公开(公告)号：US20240358894A1

公开(公告)日：2024-10-31

申请号：US18597634

申请日：2024-03-06

申请人： CellSeed Inc. ,  TOKAI UNIVERSITY EDUCATIONAL SYSTEM

发明人： Masato SATO ,  Chikako SATO ,  Tomomi KOTOKU ,  Tomoko KAWAKE

IPC分类号： A61L27/36 ,  A61L27/38 ,  C12N5/077 ,  C12N5/0775

CPC分类号： A61L27/3612 ,  A61L27/3817 ,  A61L27/3834 ,  A61L27/3852 ,  C12N5/0655 ,  C12N5/0668 ,  A61L2430/06 ,  C12N2533/30

摘要： Provided is a cell sheet suitable for cartilage repair. The present invention provides a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the cell sheet is negative for immunostaining using an antibody against type II collagen. The present invention also provides a method for producing a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the method includes culturing cells derived from a cartilage tissue on a surface of a membrane, where a temperature-responsive polymer is immobilized on the surface, to give the cell sheet. The culturing is stopped before the cell sheet becomes positive for immunostaining using an antibody against type II collagen.

公开(公告)号：US12123017B2

公开(公告)日：2024-10-22

申请号：US16767383

申请日：2017-11-27

申请人： REGENERO S.A.

发明人： Rafael Tapia Limonchi ,  Maroun Khoury ,  Daniel Meza Rojas

IPC分类号： C12N5/00 ,  C12N5/073 ,  C12N5/074 ,  C12N5/0775 ,  C12N5/0783 ,  C12N5/0784

CPC分类号： C12N5/0043 ,  C12N5/0037 ,  C12N5/0056 ,  C12N5/0603 ,  C12N5/0637 ,  C12N5/0639 ,  C12N5/0662 ,  C12N5/0696 ,  C12N2500/22 ,  C12N2500/30

摘要： The invention relates to a method and composition for the cultivation, expansion and production of extracellular vesicles, and the preservation and/or pre-treatment of cells, wherein said composition is a medium or media supplement or a solution comprising pterostilbene or the derivatives thereof, and/or a migration inhibitory factor antagonist and optionally a bivalent cation.

公开(公告)号：US12116592B2

公开(公告)日：2024-10-15

申请号：US17385560

申请日：2021-07-26

申请人： University of Louisville Research Foundation, Inc.

发明人： Mariusz Z. Ratajczak ,  Janina Ratajczak ,  Magdalena Kucia ,  Donald Miller

IPC分类号： A61K35/28 ,  A61K31/19 ,  A61K38/09 ,  A61P43/00 ,  C12N5/0735 ,  C12N5/0775 ,  C12N15/113

CPC分类号： C12N5/0606 ,  A61K31/19 ,  A61K35/28 ,  A61K38/09 ,  A61P43/00 ,  C12N5/0665 ,  C12N15/113 ,  C12N2500/38 ,  C12N2501/15 ,  C12N2501/31

摘要： Methods for ex vivo expansion of very small embryonic like stem cells in the absence of feeder cells are provided. In some embodiments the methods include providing a plurality of VSELs; and growing the VSELs in a culture medium that includes a histone deacetylase inhibitor, luteinizing hormone, follicle-stimulating hormone, and optionally transforming growth factor beta in an amount that is sufficient to overcome quiescence of the VSELs. Also provided are feeder cell-free cell cultures, ex vivo expanded VSELs, pharmaceutical compositions that include the disclosed ex vivo expanded VSELs, methods for overcoming quiescence in VSELs, methods for re-establishing imprinting in VSELs, method for treating injuries to tissues in subjects, methods for repopulating cell types in subjects, methods for bone marrow transplantation, methods for treating radiation exposure in subjects, and methods that relate to regenerative medicine.

公开(公告)号：US20240336896A1

公开(公告)日：2024-10-10

申请号：US18700035

申请日：2022-10-14

申请人： LONZA SALES AG

发明人： Shelly A. MARTIN ,  Russell E. MCCONNELL ,  Jonathan FINN ,  Scott D. ESTES

IPC分类号： C12N5/0775 ,  A61K31/713 ,  A61K35/12 ,  A61K38/20 ,  C12N15/113

CPC分类号： C12N5/0662 ,  A61K31/713 ,  A61K35/12 ,  A61K38/2013 ,  C12N15/113 ,  C12N2310/11 ,  C12N2320/32 ,  C12N2510/02

摘要： The present disclosure provides methods of producing extracellular vesicles and methods of increasing extracellular vesicle production from producer cells, which exhibit a reduced gene and/or protein function in a cholesterol biosynthetic pathway. Also provided are cell compositions having a reduced gene and/or protein function in a cholesterol biosynthetic pathway. Reducing gene and/or protein function in a cholesterol biosynthetic pathway increases the yield and production of extracellular vesicles from the producer cells.

公开(公告)号：US20240336890A1

公开(公告)日：2024-10-10

申请号：US18578773

申请日：2022-07-19

申请人： UNIVERSITA’ DEGLI STUDI DI PAVIA ,  FONDAZIONE IRCCS POLICLINICO SAN MATTEO

发明人： Ida GENTA ,  Bice CONTI ,  Rossella DORATI ,  Marco BENAZZO ,  Silvia PISANI ,  Maria Antonietta AVANZINI

IPC分类号： C12N5/00 ,  C12N5/0775 ,  D01D5/00 ,  D01F6/62 ,  D01F6/84 ,  D01F6/92

CPC分类号： C12N5/0062 ,  C12N5/0662 ,  D01D5/0038 ,  D01F6/625 ,  D01F6/84 ,  D01F6/92 ,  C12N2513/00 ,  C12N2533/40 ,  D10B2331/041 ,  D10B2509/00

摘要： The present invention consists of a method for the production of a shape-memory tissue through the use of polymeric matrices capable of temporarily modifying their shape, following the application of an external stimulus, and at the same time capable of supporting the vehiculation of cells and/or drugs.

