公开(公告)号：US20250066828A1

公开(公告)日：2025-02-27

申请号：US18724381

申请日：2023-01-10

Applicant: UAB BIOMATTER DESIGNS ,  ENGINZYME AB

Inventor： Martyna KOPLUNAITE ,  Nina URBELIENE ,  Rolandas MEŠKYS ,  Diana IKASALAITE ,  Linas ZAKRYS ,  Laurynas KARPUS ,  Federica RUGGIERI ,  Sascha GROBE

IPC: C12P19/38 ,  C07H19/10 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90

Abstract: Provided herein is a one-pot method of synthesising a C-nucleoside-5′-monophosphate such as pseudouridine-5′-monophosphate, from an N-nucleoside using a multi-step enzymatic pathway. The method comprises reacting the N-nucleoside with a phosphate source and nucleoside phosphorylase to form a pentose-1-phosphate intermediate, reacting the pentose-1-phosphate intermediate with a phosphomutase to form pentose-5-phosphate intermediate, and reacting the pentose-5-phosphate intermediate with a nucleoside-5′-phosphate C-glycosidase to form the C-nucleoside-5′-monophosphate.

公开(公告)号：US12234492B2

公开(公告)日：2025-02-25

申请号：US17150900

申请日：2021-01-15

Applicant: LanzaTech, Inc.

Inventor： Fungmin Liew ,  Michael Koepke ,  Shilpa Nagaraju ,  Audrey Harris

IPC: C12N9/88 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12P7/22

Abstract: Disclosed herein are improved methods for production of 2-phenylethanol by microbial fermentation of substrates comprising carbon monoxide and/or carbon dioxide and further disclosed are genetically modified microorganisms for use in such methods that alleviate dependence on natural and petrochemical processes.

公开(公告)号：US20250043316A1

公开(公告)日：2025-02-06

申请号：US18717450

申请日：2022-12-12

Applicant: ISOBIONICS B.V.

Inventor： Janani DURAIRAJ ,  Aalt-Jan VAN DIJK ,  Thamara HESSELINK ,  Martinus Julius BEEKWILDER ,  Elena MELILLO

IPC: C12P5/00 ,  C12N9/88 ,  C12N15/70 ,  C12R1/19

Abstract: The present invention concerns the field of recombinant manufacture of santalene and related products. In particular, it relates to a method for the manufacture of a composition comprising at least one santalene comprising the step of converting farnesyl pyrophosphate into at least one santalene, wherein said conversion is carried out by a polypeptide exhibiting santalene synthase activity. Moreover, the invention contemplates a composition comprising a mixture of beta-santalene and alpha-santalene with an excess of beta-santalene obtainable by the method of the invention. The invention also relates to the use of a santalene synthase polypeptide, a heterologous polynucleotide encoding it, a vector or gene construct comprising said polynucleotide, a host cell or a non-human transgenic organism comprising said gene construct or vector for the manufacture of a composition comprising at least one santalene, preferably, beta-santalene, more preferably a mixture of beta-santalene and alpha-santalene. Further, the present invention relates to a method for manufacturing a composition comprising at least one santalol, preferably beta-santalol, comprising producing a composition comprising at least one santalene by the aforementioned method of the invention and oxidising said at least one santalene, preferably, beta-santalene, into the respective alcohol in order to manufacture a composition comprising at least one santalol, preferably, beta-santalol. Yet, the invention pertains to a kit for the manufacture of a composition comprising at least one santalene comprising the aforementioned polypeptide. heterologous polynucleotide, vector or gene construct, host cell or non-human transgenic organism and to a non-human host cell or non-human transgenic organism expressing a polypeptide exhibiting santalene synthase activity from the aforementioned heterologous polynucleotide. vector or gene construct.

公开(公告)号：US12209267B2

公开(公告)日：2025-01-28

申请号：US18172594

申请日：2023-02-22

Applicant: MANUS BIO INC.

Inventor： Ryan N. Philippe ,  Ajikumar Parayil Kumaran ,  Christine Nicole S. Santos ,  Jason Donald ,  Stephen Sarria

IPC: C12P7/26 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

Abstract: The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.

