公开(公告)号：US20230055814A1

公开(公告)日：2023-02-23

申请号：US17757576

申请日：2020-12-17

申请人： Evonik Operations GmbH

发明人： Jan Marian VON HOF ,  Stefan Julian Liebig ,  Hans Henning Wenk ,  Marrit Friederike Eckstein ,  Sunay Karacocuk ,  Maxim Yavorsky

IPC分类号： C12P19/04 ,  C12P7/6454 ,  C12N9/20

摘要： A process can be used for the enzymatic preparation of sugar esters and/or sugar alcohol esters. Mixture compositions contain the sugar esters and/or sugar alcohol esters.

公开(公告)号：US11566149B2

公开(公告)日：2023-01-31

申请号：US16258567

申请日：2019-01-26

申请人： Toyota Motor Engineering & Manufacturing North America, Inc. ,  Toyota Motor Corporation ,  Regents of the University of Minnesota

发明人： Hongfei Jia ,  Ping Wang ,  Liting Zhang ,  Andreas Buthe ,  Xueyan Zhao ,  Songtao Wu ,  Masahiko Ishii ,  Minjuan Zhang

IPC分类号： C12N9/14 ,  C12N9/20 ,  C12N9/54 ,  C12N9/96 ,  C09D5/16 ,  A61K47/60 ,  C09D7/40 ,  C12N11/06 ,  C12N11/089 ,  C09D189/00 ,  C12N9/26 ,  C08G65/333 ,  C09D5/02 ,  C09D175/16

摘要： Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

公开(公告)号：US11535875B2

公开(公告)日：2022-12-27

申请号：US16066587

申请日：2016-12-29

申请人： DSM IP Assets B.V.

发明人： Paul Mugford ,  Monika Mueller ,  Martin Schurmann

IPC分类号： C12P7/6472 ,  C12N9/20

摘要： Disclosed herein are host cells expressing a polypeptide, wherein the polypeptide hydrolyzes an ester linkage of a triacylglycerol in an oil comprising at least one long-chain polyunsaturated fatty acid, methods for using such host cells, and processes for production of a lipase using such host cells.

公开(公告)号：US20220372454A1

公开(公告)日：2022-11-24

申请号：US17816892

申请日：2022-08-02

申请人： Novozymes A/S

发明人： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC分类号： C12N9/20 ,  C11D3/386 ,  C12N1/20 ,  C12N15/52 ,  C12N1/16 ,  C12N1/14

摘要： The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/LN, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US20220333088A1

公开(公告)日：2022-10-20

申请号：US17717176

申请日：2022-04-11

申请人： Novozymes A/S

发明人： Miguel Duarte Guilherme Pereira Toscano ,  Thomas Agersten Poulsen ,  Carsten Horslev Hansen ,  Lone Baunsgaard ,  Keith Gibson

IPC分类号： C12N9/20 ,  C11D3/386

摘要： The present invention relates to variants of a parent lipase which has lipase activity and comprise one or more substitutions corresponding to G23S, D27N, A40I, F51I,L, E56R, D57N, V60E,K, K98I, N101D, R118F, G163S, T231R, N233R, Y220F, T244E, and P256T using SEQ ID NO: 2 for numbering. The present invention also relates to compositions or microcapsule compositions comprising a lipase variant of the invention and to liquid products comprising a microcapsule composition of the invention as well as the use of said microcapsule composition for stabilizing lipase variants of the invention.

公开(公告)号：US20220315520A1

公开(公告)日：2022-10-06

申请号：US17618585

申请日：2019-06-12

申请人： IOI Oleo GmbH

发明人： Dirk LOCHMANN ,  Sebastian REYER ,  Michael STEHR

IPC分类号： C07C69/716 ,  C07C67/02 ,  C12P7/62 ,  C12N9/20

摘要： The invention relates to a method for producing optionally functionalized acyl-capped (acyl-blocked) 3-hydroxybutyric acids and their salts and esters as well as to the products thus obtained and their use.

公开(公告)号：US11441134B2

公开(公告)日：2022-09-13

申请号：US17128344

申请日：2020-12-21

申请人： Novozymes A/S

发明人： Carsten Hoerslev Hansen ,  Yitong Liu ,  Steffen Kayser ,  Vibeke Skovgaard Nielsen

IPC分类号： C12N9/20 ,  C11D3/386 ,  C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20

摘要： The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/LN, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, P256A/K/N/Q/R/S/T/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:10 for position numbering or selected from H198A/D/E/F/G/I/L/N/Q/S/T/V/Y, F7H/K/R, F51A/I/L/V/Y, T143A/G/S/V, A150G/V, N200H/K/Q/R, I202G/L/V, S224C/F/H/I/L/P/Y, L227D/E/K/R, V228P, P229H/K/R, V230H/K/L/R, I255A/G/N/P/S/T/V/Y, T256A/K/N/Q/R/S/P/W, A257F/H/I/L/V/Y, L259F/Y, and W260D/E/F/H/I/L/N/Q/S/T/Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

公开(公告)号：US11434475B2

公开(公告)日：2022-09-06

申请号：US17284163

申请日：2019-10-08

申请人： Novozymes A/S

发明人： Hiroaki Udagawa

IPC分类号： C12N9/20 ,  C12N1/14 ,  C07K14/38 ,  C12N9/34

摘要： The present invention relates to filamentous fungal cells secreting a polypeptide of interest, wherein the expression of a citT gene is altered, reduced or eliminated compared to a non-mutated otherwise isogenic or parent cell, and methods of producing a secreted polypeptide of interest in said cells as well as methods of producing said cells.

公开(公告)号：US20220267783A1

公开(公告)日：2022-08-25

申请号：US17630107

申请日：2020-06-25

申请人： Novozymes A/S

发明人： Dominique Aubert Skovlund

IPC分类号： C12N15/80 ,  C12N9/20 ,  C12N9/24 ,  C12N9/88 ,  C12N9/90 ,  C12N9/00

摘要： The present invention provides recombinant filamentous fungal host cells producing one or more secreted polypeptide of interest, said cells comprising in their genome at least one nucleic acid construct comprising a first polynucleotide encoding a signal peptide operably linked in translational fusion to a second polynucleotide encoding the polypeptide of interest, wherein the first polynucleotide is heterologous to the second polynucleotide, wherein the first polynucleotide is a polynucleotide having at least 70% sequence identity with SEQ ID NO:1 or a polynucleotide encoding a signal peptide having at least 70% sequence identity with SEQ ID NO:2, as well as methods of producing one or more secreted polypeptide of interest.

公开(公告)号：US11414684B2

公开(公告)日：2022-08-16

申请号：US17157162

申请日：2021-01-25

申请人： Stichting Wageningen Research ,  Technische Universiteit Delft

发明人： Carmen Gabriela Boeriu ,  Anamaria Todea ,  Isabella Wilhelmina Christina Everdina Arends ,  Ludwina Gerharda Otten

IPC分类号： C12N9/88 ,  C12P7/6436 ,  C12P7/64 ,  C12P7/42 ,  C12N9/20 ,  C11C1/00 ,  C12P7/6418

摘要： It has been found that esterification of a hydroxy-fatty acid by a lipase can be coupled with oleate hydratase (OHase) generation of that hydroxy-FA from an unsaturated FA with a cis C9-C10 double bond, e.g. oleic acid, in a single aqueous buffered reaction medium at low temperature, e.g. 30° C. A simple one-pot enzymatic method to produce fatty acid estolides from one or more triglycerides, e.g. starting from a natural plant oil, is thereby enabled in which the same lipase catalyses both the initial hydrolysis of triglyceride and the final esterification step.