公开(公告)号：US12097221B2

公开(公告)日：2024-09-24

申请号：US17428590

申请日：2020-02-07

申请人： REGROW BIOSCIENCES PRIVATE LIMITED

发明人： Satyen Sanghavi ,  Vinayak Kedage

IPC分类号： A61K35/19 ,  A61K35/32 ,  A61K35/51 ,  C12N5/0775 ,  C12N5/078

CPC分类号： A61K35/19 ,  A61K35/32 ,  A61K35/51 ,  C12N5/0644 ,  C12N5/0665 ,  C12N2523/00 ,  C12N2525/00

摘要： The present disclosure discloses a method for preparing a lysate, from a combination of umbilical cord blood (UCB) and maternal blood (MB) derived platelets by obtaining platelet-rich plasma via sedimentation processes and then mixing platelet-rich plasmas so obtained. The present disclosure further discloses a combination of umbilical cord blood and maternal blood platelet lysate (UCB+MB PL), and the PL so obtained shows greater efficacy as cell and tissue culture media supplement over and above its individual components, umbilical cord blood platelet lysate (UCB PL) and maternal blood platelet lysate (MB PL) and especially over foetal bovine serum (FBS). Moreover, the method of the present disclosure provides an economic, environment friendly, ethical and efficacious method to reach a cell and tissue culture supplement with far-reaching therapeutic applications.

公开(公告)号：US20240309329A1

公开(公告)日：2024-09-19

申请号：US18607107

申请日：2024-03-15

申请人： MAM Holdings of West Florida, L.L.C.

发明人： Carl Randall Harrell

IPC分类号： C12N5/0775 ,  A61K9/50 ,  A61K45/06

CPC分类号： C12N5/0662 ,  A61K9/5068 ,  A61K45/06 ,  C12N2500/02

摘要： The present invention relates generally to methods and compositions for immunotherapy and drug delivery. In particular, the present invention relates to methods of producing exosomes from mesenchymal stem cells and, optionally, loading said exosomes with one or more bioactive substances. The exosomes may be loaded using electroporation with one or more bioactive substances such as proteins, miRNAs, and/or siRNA. In specific embodiments, the exosomes may be provided to an individual in need thereof, including where the individual in need thereof is an individual having a medical disorder such as cancer and other disorders.

公开(公告)号：US20240301357A1

公开(公告)日：2024-09-12

申请号：US18272815

申请日：2022-01-17

申请人： UNIVERSITE DE GENEVE ,  LES HOPITAUX UNIVERSITAIRES DE GENEVE

发明人： SANAE EL-HARANE ,  OLIVIER PREYNAT-SEAUVE ,  KARL-HEINZ KRAUSE ,  STÉPHANE DURUAL ,  ALI MODARRESSI ,  THOMAS BRASCHLER

IPC分类号： C12N5/0775 ,  C12N5/074 ,  C12N5/0797

CPC分类号： C12N5/0667 ,  C12N5/0623 ,  C12N5/0696 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2535/10 ,  C12N2539/00

摘要： The present invention is directed to a substrate material for the formation of cell spheroids/organoids and uses thereof. The invention further relates to methods of preparation of such a substrate material and related tools and uses thereof, methods using such a substrate material for the formation of cell spheroids and resulting spheroids and uses thereof.

公开(公告)号：US20240293470A1

公开(公告)日：2024-09-05

申请号：US18578575

申请日：2022-07-13

申请人： Arne JENSEN ,  Metin COLPAN

发明人： Arne JENSEN ,  Metin COLPAN

IPC分类号： A61K35/38 ,  C12N5/0775

CPC分类号： A61K35/38 ,  C12N5/0662 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2506/1369

摘要： The invention relates to mesenchymal stem cell derived exosomes produced by a method comprising the steps of,



performing a method of producing a clonal mesenchymal stem cell line capable of producing exosomes (MSC-derived extracellular vesicles) comprising the steps of, providing human induced pluripotent stem cells (hiPSCs),

generating therefrom an immortalized clonal cell line of mesenchymal stem cells (IMSCs),
characterizing the potential of the IMSCs to produce exosomes,


cultivating the desired IMSCs such that the IMSCs produce exosomes,
isolating the exosomes.

公开(公告)号：US20240285692A1

公开(公告)日：2024-08-29

申请号：US18567926

申请日：2022-06-07

申请人： COCYTUS LTD.

发明人： Nadir ASKENASY

IPC分类号： A61K35/28 ,  A61K38/00 ,  A61P35/00 ,  C07K14/705 ,  C12N5/0775 ,  C12N5/0789

CPC分类号： A61K35/28 ,  A61P35/00 ,  C07K14/70575 ,  C12N5/0647 ,  C12N5/0663 ,  A61K38/00 ,  C12N2510/00

摘要： Methods of producing modified cells comprising providing a sample comprising hematopoietic stem or progenitor cells (HSPC) or mesenchymal stromal cells (MSC) and adsorbing a death ligand protein to a plasma membrane of the HSPCs or MSCs are provided. Modified cells, and HSPCs and MSCs having adhered thereto an exogenous death ligand protein as well as compositions comprising those cells and their use in treating cancer are also provided.