公开(公告)号：US20240360406A1

公开(公告)日：2024-10-31

申请号：US18687438

申请日：2022-08-31

申请人： Case Western Reserve University

发明人： Paul J. Tesar ,  Benjamin L.L. Clayton

IPC分类号： C12N5/00 ,  C12N5/079

CPC分类号： C12N5/0037 ,  C12N5/0622 ,  C12N2500/32 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/25

摘要： The invention described herein provides compositions and methods to maintain astrocytes in resting, enrichment and/or maturation, or activation states so that potential therapeutic agents treating diseases or conditions associated with reactive astrocytes can be identified.

公开(公告)号：US20240352410A1

公开(公告)日：2024-10-24

申请号：US18642539

申请日：2024-04-22

申请人： UNIVERSITY OF VIRGINIA PATENT FOUNDATION

发明人： Christopher Bruce Highley ,  Jack Whitewolf ,  M. Gregory Grewal

IPC分类号： C12N5/00 ,  B33Y10/00

CPC分类号： C12N5/0068 ,  B33Y10/00 ,  C12N2535/00 ,  C12N2537/00 ,  C12N2539/00

摘要： Provided are stable hydrogels systems with polymeric fibers and method of preparation thereof. Advantageously, the present hydrogel systems can have improved stability without the need of covalent crosslinking between the fibers. Due to their unique mechanical properties and stability, the present hydrogel systems may be useful for a wide variety of applications, including 3D printing, tissue engineering, regenerative medicine, drug delivery, and implantation.

公开(公告)号：US20240352406A1

公开(公告)日：2024-10-24

申请号：US18639518

申请日：2024-04-18

申请人： Cellum Biomedical, Inc.

发明人： Richard C. Jin

IPC分类号： C12N5/00 ,  A61K9/06 ,  A61K9/16 ,  A61K47/36 ,  C12N5/071 ,  C12N5/077

CPC分类号： C12N5/0018 ,  A61K9/06 ,  A61K9/1658 ,  A61K47/36 ,  C12N5/0625 ,  C12N5/0654 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/135 ,  C12N2501/148 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/19 ,  C12N2501/22 ,  C12N2501/2301 ,  C12N2501/2306 ,  C12N2501/2308 ,  C12N2501/25 ,  C12N2502/1142 ,  C12N2502/1323

摘要： The present invention is drawn, in part, to biocompatible compositions comprising a biocompatible polymer matrix and conditioned cell medium comprising i) a cell culture medium and ii) one or more agents synthesized by and secreted from one or more cells cultured in the cell culture medium, as well as therapeutic uses thereof, particularly in modulating bone and/or gum tissue growth.

公开(公告)号：US12123024B1

公开(公告)日：2024-10-22

申请号：US18410576

申请日：2024-01-11

申请人： Andrea D. Raymond ,  Madhavan P. Nair ,  Arti Vashist ,  Mickensone Andre

发明人： Andrea D. Raymond ,  Madhavan P. Nair ,  Arti Vashist ,  Mickensone Andre

IPC分类号： C12N5/071 ,  C12N5/00

CPC分类号： C12N5/0686 ,  C12N5/0081 ,  C12N2500/50 ,  C12N2501/998

摘要： The subject invention provides compositions and methods for isolating exosomes from, for example, biological fluid samples, cell cultures and/or cultured medium. Provided are Absolute Precipitation of Exosomes (APEX) reagents and precipitation techniques to isolate exosomes from various EV subsets, cells, dead cells, and/or cell debris. Advantageously, APEX is a rapid, effortless, inexpensive, high-recovery method to extract exosomes. This method is also faster and purer at isolating exosomes from other precipitation techniques.

公开(公告)号：US12121631B2

公开(公告)日：2024-10-22

申请号：US17527679

申请日：2021-11-16

申请人： Muffin Incorporated ,  Cook Biotech Incorporated

发明人： Michael C. Hiles ,  Chad E. Johnson ,  Neal E. Fearnot ,  Thomas Payne ,  Ronald Jankowski

IPC分类号： A61L27/50 ,  A61L27/24 ,  A61L27/36 ,  A61L27/38 ,  C12M1/00 ,  C12M1/12 ,  C12M3/00 ,  C12N5/00

CPC分类号： A61L27/50 ,  A61L27/24 ,  A61L27/3604 ,  A61L27/3633 ,  A61L27/3808 ,  A61L27/3826 ,  C12M21/08 ,  C12M23/06 ,  C12M23/34 ,  C12M25/14 ,  C12N5/0062 ,  A61L2430/02 ,  A61L2430/06 ,  A61L2430/26 ,  A61L2430/28 ,  A61L2430/30 ,  A61L2430/32 ,  A61L2430/34 ,  C12N2533/54

摘要： Described are methods, cell growth substrates, and devices that are useful in preparing cell-containing graft materials for administration to patients. Tubular passages can be defined in cell growth substrates to promote distribution of cells into the substrates. Also described are methods and devices for preparing cell-seeded graft compositions, methods and devices for preconditioning cell growth substrates prior to application of cells, and cell seeded grafts having novel substrates, and uses thereof.

公开(公告)号：US20240344028A1

公开(公告)日：2024-10-17

申请号：US18580280

申请日：2022-07-22

申请人： Mosa Meat B.V.

