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35 条记录 (耗时 0.017 秒)

    Process for producing anticancer agent LL-D45042
    1.
    发明申请
    Process for producing anticancer agent LL-D45042 失效
    生产抗癌剂LL-D45042的方法

    公开(公告)号：US20040229323A1

    公开(公告)日：2004-11-18

    申请号：US10842277

    申请日：2004-05-10

    申请人： Wyeth Holdings Corporation

    发明人： Valerie S. Bernan ,  Edmund I. Graziani

    IPC分类号： C12P019/62 ,  C12P017/18 ,  C12N001/21

    CPC分类号： C07D498/14 ,  C12P17/188 ,  C12R1/55

    摘要： The disclosure describes the production of anticancer agent LL-D45042, having the structure: 1 by fermentation, to methods for the recovery and concentration of this anticancer agent from crude solutions, and to processes for the purification of this anticancer agent as well as a new microorganism of the species Streptomyces hygroscopicus LL-D45042 and mutants thereof useful in the preparation of this compound.

    摘要翻译： 本发明描述了具有以下结构的抗癌剂LL-D45042的制备：通过发酵，从粗溶液中回收和浓缩该抗癌剂的方法，以及该抗癌剂的纯化方法以及新的微生物 的吸水链霉菌LL-D45042及其突变体可用于制备该化合物。

    Method for producing high OD phycoerythrin
    3.
    发明申请
    Method for producing high OD phycoerythrin 审中-公开
    高OD藻红蛋白的生产方法

    公开(公告)号：US20040137583A1

    公开(公告)日：2004-07-15

    申请号：US10713029

    申请日：2003-11-17

    发明人： Young-Meng Chiang ,  Hong-Nong Chou ,  Jiunn-Ming Jeng ,  Chih-Yi Lu

    IPC分类号： C12P017/18

    CPC分类号： C12P17/182

    摘要： A new method for producing phycoerythrin with high optical density nullODnull from the group selected from an algae whose life cycle has sexual reproduction, asexual reproduction, and vegetative propagation, such as Galaxaura oblongata, Halymenia ceylanica, Helminthocladia australis, and Porphyra dentata.

    摘要翻译： 从生物周期有性繁殖，无性繁殖和营养繁殖的藻类中选择具有高光密度[藻]的藻红蛋白的新方法，例如长圆形长颈鹿，赤藓纲，南极紫菜和紫菜属。

    Production of biotin
    4.
    发明申请
    Production of biotin 审中-公开
    生物素生产

    公开(公告)号：US20030096382A1

    公开(公告)日：2003-05-22

    申请号：US09816733

    申请日：2001-03-22

    申请人： ROCHE VITAMINS INC.

    发明人： Tatsuo Hoshino ,  Akira Asakura ,  Tatsuya Kiyasu ,  Yoshie Nagahashi

    IPC分类号： C12P017/18 ,  C07D235/02

    CPC分类号： C12N9/93 ,  C12N9/13 ,  C12P17/186

    摘要： The present invention provides process for making biotin from desthiobiotin either contacting desthiobiotin with an enzyme reaction mixture containing bioB gene product and nifU gene product and/or nifS gene product and isolating the biotin or cultivating a microorganism transformed with DNA encoding the bioB gene product, nifU gene product and nifS gene product and isolating the biotin.

    摘要翻译： 本发明提供从脱硫生物素生产生物素的方法，该生物素可以将脱硫生物素与含有bioB基因产物和nifU基因产物和/或nifS基因产物的酶反应混合物接触，分离生物素或培养用编码bioB基因产物的DNA转化的微生物 基因产物和nifS基因产物并分离生物素。

    Purification of fermented clavulanic acid
    5.
    发明申请
    Purification of fermented clavulanic acid 审中-公开
    发酵的克拉维酸的纯化

    公开(公告)号：US20010029038A1

    公开(公告)日：2001-10-11

    申请号：US09871357

    申请日：2001-05-31

    发明人： Harald Summer ,  Alois Renzl ,  Helmut Wagner

    IPC分类号： C12P017/18

    CPC分类号： C07D503/00

    摘要： A process for the production of clavulanic acid and pharmaceutically acceptable salts thereof by a) fermentation of a micro-organism which is capable to produce clavulanic acid b) isolation of clavulanic acid from the fermentation broth c) purification of clavulanic acid, e.g. via a salt thereof d) conversion of the purified clavulanic acid of step c), e.g. of a salt thereof into a pharmaceutically acceptable salt of clavulanic acid, wherein the fermentation broth is extracted with a solvent prior to isolation of clavulanic acid.

