公开(公告)号：US06750048B2

公开(公告)日：2004-06-15

申请号：US09766500

申请日：2001-01-19

申请人： Craig M. Ruecker ,  Swithin Patrick Adu-peasah ,  Brian S. Engelhardt ,  George T. Veeder, III

发明人： Craig M. Ruecker ,  Swithin Patrick Adu-peasah ,  Brian S. Engelhardt ,  George T. Veeder, III

IPC分类号： C12P764

CPC分类号： C11B3/006 ,  C11B1/02 ,  C11B3/001 ,  C12N1/06 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472 ,  C12P23/00 ,  C12P25/00 ,  C12P33/00

摘要： The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

摘要翻译： 本发明提供一种从不使用有机溶剂作为提取溶剂的微生物提取脂质的方法。 特别地，本发明提供了一种通过裂解细胞并通过用水洗涤溶液洗涤裂解的细胞混合物除去水溶性化合物和/或物质从微生物中提取脂质直至得到基本上未乳化的脂质的方法。

公开(公告)号：US06593115B2

公开(公告)日：2003-07-15

申请号：US09811808

申请日：2001-03-19

申请人： Gregory D. Vite ,  Soong-Hoon Kim ,  Gerhard Höfle

发明人： Gregory D. Vite ,  Soong-Hoon Kim ,  Gerhard Höfle

IPC分类号： C12P764

CPC分类号： C12P17/14 ,  C07D277/30

摘要： The present invention relates to a process for the preparation of intermediates useful in the synthesis of epothilone analogs by initially enzymatically degrading certain epothilone compounds to form ring-open structures containing a carboxyl group which is esterified, the hydroxyl groups on the moiety protected and the resulting compound oxidized by, e.g. ozone, to form a first intermediate. The first intermediate can be reacted with a triphenylphosphine adduct to yield a compound containing an ester group at position 1 which is subsequently hydrolyzed to form a second intermediate.

公开(公告)号：US06492146B1

公开(公告)日：2002-12-10

申请号：US09550819

申请日：2000-04-18

申请人： Lorenzo De Ferra ,  Pietro Massardo

发明人： Lorenzo De Ferra ,  Pietro Massardo

IPC分类号： C12P764

CPC分类号： C12P13/06

摘要： A process for the preparation of phosphatidylserines, comprising the reaction of phosphatides, such as phosphatidylcholine and phosphatidylethanolamine, with racemic or enantiomerically pure Serine, preferably with (L)-Serine, wherein said reaction is carried out in an aqueous dispersion of said phosphatides in the presence of a phospholipase D (PLD) and of calcium salts.

摘要翻译： 一种制备磷脂酰丝氨酸的方法，其包括磷脂（例如磷脂酰胆碱和磷脂酰乙醇胺）与外消旋或对映体纯的丝氨酸（优选与（L） - 丝氨酸）反应，其中所述反应在所述磷脂的水分散体中进行 存在磷脂酶D（PLD）和钙盐。

公开(公告)号：US06274357B1

公开(公告)日：2001-08-14

申请号：US09202583

申请日：1998-12-17

申请人： Christine Davies ,  Alasdair Robin Macrae

发明人： Christine Davies ,  Alasdair Robin Macrae

IPC分类号： C12P764

CPC分类号： C12P7/6436 ,  C12P7/20 ,  C12P7/62 ,  C12P7/6454 ,  C12P7/6472 ,  C12P19/44

摘要： Aliphatic, saturated or unsaturated, straight or branched chain C2-C24 monocarboxylic acids and polyhydric alcohols, particularly glycerol are selectively reached to monoesters in the presence of an enzyme which comprises potato lipid acyl hydrolase. The process can be used too for upgrading technical monoglycerides which contain free fatty acids.

摘要翻译： 在包含马铃薯脂质酰基水解酶的酶的存在下，脂族，饱和或不饱和的直链或支链C 2 -C 24单羧酸和多元醇，特别是甘油选择性地达到单酯。 该方法也可用于升级含有游离脂肪酸的技术性单甘油酯。

公开(公告)号：US06716610B2

公开(公告)日：2004-04-06

申请号：US09453078

申请日：1999-12-02

申请人： Masami Shimizu ,  Toshiteru Komatsu ,  Masao Shimizu ,  Minoru Kase

发明人： Masami Shimizu ,  Toshiteru Komatsu ,  Masao Shimizu ,  Minoru Kase

IPC分类号： C12P764

CPC分类号： C12P7/6418 ,  C12N11/08 ,  C12P7/6454

摘要： A lipolytic enzyme such as lipase is adsorbed on a porous anion-exchange resin carrier to obtain an immobilized enzyme without drying. The un-dried immobilized enzyme is contacted with a fat or oil for a time which may be about 2 hours to about 24 hours to stabilize the immobilized enzyme, and the immobilized enzyme is recovered from the fat or oil. The recovered immobilized enzyme is then used for esterifying or hydrolyzing a substrate. Water content of the un-dried immobilized enzyme may be 20% or more be weight such as 20-60%. Prior to immobilization, the resin carrier may be treated with a lypophylic aliphatic acid which preferably has 8 to 18 carbons.

