eluting said mtDNA from said beads, wherein said method is free of ultra-centrifugation.

公开(公告)号：US20170114318A1

公开(公告)日：2017-04-27

申请号：US15394468

申请日：2016-12-29

Applicant: SCARAB GENOMICS, LLC

Inventor： Frederick R. BLATTNER ,  Balint CSORGO ,  Gyorgy POSFAI

IPC: C12N1/08 ,  C12N9/10

CPC classification number: C12N1/08 ,  C12N9/1007 ,  C12N9/1252 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12Y201/01037

Abstract: Reduced genome bacteria with improved genetic stability are provided. Also provided are methods of producing polypeptides using the reduced genome bacteria with improved genetic stability.

公开(公告)号：US09574245B2

公开(公告)日：2017-02-21

申请号：US13750723

申请日：2013-01-25

Applicant: QVELLA CORPORATION

Inventor： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

IPC: C12Q3/00 ,  C12M1/42 ,  C12M1/00 ,  C12N1/06 ,  C12N1/08 ,  C12N13/00 ,  B01L3/00 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/143 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1833 ,  B01L2400/0694 ,  C12M35/02 ,  C12M47/06 ,  C12N1/066 ,  C12N1/08 ,  C12N13/00 ,  C12Q1/686 ,  C12Q3/00

Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

Abstract translation: 提供了用于电解裂解细胞并从细胞释放大分子的装置和方法。 提供一种微流体装置，其包括具有亚毫米级厚度的平面通道，并且在其上下表面上包括电极。 在用液体填充通道之后，使得通道在液体内包含单元，在通道电极之间施加一系列交替极性的电压脉冲，其中电压脉冲的幅度和电压脉冲的脉冲宽度对于 引起细胞的不可逆电穿孔。 该通道被配置为具有热性质，使得施加电压由于焦耳加热而导致快速升温，以从电镀细胞释放大分子。 通道还可以包括内部滤波器，用于在电处理之前捕获和集中单元。

公开(公告)号：US08744163B2

公开(公告)日：2014-06-03

申请号：US13023416

申请日：2011-02-08

Applicant: Scott Morris

Inventor： Scott Morris

IPC: G06K9/00 ,  C12M1/00 ,  G01N1/30 ,  C12N1/08

CPC classification number: G01N1/30 ,  G01N1/04 ,  G01N1/06 ,  G01N1/286 ,  G01N1/31 ,  G01N1/42 ,  G01N2001/2886

Abstract: Systems, devices, and methods for removing areas of tissue are described. A programmable laser may remove precise areas of tissue while the tissue remains substantially frozen. The laser is programmed in part by analyzing a reference image of a representative tissue section. A software program may receive digital images of test slices. Areas of interest in the image may be selected by a user. The software program can then create and send cut instructions to the programmable laser. The laser may be configured to make perforated cuts to remove the area of interest without melting the removed section.

Abstract translation: 描述了用于去除组织区域的系统，装置和方法。 可编程激光器可以在组织保持基本上冷冻的同时去除组织的精确区域。 通过分析代表性组织切片的参考图像来部分地对激光进行编程。 软件程序可以接收测试片的数字图像。 图像中的感兴趣区域可以由用户选择。 软件程序可以创建并发送剪切指令给可编程激光器。 激光器可以被配置为制造穿孔切口以去除感兴趣的区域而不熔化除去的部分。

公开(公告)号：US08669096B2

公开(公告)日：2014-03-11

申请号：US13461487

申请日：2012-05-01

Applicant: Ye Zhang ,  Honglu Wu

Inventor： Ye Zhang ,  Honglu Wu

IPC: C12N1/08 ,  B01D21/00

CPC classification number: B01L3/502738 ,  B01L2400/0478 ,  B01L2400/049 ,  B01L2400/0622

Abstract: Systems and methods for isolating samples are provided. The system comprises a first membrane and a second membrane disposed within an enclosure. First and second reservoirs can also be disposed within the enclosure and adapted to contain one or more reagents therein. A first valve can be disposed within the enclosure and in fluid communication with the first reservoir, the second reservoir, or both. The first valve can also be in fluid communication with the first or second membranes or both. The first valve can be adapted to selectively regulate the flow of the reagents from the first reservoir, through at least one of the first and second membranes, and into the second reservoir.

Abstract translation: 提供了分离样品的系统和方法。 该系统包括设置在外壳内的第一膜和第二膜。 第一和第二储存器也可以设置在外壳内并适于在其中容纳一种或多种试剂。 第一阀可以设置在外壳内并且与第一储存器，第二储存器或两者流体连通。 第一阀也可以与第一或第二膜或两者流体连通。 第一阀可以适于选择性地调节来自第一储存器的试剂的流动，通过第一和第二膜中的至少一个进入第二储存器。