公开(公告)号：US20250011822A1

公开(公告)日：2025-01-09

申请号：US18712520

申请日：2022-11-24

Applicant: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES

Inventor： Xueli ZHANG ,  Feiyu FAN ,  Yongyan XI ,  Hongtao XU

IPC: C12P7/46 ,  C07K14/39 ,  C12N9/00 ,  C12N9/04 ,  C12N9/88

Abstract: The present invention provides a genetically modified malic acid producing yeast strain, wherein the strain has or has enhanced malate transport protein activity and has or has enhanced NADPH-dependent malate dehydrogenase (EC 1.1.1.82) activity, optionally also has or has enhanced at least one of the following activities: (i) pyruvate carboxylase (EC 6.4.1.1) activity, (ii) phosphoenolpyruvate carboxykinase (EC 4.1.1.49) activity, (iii) phosphoenolpyruvate carboxylase activity, and (iv) biotin transport protein activity; and a preparation method thereof, a method for producing L-malic acid using the same, and use thereof.

公开(公告)号：US20250011750A1

公开(公告)日：2025-01-09

申请号：US18885239

申请日：2024-09-13

Applicant: CJ CheilJedang Corporation

Inventor： Yeon-Jae JANG ,  Sungjae YANG ,  Kwang Soo SHIN ,  Yee-Seul SO ,  Kwang Woo LEE

IPC: C12N9/88 ,  C12P13/06

Abstract: The present disclosure provides a variant polypeptide having an activity of aspartate 1-dicarboxylase; a microorganism including same; a composition for producing beta-alanine or beta-alanine-derived compounds, the composition comprising the microorganism; and a method for producing beta-alanine or beta-alanine-derived compounds, the method comprising a step for culturing the microorganism.

公开(公告)号：US20250002952A1

公开(公告)日：2025-01-02

申请号：US18709817

申请日：2021-11-29

Applicant: WACKER CHEMIE AG

Inventor： Rupert PFALLER ,  Thomas SCHLÖSSER ,  Günter WICH

IPC: C12P13/12 ,  C12N9/88

Abstract: Methods and processes for preparing L-cysteic acid. Where according to the process, acetyl-L-serine (OAS) is converted using at least one enzyme selected from the class of O-acetyl-L-serine sulfhydrylases (OAS sulfhydrylases, EC 4.2.99.8) in the presence of a salt of sulfurous acid. Where the OAS sulfhydrylase is CysM and the biotransformation is carried out under active pH control and the OAS concentration in the batch is at least 10 g/L.

公开(公告)号：US12163176B2

公开(公告)日：2024-12-10

申请号：US17553279

申请日：2021-12-16

Applicant: Wisconsin Alumni Research Foundation

Inventor： James L. Steele ,  Jeffrey Broadbent ,  Ekkarat Phrommao

IPC: C12P7/06 ,  C07K14/195 ,  C12N1/16 ,  C12N1/20 ,  C12N1/38 ,  C12N9/88 ,  C12P7/10 ,  C12P21/02

Abstract: An ethanologen for producing biofuel from one or more carbohydrates and reducing lactate and acetate production in a biofuel manufacturing process. The ethanologen is made by introducing into the ethanologen one or more exogenous genes required for production of a bacteriocin. The resulting ethanologen reduces lactate and acetate production by contaminant lactic acid bacteria by expression of the bacteriocin during the biofuel manufacturing process. Certain resulting ethanologens ferment sugars not naturally or not preferentially utilized by Saccharomyces cerevisiae during the manufacturing process.

公开(公告)号：US12163140B2

公开(公告)日：2024-12-10

申请号：US18495560

申请日：2023-10-26

Applicant: CERES, INC.

Inventor： Fasong Zhou ,  Kenneth A. Feldmann ,  Julissa Sosa

IPC: C12N15/82 ,  A23K10/30 ,  A23L19/00 ,  C07K14/415 ,  C12N9/88

Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.

公开(公告)号：US20240401069A1

公开(公告)日：2024-12-05

申请号：US18805564

申请日：2024-08-15

Applicant: The Regents of the University of California

Inventor： Patrick M. Shih ,  Collin Robert E. Barnum

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

Abstract: Mammalian milk oligosaccharides (MMO) are produced in plants engineered to express recombinant MMO biosynthetic pathways.