公开(公告)号：US20230139515A1

公开(公告)日：2023-05-04

申请号：US17812636

申请日：2022-07-14

申请人： Genomatica, Inc.

发明人： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Michael Kuchinskas ,  Harish Nagarajan

IPC分类号： C12N9/04 ,  C12N9/10 ,  C12P7/18 ,  C12P7/24 ,  C12P7/52 ,  C12P19/32

摘要： The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.

公开(公告)号：US20230138186A1

公开(公告)日：2023-05-04

申请号：US17979209

申请日：2022-11-02

申请人： Advanced Solutions Life Sciences, LLC

发明人： James B. Hoying ,  Hannah A. Strobel ,  Sarah Moss

IPC分类号： C12N5/071 ,  C12N5/0781 ,  C12M1/00 ,  C12M3/06 ,  C12N5/0783 ,  C12P21/00

摘要： A three-dimensional (3D) lymphoid tissue model is provided, the model including a cellularized stromal compartment and a plurality of cellularized compartments including lymphocytes disposed within the stromal compartment; and a controlled fluid perfusion system configured to perfuse the model with a perfusion fluid. Methods of fabricating a 3D lymphoid tissue model and producing antibodies with the 3D lymphoid tissue model are also provided.

公开(公告)号：US20230137836A1

公开(公告)日：2023-05-04

申请号：US17772913

申请日：2021-06-18

申请人： Seoul National University R&DB Foundation ,  Industry-Academic Cooperation Foundation, Yonsei University

发明人： Yeo-Joon Yoon ,  Myoung-Chong Song ,  Jin A Jung ,  Eunji Cheong ,  Sangwoo Kim ,  Heon Joo Lee

IPC分类号： C07D498/18 ,  A61P25/28 ,  C12N1/20 ,  C12P17/18

摘要： Provided are any one novel compound selected from the group consisting of 9-deoxo-36,37-dihydro-prolyl FK506, 9-deoxo-31-O-demethyl-36,37-dihydro-prolyl FK506, 9-deoxo-prolyl FK520, and 9-deoxo-31-O-demethyl-prolyl FK520, and use thereof.

公开(公告)号：US20230137328A1

公开(公告)日：2023-05-04

申请号：US17913096

申请日：2021-03-19

申请人： Yokogawa Electric Corporation ,  National University Corporation Shizuoka University

发明人： Makoto KAWANO ,  Hiroyuki KIMURA

IPC分类号： C12P5/02 ,  C12N1/20 ,  C12M1/107 ,  C12M1/00 ,  C12M1/06 ,  C12M1/34

摘要： A methane generating device includes: a culture tank in which methane generating bacteria are cultured with a culture liquid; a gas feed device that feeds carbon dioxide and hydrogen gas required by the methane generating bacteria to the culture tank; and a generated gas extraction device that extracts methane generated by the methane generating bacteria.

公开(公告)号：US20230137139A1

公开(公告)日：2023-05-04

申请号：US17914060

申请日：2021-03-26

申请人： Ginkgo Bioworks, Inc.

发明人： Kim Cecelia Anderson ,  Jeffrey lan Boucher ,  Elena Brevnova ,  Dylan Alexander Carlin ,  Brian Carvalho ,  Nicholas Flores ,  Katrina Forrest ,  Gabriel Rodriguez ,  Michelle Spencer

IPC分类号： C07D311/80 ,  C12N15/70 ,  C12N15/81 ,  C07C65/19 ,  C12P7/42 ,  C12P17/06 ,  C12N9/02

摘要： Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.

公开(公告)号：US20230134555A1

公开(公告)日：2023-05-04

申请号：US17911083

申请日：2021-03-05

申请人： CJ CHEILJEDANG CORPORATION

发明人： Chang Il SEO ,  Hyun Ah KIM ,  Sung Kwang SON ,  Ki Yong CHEONG ,  Moo Young JUNG ,  Tae Yeon KIM

IPC分类号： C12N9/88 ,  C12P13/22

摘要： The present disclosure relates to a method for producing L-tryptophan through the enhancement of prephenate dehydratase (PheA) activity.

公开(公告)号：US20230134060A1

公开(公告)日：2023-05-04

申请号：US18088685

申请日：2022-12-26

申请人： Locus Solutions IPCo, LLC

发明人： Sean FARMER ,  Ken ALIBEK ,  Kent ADAMS ,  Karthik N. KARATHUR

IPC分类号： C10G32/00 ,  B01D17/04 ,  C02F1/50 ,  C02F3/34 ,  C09K8/58 ,  C09K8/66 ,  C09K8/84 ,  C10G33/04 ,  C12N1/16 ,  C12P19/44 ,  E21B43/26

摘要： The subject invention provides microbe-based products, as well as their use to improve oil production and refining efficiency by, for example, remediating the disposable layers in oil tanks and other oil storage units. In preferred embodiments, the microbe-based products comprise biochemical-producing yeast and growth by-products thereof, such as, e.g., biosurfactants. The subject invention can be used to remediate rag layer and/or other dissolved solid layers that form in water-oil emulsions. Furthermore, the subject invention can be used for remediating solid impurities, such as sand, scale, rust and clay, in produced water, flow-back, brine, and/or fracking fluids.

公开(公告)号：US20230133917A1

公开(公告)日：2023-05-04

申请号：US17905859

申请日：2021-03-10

申请人： Amano Enzyme Inc.

发明人： Tatsuya SHINODA

IPC分类号： C12P7/64 ,  C12N11/00 ,  C12N9/20

摘要： The present invention addresses the problem of providing an effective new means for efficiently reducing the amount of diacylglycerol in a fat/oil (by conversion into triacylglycerol). Disclosed is a method for producing a fat/oil, including a step in which a lipase having a ratio of transesterification activity to hydrolysis activity of 0.007 or higher is caused to act on a diacylglycerol-containing fat/oil.

公开(公告)号：US20230132925A1

公开(公告)日：2023-05-04

申请号：US17453476

申请日：2021-11-03

申请人： LanzaTech, Inc.

发明人： Rachel Jane Brenc ,  Robert John Conrado ,  Joss Anton Coombes ,  Elham Ebrahimiaqda ,  Allan Haiming Gao ,  Brian Nelson Horton ,  Xueliang Li ,  Mayur Sathe ,  Curtis Paul Studebaker

IPC分类号： C12M1/00 ,  C12P7/06 ,  B01F3/04

摘要： The systems and methods disclosed herein provide for the efficient generation of fine bubbles. In particular, systems and methods for use in bioreactors are disclosed herein providing a superior means to produce useful fermentation products by the biological fermentation of fine bubble waste substrates injected into a liquid broth containing a microorganism culture.

公开(公告)号：US11639526B2

公开(公告)日：2023-05-02

申请号：US17810103

申请日：2022-06-30

申请人： GUARDANT HEALTH, INC.

发明人： AmirAli Talasaz ,  Helmy Eltoukhy ,  Stefanie Ann Ward Mortimer

IPC分类号： C12P19/34 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B15/00

摘要： Disclosed herein in are methods and systems for determining genetic variants (e.g., copy number variation) in a polynucleotide sample. A method for determining copy number variations includes tagging double-stranded polynucleotides with duplex tags, sequencing polynucleotides from the sample and estimating total number of polynucleotides mapping to selected genetic loci. The estimate of total number of polynucleotides can involve estimating the number of double-stranded polynucleotides in the original sample for which no sequence reads are generated. This number can be generated using the number of polynucleotides for which reads for both complementary strands are detected and reads for which only one of the two complementary strands is detected.