公开(公告)号：US20250059496A1

公开(公告)日：2025-02-20

申请号：US18781788

申请日：2024-07-23

Applicant: Chemical Evolution Ltd.

Inventor： Dustin LILLICO ,  Trevor RANDALL ,  Alejandra ENRIQUEZ ,  Markus WEISSENBERGER

IPC: C12N1/20 ,  C12N9/42 ,  C12N15/74 ,  C12P7/06

Abstract: A genetically modified ethanologenic organism which comprises: a. an exoglucanase (cex-like) polynucleotide sequence with at least 70% sequence coverage to SEQ 1 or SEQ 68, and at least 70% sequence identity to SEQ 1 or SEQ 68; and b. a β-glucosidase 1 (bg11) polynucleotide sequence with at least 70% sequence coverage to SEQ 3 or SEQ 14, and at least 70% sequence identity to SEQ 3 or SEQ 14.

公开(公告)号：US20250057183A1

公开(公告)日：2025-02-20

申请号：US18721896

申请日：2022-12-20

Applicant: SUPERBREWED FOOD, INC.

Inventor： Kartheek ANKELLA ,  Megan JONES ,  Aharon EYAL ,  Bryan P. TRACY ,  Alon KARPOL

IPC: A23J3/20 ,  A23J3/04 ,  A23J3/08 ,  A23J3/10 ,  A23J3/22 ,  C12N1/20

Abstract: Provided is a homogeneous composition comprising protein, wherein at least 1 wt % of a total protein content is bacterial protein: and a carrier selected from the group consisting of water, oil and a combination thereof, wherein a total protein content in the composition is at least 25 wt % or wherein the composition comprises at least one animal protein and a total protein content in the composition is at least 5 wt % of the total composition. Further provided are food ingredients and food products comprising the composition.

公开(公告)号：US20250051814A1

公开(公告)日：2025-02-13

申请号：US18723065

申请日：2022-12-21

Applicant: KYOWA HAKKO BIO CO., LTD. ,  KIRIN HOLDINGS KABUSHIKI KAISHA

Inventor： Tomotoshi SUGITA ,  Sotaro SANPEI

IPC: C12P19/28 ,  C07K14/245 ,  C12N1/20 ,  C12R1/19

Abstract: The present invention relates to a microorganism having a reduced or inactivated activity of a protein according to any one of the following [1] to [3] and improved productivity of lacto-N-triose II or an oligosaccharide having a lacto-N-triose II (LNTII) skeleton as compared with a parent strain: [1] a protein consisting of the amino acid sequence represented by SEQ ID NO: 2 or 4, [2] a mutant protein having an oligosaccharide transporting activity and consisting of an amino acid sequence in which 1 to 20 amino acids are deleted, substituted, inserted, or added in the amino acid sequence represented by SEQ ID NO: 2 or 4, and [3] a homologous protein having an oligosaccharide transporting activity and consisting of an amino acid sequence having an identity of 90% or more with the amino acid sequence represented by SEQ ID NO: 2 or 4.

公开(公告)号：US20250043293A1

公开(公告)日：2025-02-06

申请号：US17311675

申请日：2019-12-06

Applicant: WILLIAM MARSH RICE UNIVERSITY

Inventor： Jeffrey J. TABOR ,  Sebastian M. CASTILLO-HAIR

IPC: C12N15/75 ,  C12N1/20 ,  C12N15/63 ,  C12R1/125

Abstract: An inducible promoter expression system based on the T7 RNA Polymerase (T7 RNAP), lactose repressor (Lad), and a chimeric T7lac promoter (PT7lac), which can be integrated as a single copy into the B. subtilis genome. In the absence of IPTG. Lad strongly represses T7RNAP and PT7lac, and expression of an exemplary ORF—here superfolder green fluorescent protein (sfGFP) reporter protein—is undetectable by flow cytometry. Addition of IPTG de-represses PT7lac, and simultaneously induces expression of T7RNAP, resulting in very high sfGFP levels.

