公开(公告)号：US11608493B2

公开(公告)日：2023-03-21

申请号：US17059821

申请日：2019-05-30

申请人： Toray Industries, Inc.

发明人： Yusuke Kagawa ,  Shingo Hiramatsu ,  Katsushige Yamada

IPC分类号： C12N9/26 ,  C12P21/04 ,  C12N1/14 ,  C12N9/42 ,  C12P19/02 ,  C12P19/14 ,  C12P21/00 ,  C07K14/37

摘要： A mutant strain of a filamentous fungus of the genus Trichoderma having a reduced function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2; and a method of producing a sugar from a cellulose-containing biomass, the method including: step a of producing a cellulase by cultivating a Trichoderma reesei mutant strain having a reduced function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2, and step b of saccharifying the biomass by using the cellulase obtained in the step a.

公开(公告)号：US11560550B2

公开(公告)日：2023-01-24

申请号：US17307255

申请日：2021-05-04

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Gang Chen ,  Darya Burakov ,  James P. Fandl

IPC分类号： C12N9/04 ,  C12P21/04 ,  C12N15/00 ,  C12P21/00 ,  C07K16/28 ,  C07K16/00

摘要： An isolated nucleic acid encoding an FX protein having a serine at position 79, a lysine at position 90, a leucine at position 136, an arginine at position 211, a serine at position 289, and a combination thereof is provided. Cells having a gene encoding a modified FX protein are provided, wherein the cells exhibit a reduced ability to fucosylate a glycoprotein at a first temperature, but exhibit the ability to fucosylate the glycoprotein at a second temperature. Methods and compositions for making glycoproteins with reduced fucosylation are provided.

公开(公告)号：US11460470B2

公开(公告)日：2022-10-04

申请号：US16783156

申请日：2020-02-05

申请人： ONE LAMBDA, INC.

发明人： Jar-How Lee ,  Neng Jen Remi Shih ,  Julie Nguyen ,  Rui Pei

IPC分类号： C12P21/04 ,  G01N33/566 ,  G01N33/564 ,  C07K14/735 ,  C07K1/00

摘要： The invention is directed to engineered Fc gamma receptor type III (FcγII, HNA-1) polypeptides and use of these polypeptides to detect antibodies specific for human neutrophil antigens (HNA). The invention is also directed to methods for the diagnosing and determining susceptibility for developing Transfusion Reaction Acute Lung (TRALI).

公开(公告)号：US11384342B2

公开(公告)日：2022-07-12

申请号：US16727212

申请日：2019-12-26

申请人： Sanofi

发明人： Bruno Dumas ,  Nabil Lounis

IPC分类号： C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04 ,  C12N15/85 ,  C12N15/65 ,  C12P21/00 ,  C12N15/09 ,  A61K38/00

摘要： The present invention is within the field of industrial protein production. The invention provides a novel expression system using dehydroorotate dehydrogenase (DHODH) as a selection marker in combination with leflunomide or a metabolite thereof, notably for use in mammalian cell lines. Expression vectors encoding DHODH, cell lines comprising said vectors and methods of producing recombinant proteins are also provided.

公开(公告)号：US11357844B2

公开(公告)日：2022-06-14

申请号：US17061891

申请日：2020-10-02

申请人： Valneva Austria GmbH ,  Valneva USA, Inc.

发明人： Larry R. Ellingsworth ,  David Flyer ,  Jing-Hui Tian ,  Steven R. Fuhrmann ,  Stefanie Kluepfel-Stahl ,  Gregory M. Glenn ,  Kerstin Westritschnig

IPC分类号： C12P21/04 ,  A61K39/08 ,  C07K14/33 ,  C07K16/12 ,  A61K39/00

摘要： This present invention provides C-TAB.G5 and C-TAB.G5.1 isolated polypeptides comprising the receptor binding domains of C. difficile toxin A and toxin B as set forth in the amino acid sequences of SEQ ID NO: 2 and SEQ ID NO: 4. The C-TAB.G5 and C-TAB.G5.1 isolated polypeptides may be used to neutralize toxic effects of C. difficile toxin A and/or toxin B.