发明人： Rada MITIC ,  Federica CANTONI ,  Sophie Claire HUBALEK ,  Laura JACKISCH ,  Arianna MEI ,  Johanna MELKE

IPC分类号： C12N5/077 ,  A23D9/00 ,  A23L13/00 ,  C12N5/00

CPC分类号： C12N5/0653 ,  A23D9/00 ,  A23L13/00 ,  C12N5/0037 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/39

摘要： The invention provides inter alia a method for differentiating an adipogenic progenitor cell. comprising the step of:—culturing an adipogenic progenitor cell in a serum-free medium for differentiating an adipogenic progenitor cell. wherein the serum-free medium comprises:—at least one peroxisome proliferator-activated receptor gamma (PPARy) agonist:—at least one hormone selected from the group consisting of insulin and hydrocortisone:—at least one cytokine and/or growth factor selected from the group consisting of bone morphogenetic protein 4 (BMP4) and epidermal growth factor (EGF); and-ascorbic acid or a derivative thereof.

公开(公告)号：US20240344016A1

公开(公告)日：2024-10-17

申请号：US18749741

申请日：2024-06-21

申请人： DEKA Products Limited Partnership

发明人： Christopher C. Langenfeld ,  David D. B. Cannan ,  Dirk A. Van Der Merwe ,  Dean Kamen ,  Jason A. Demers ,  Frederick Morgan ,  Timothy D. Moreau ,  Brian D. Tracey ,  Matthew Ware ,  Richard J. Lanigan ,  Michael A. Baker ,  David Blumberg, JR. ,  Richard E. Andrews ,  Derek G. Kane ,  Dane C. Fawkes ,  Thomas J. Bollenbach ,  Michael C. Tilley ,  Stuart A. Jacobson ,  John F. Mannisto

IPC分类号： C12M1/36 ,  A01N1/02 ,  A61L27/38 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12M1/34 ,  C12M3/00 ,  C12N5/00

CPC分类号： C12M41/48 ,  A01N1/0247 ,  A61L27/3895 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  C12M21/08 ,  C12M23/22 ,  C12M23/38 ,  C12M25/02 ,  C12M27/18 ,  C12M29/00 ,  C12M33/00 ,  C12M37/02 ,  C12M41/12 ,  C12M41/40 ,  C12M41/46 ,  C12N5/0018 ,  C12N5/0062 ,  C12N2533/00 ,  C12N2533/50 ,  C12N2533/90

摘要： A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

公开(公告)号：US20240336891A1

公开(公告)日：2024-10-10

申请号：US18575926

申请日：2022-07-05

申请人： NISSAN CHEMICAL CORPORATION

发明人： Kohei SUZUKI ,  Yuki UEDA ,  Miya HIROI ,  Yoshiomi HIROI ,  Shiho ANNO

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/52

摘要： Objects of the present invention are to provide a base film for cell culture that enables mass production of homogeneous and high-quality cell aggregates even without the use of a biological serum, and to provide a base film-forming agent for forming the base film, and a substrate for producing cell aggregate. The present inventors provide a base film-forming agent for cell culture including a polymer, a cell adhesive substance, and a solvent, wherein the polymer contains a repeating unit derived from a monomer of formula (I) below:




wherein Ua1, Ua2, Ra1, and Ra2 are as described in the specification and the claims.

公开(公告)号：US20240335590A1

公开(公告)日：2024-10-10

申请号：US18225482

申请日：2023-07-24

申请人： 3D GLOBAL BIOTECH INC.

发明人： Keng-Liang Ou ,  Hsu-An Pan ,  En-Kai Chang

IPC分类号： A61L27/36 ,  C12N5/00

CPC分类号： A61L27/3687 ,  A61L27/3633 ,  C12N5/0075 ,  C12N2531/00 ,  C12N2533/74

摘要： A method for manufacturing an extracellular matrix composition includes providing a gel, carrying out a crosslinking treatment, carrying out a single type cell culture, carrying out a decrosslinking treatment, and carrying out an extraction treatment. The crosslinking treatment includes adding a crosslinking agent to the gel to obtain a crosslinked gel. The cell culture includes implanting cells of a singular type on the crosslinked gel and incubating the cells by adding a culture solution. The decrosslinking treatment includes adding a decrosslinking agent to the crosslinked gel to obtain a decrosslinked mixture that contains an extracellular matrix. The extraction treatment includes filtering the decrosslinked mixture to obtain the extracellular matrix composition that is in a liquid state.

公开(公告)号：US20240327800A1

公开(公告)日：2024-10-03

申请号：US18625271

申请日：2024-04-03

申请人： University of Massachusetts

发明人： Feiyu Yang ,  Narciso Pavon ,  ChangHui Pak ,  Yubing Sun

IPC分类号： C12N5/071 ,  C12M1/32 ,  C12N5/00 ,  C12N5/079

CPC分类号： C12N5/0697 ,  C12M23/12 ,  C12N5/0062 ,  C12N5/0618 ,  C12N2501/41 ,  C12N2506/00 ,  C12N2513/00 ,  C12N2533/54

摘要： The present disclosure provides a device and methods for patterning organoids. The device includes a plurality of layers vertically arranged. The plurality of layers includes a base layer, a diffusion medium disposed on the base layer, a microwell layer configured above the diffusion medium, and a partitioning layer. The microwell layer includes a lower surface, an upper surface, and one or more microwells. Each microwell defines an opening configured to extend between the microwell and the diffusion medium. The partitioning layer defines a chemical reservoir and one or more medium chambers, where the wall divides the chemical reservoir from the one or more medium chambers.