公开(公告)号：US20250042793A1

公开(公告)日：2025-02-06

申请号：US18920837

申请日：2024-10-18

Applicant: QINGDAO UNIVERSITY OF TECHNOLOGY

Inventor： Huawei WANG ,  Rongxue ZOU ,  Zhibo YANG ,  Yanan WANG ,  Xueqin WANG ,  Yingjie SUN ,  Yujun WANG ,  Shupeng LI

IPC: C02F3/34 ,  C01G45/00 ,  C02F3/10 ,  C02F101/10 ,  C12N1/20

Abstract: A manganese carbonate-supported ferrihydrite material preparation method, in which manganese sulfate and ammonium bicarbonate are respectively dissolved to obtain a manganese sulfate solution and an ammonium bicarbonate solution, and the manganese sulfate solution is added with ferrihydrite, dropwise added with the ammonium bicarbonate solution and reacted under magnetic stirring to obtain a precipitate. The precipitate is washed with distilled water and dried in a drying oven to obtain the manganese carbonate-supported ferrihydrite material. A method for treating wastewater polluted by arsenic (As) and antibiotics by using the manganese carbonate-supported ferrihydrite material is also provided.

公开(公告)号：US20250040500A1

公开(公告)日：2025-02-06

申请号：US18787255

申请日：2024-07-29

Applicant: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College

Inventor： Jong Hyun Ham

IPC: A01H1/00 ,  C12N1/20 ,  C12R1/01

Abstract: The avirulent strain A257 of the bacterial rice pathogen Burkholderia glumae is an effective priming material to reduce or suppress major diseases in rice and other plants. Protection includes that against bacterial panicle blight, sheath blight, and narrow brown leaf spot. A mutant derivative of A257, A257ΔqsmR, significantly reduces the risk that the priming agent might potentially revert to wild-type pathogenicity.

公开(公告)号：US12215359B2

公开(公告)日：2025-02-04

申请号：US17600357

申请日：2020-04-23

Applicant: Codexis, Inc.

Inventor： Xiang Yi ,  Oscar Alvizo ,  Ravi David Garcia ,  David Entwistle ,  Charlene Ching ,  James Nicholas Riggins ,  Nandhitha Subramanian

IPC: C12N9/04 ,  C12N1/20 ,  C12N15/72

Abstract: The present application provides engineered glucose dehydrogenase polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US20250034613A1

公开(公告)日：2025-01-30

申请号：US18775012

申请日：2024-07-17

Applicant: Northwestern University

Inventor： Arthur Benjamin Prindle ,  Jonathan Xia

IPC: C12Q1/02 ,  C12N1/20 ,  C12R1/19

Abstract: Provided herein are engineered bacterial biosensors and methods of use thereof for detection of gastrointestinal disease, including inflammatory bowel disease.

公开(公告)号：US20250034579A1

公开(公告)日：2025-01-30

申请号：US18716858

申请日：2022-12-06

Applicant: Cornell University

Inventor： Chun-Jun GUO ,  Wenbing JIN ,  Tingting LI

IPC: C12N15/74 ,  C12N1/20 ,  C12N15/11

Abstract: Dysbiosis has been linked to diseases such as inflammatory bowel disease and obesity. Multi-omics studies have uncovered significant associations between microbiota genes and diseases. Many of these genes are exclusively expressed in non-model microbes such as Firmicutes/Clostridia. A pipeline for building microbial genetic manipulation systems would be a first step to manipulating these genes in vivo and causally connecting them with host diseases. The present technology relates generally to compositions and the methods of preparations thereof for genetically engineering gut-microbiota in vitro. The present technology further relates to uses of compositions in vivo.

公开(公告)号：US20250034512A1

公开(公告)日：2025-01-30

申请号：US18275380

申请日：2023-03-30

Applicant: QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES)

Inventor： Lujiang HAO ,  Yuhao JU ,  Wei WANG ,  Kai SHAN ,  Xiaofei ZHANG ,  Ruiwen CAO ,  Nan LIU ,  Gangrui ZHANG ,  Xingbao WANG

IPC: C12N1/20 ,  B01D15/36 ,  C12P19/04 ,  C12R1/01

Abstract: The present disclosure provides a Pseudoalteromonas agarivorans strain and a preparation method of an exopolysaccharide (EPS), and relates to the technical field of functional bacteria. In the present disclosure, the Pseudoalteromonas agarivorans strain HJWL2022 has a deposit number of CGMCC No. 26272. Fermentation, centrifugation, alcohol precipitation, protein removal, purification, and drying are conducted with the strain HJWL2022 to obtain a pure EPS. A proportion of a monosaccharide component in the product EPS is controlled by precisely controlling a pH value in a fermentation environment of the strain HJWL2022. Targeted control of a monosaccharide ratio is achieved to enhance a certain biological activity by adjusting fermentation conditions to change a composition of a bacterial EPS.