    摘要翻译： 一种生产克拉维酸及其药学上可接受的盐的方法，通过以下步骤a）发酵能产生克拉维酸的微生物b）从发酵液中分离克拉维酸c）克拉维酸的纯化，例如克拉维酸的纯化。 通过其盐进行d）转化步骤c）的纯化的克拉维酸，例如 的盐转化为药学上可接受的克拉维酸盐，其中在分离棒酸之前，用溶剂萃取发酵液。

    Ansamitocins
    6.
    发明申请
    Ansamitocins 审中-公开
    安索霉素

    公开(公告)号：US20030157669A1

    公开(公告)日：2003-08-21

    申请号：US10379136

    申请日：2003-03-04

    申请人： SmithKline Beecham plc

    发明人： Mark Fulston

    IPC分类号： C12P017/18

    CPC分类号： C12P17/181 ,  C12P17/188

    摘要： Improved purification methods for ansamitocins are disclosed.

    摘要翻译： 披露了针对安曲霉素的改进的纯化方法

    Yeast with high biotin-productivity and the preparation method thereof
    8.
    发明申请
    Yeast with high biotin-productivity and the preparation method thereof 失效
    具有高生物素生产力的酵母及其制备方法

    公开(公告)号：US20030104584A1

    公开(公告)日：2003-06-05

    申请号：US09752957

    申请日：2001-01-02

    发明人： David Shiuan

    IPC分类号： C12P017/18 ,  C12N001/18 ,  C12N015/74

    CPC分类号： C12N15/52

    摘要： The invention discloses a yeast with high biotin-productivity and the preparation method thereof. The yeast is transformed by an integrated plasmid, which includes a biotin synthase gene, an assistant DNA sequence for the integration of the plasmid into a host genome, a promoter sequence, and a selection marker.

    摘要翻译： 本发明公开了一种具有高生物素生产力的酵母及其制备方法。 酵母由包含生物素合成酶基因的整合质粒转化，用于将质粒整合到宿主基因组中的辅助DNA序列，启动子序列和选择标记。

    Biosynthesis of polyketide synthase substrates
    9.
    发明申请
    Biosynthesis of polyketide synthase substrates 审中-公开
    聚酮合成酶底物的合成

    公开(公告)号：US20020192767A1

    公开(公告)日：2002-12-19

    申请号：US10087451

    申请日：2002-02-28

    发明人： Chaitan Khosla ,  Blaine Pfeifer

    IPC分类号： C12P019/62 ,  C12P017/18 ,  C12N001/21

    CPC分类号： C12N9/88 ,  C12N9/1288 ,  C12N9/93 ,  C12N15/52 ,  C12P1/00 ,  C12P11/00 ,  C12P17/08 ,  C12P17/189 ,  C12P19/62

    摘要： The use of enzymes which catalyze the production of starter and extender units for polyketides is described. In addition, modified loading modules are described, which can accept a variety of starting units such as substituted benzoates, and which can be used to generate substituted derivatives of natural products. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved in E. coli. Production can be enhanced in microbial organisms of polyketides and other secondary metabolites by delaying production until after exponential phase and by maintaining relatively constant nutrient levels in the medium. In addition, polyketide production can be enhanced by providing an expression system for a thioesterase II. Thus, by modifying the host organism and changing the culture conditions, synthesis of a secondary metabolite can be enhanced. The present invention also results in a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.

    摘要翻译： 描述了催化生产起始物的酶的使用和聚酮化合物的增量单元。 此外，描述了可以接受多种起始单元如取代的苯甲酸酯并且可用于产生天然产物的取代衍生物的改性负载模块。 这些酶可用于增强天然产生的聚酮化合物或合理设计的聚酮化合物的产率。 通过使用这些技术，已经在大肠杆菌中完成了完整的聚酮化合物的合成。 通过将生产延迟到指数期后并通过维持培养基中相对恒定的营养水平，可以增强聚酮化合物和其他次级代谢产物的微生物生物。 此外，通过提供硫酯酶II的表达系统，可以增强聚酮化合物的产生。 因此，通过改变宿主生物体并改变培养条件，可以增强次级代谢物的合成。 本发明还导致具有所需特性的宿主生物体用于生产这种聚酮化合物并评估基因改组的结果。