CONTAMINATION CONTROL WHEN GROWING YEASTS

    公开(公告)号:US20250066714A1

    公开(公告)日:2025-02-27

    申请号:US18532043

    申请日:2023-12-07

    Abstract: A method for contamination control when growing yeasts is provided. Bacterial contamination is controlled by using urea as the primary nitrogen source while simultaneously limiting the amount of nickel available to contaminating bacteria. Bacteria require nickel as a cofactor for urease enzymes in order to use urea for growth while yeasts do not require nickel as a cofactor for any enzymes. Nickel is limited by using titanium in plate heat exchangers instead of stainless steel. Ethyl carbamate is limited by using a carbon/nitrogen ratio that consumes all urea during fermentation and by separating co-products after fermentation and before distillation. Yeast recycling is performed by using either single-step or two-step centrifugation, without acid washing. This method enables yeast recycling with sugarcane ethanol and sugar beet ethanol production. This method also enables yeast recycling with corn ethanol and grain ethanol production with coproduct recovery after fermentation and before distillation.

    9.
    发明授权
    有权

    公开(公告)号:US11753658B2

    公开(公告)日:2023-09-12

    申请号:US17735050

    申请日:2022-05-02

    Inventor: Erin Johnson

    CPC classification number: C12P7/08 C12N1/165 C12R2001/84 Y02E50/10

    Abstract: A novel isolated Pichia stipitis strain is provided. The strain is capable of fermenting at least a pentose sugar in the presence of one or more inhibitory substances to produce ethanol. A method of utilizing the strain to produce ethanol is also provided.

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