公开(公告)号：US10696732B2

公开(公告)日：2020-06-30

申请号：US15979319

申请日：2018-05-14

申请人： Albumedix Ltd

发明人： Darrell Sleep ,  Andrew Plumridge ,  Jason Cameron ,  Inger Sandlie ,  Jan Terje Andersen ,  Esben Peter Friis

IPC分类号： A61K38/38 ,  A61K47/48 ,  C07K14/76 ,  C12P21/06 ,  C12P21/04 ,  C07K1/00 ,  C07K14/00 ,  C07K16/00 ,  C07K17/00 ,  C07H21/04 ,  C07K14/765 ,  C07K14/54 ,  A61K45/06

摘要： The present invention relates to variants of a parent albumin having altered plasma half-life compared with the parent albumin. The present invention also relates to fusion polypeptides and conjugates comprising said variant albumin.

公开(公告)号：US10683491B2

公开(公告)日：2020-06-16

申请号：US15532568

申请日：2015-12-03

申请人： NOVOZYMES A/S

发明人： Miguel Duarte Guilherme Pereira Toscano ,  Leonardo De Maria

IPC分类号： C12N9/56 ,  C12N15/00 ,  C12N1/20 ,  C12P21/06 ,  C12P21/04 ,  C11D3/386 ,  C07H21/04 ,  C12N9/54

摘要： The present invention relates to subtilase variants suitable for use in, e.g., cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.

公开(公告)号：US10544199B2

公开(公告)日：2020-01-28

申请号：US14921420

申请日：2015-10-23

申请人： Teva Pharmaceuticals Australia Pty Ltd

发明人： Collette Behrens ,  Anthony Doyle ,  Adam Clarke ,  Matthew Pollard ,  Teresa Domagala

IPC分类号： C07K14/56 ,  C07K14/00 ,  C07K16/28 ,  C12P21/04

摘要： The present invention provides a fusion polypeptide comprising a first domain and a second domain, wherein the first domain comprises a polypeptide ligand which binds to a cell surface-associated antigen and the second domain comprises aglycosylated interferon α 2b (IFNα2b) having a sequence of SEQ ID NO: 1 or SEQ ID NO: 2. The aglycosylated IFNα2b further comprises one or more amino acid substitutions or deletions which attenuate the activity of the aglycosylated IFNα2b.

公开(公告)号：US10519479B1

公开(公告)日：2019-12-31

申请号：US15457624

申请日：2017-03-13

申请人： Ares Trading S.A.

发明人： Thomas Solacroup ,  Matthieu Stettler ,  Martin Jordan ,  Hervé Broly

IPC分类号： C12N9/96 ,  C07K16/00 ,  C12P21/06 ,  C12P21/04 ,  C12P21/00 ,  C07K16/24

摘要： The present invention relates to a cell culture method for adjusting the proportion of G0, G1, G2, and high-mannose galactosylation variants in a population of a recombinant protein produced by a culture of cells in a cell culture medium by supplementing the cell culture medium with manganese at one or more days of the cell culture method duration.

公开(公告)号：US10415021B2

公开(公告)日：2019-09-17

申请号：US15521580

申请日：2015-10-23

申请人： Glycom A/S

发明人： Elise Champion ,  Andreas Vogel ,  Sebastian Bartsch ,  Gyula Dekany

IPC分类号： C12N9/26 ,  C12N15/00 ,  C12P19/18 ,  C07H21/04 ,  C12P21/06 ,  C12P21/04 ,  C12N9/10 ,  C12N9/24 ,  C12P19/04

摘要： Mutated fucosidases are provided demonstrating improved properties in terms of thermal stability and transfucosidase synthetic performance compared with a wild type transfucosidase isolated from Bifidobacterium longum subsp. infantis.