摘要翻译： 将脂肪酶等脂肪分解酶吸附在多孔阴离子交换树脂载体上，得到不干燥的固定化酶。 将未干燥的固定化酶与脂肪或油接触约2小时至约24小时以稳定固定化酶的时间，并且从脂肪或油中回收固定化酶。 然后将回收的固定化酶用于酯化或水解底物。 未干燥固定化酶的含水量可以为20〜20重量％的20重量％以上。 在固定之前，树脂载体可以用优选具有8至18个碳原子的脂环族脂族酸处理。

公开(公告)号：US06391598B1

公开(公告)日：2002-05-21

申请号：US09538852

申请日：2000-03-28

申请人： L. Davis Clements ,  Ajoy Y. K. Koppolu

发明人： L. Davis Clements ,  Ajoy Y. K. Koppolu

IPC分类号： C12P764

CPC分类号： C07C51/412

摘要： A method for producing at least one metal salt of a fatty acid is disclosed. The process first obtains from animal rumen, ruminal fluid containing at least one fatty acid. Next, at least one metal oxide is added to the ruminal fluid. One metal salt of a fatty acid is formed from the addition of these two components. Lastly, the process involves recovering at least some of the at least one metal salt of a fatty acid. The ruminal fluid also contains enzymes which are isolated in the process.

摘要翻译： 公开了一种生产至少一种脂肪酸金属盐的方法。 该过程首先从动物瘤胃获得含有至少一种脂肪酸的瘤胃液。 接下来，向瘤胃液中加入至少一种金属氧化物。 脂肪酸的一种金属盐由这两种组分的添加形成。 最后，该方法包括回收至少一种脂肪酸的至少一种金属盐。 瘤胃液还含有在该过程中分离的酶。

公开(公告)号：US06184009B2

公开(公告)日：2001-02-06

申请号：US09068154

申请日：1998-09-30

申请人： Frederick William Cain ,  Stephen Raymond Moore ,  Gerald Patrick McNeill ,  Olga Cornelia Zwemmer

发明人： Frederick William Cain ,  Stephen Raymond Moore ,  Gerald Patrick McNeill ,  Olga Cornelia Zwemmer

IPC分类号： C12P764

CPC分类号： C12P7/6427 ,  A23D9/00 ,  A23L33/115 ,  C12P7/6436 ,  C12P7/6472 ,  C12P7/6481 ,  C12P7/649 ,  Y02E50/13 ,  Y10S426/807

摘要： A process for preparing a material B comprising geometrical isomers L1 and L2 of conjugated linoleic acid moieties in a specific weight ratio of XB wherein a material A selected from free fatty acids, mono-, di- or tri-glycerides, phospholipids, alkyl esters or wax-esters containing at least 5 weight % of L1 and L2 are subjected to enzymatic conversion using an enzyme that can discriminate between L1 and L2 so that the original weight ratio of L1 to L2 of XA in the starting material is increased to XB where XB is equal to or greater than 1.1 XA. Advantageously, the isomers L1 and L2 are cis9, trans11 and trans10, cis12 linoleic acid or vice versa. The enzyme is advantageously a lipase obtained from Geotrichum candidum or Candida rugosa or a phospholipase. The starting material A may be, for example, a fish oil or vegetable oil. The resulting products may be blended with a complementary fat and may be used as foods or food supplements or in pharmaceutical compositions.

摘要翻译： 一种制备材料B的方法，其包含XB的特定重量比的共轭亚油酸部分的几何异构体L1和L2，其中选自游离脂肪酸，单 - ，二 - 或三 - 甘油酯，磷脂，烷基酯或 含有至少5重量％的L1和L2的蜡酯使用可以区分L1和L2的酶进行酶转化，使得起始原料中XA的初始重量比L1至L2增加到XB，其中XB 等于或大于1.1 XA。 有利地，异构体L1和L2是顺式9，反式11和反式10，顺式12亚油酸或反之亦然。 所述酶有利地是从地高赤霉素或假丝酵母或磷脂酶获得的脂肪酶。 原料A可以是例如鱼油或植物油。 所得产品可与互补脂肪混合，并可用作食品或食品添加剂或药物组合物。

公开(公告)号：US06171830B2

公开(公告)日：2001-01-09

申请号：US08894363

申请日：1997-11-06

申请人： Jan Adrianus Verschoor

发明人： Jan Adrianus Verschoor

IPC分类号： C12P764

CPC分类号： C12P7/6409 ,  A61K39/04 ,  C07K16/1289 ,  C12P7/6436 ,  C12P7/6463 ,  C12P7/649 ,  G01N33/5695 ,  Y02E50/13

摘要： A method of purifying mycolic acids, salts thereof or derivatives thereof. The method involves a) providing a mixture including the mycolic acids, salts thereof or derivatives thereof and contaminants; b) dissolving the mixture in a bi-phasic solvent to form a dissolved mixture including the mycolic acids, salts thereof or derivatives thereof and contaminants; c) purifying the mycolic acids, salts thereof or derivatives thereof by subjecting the dissolved mixture to countercurrent distribution separation involving a sufficient number of cycles to separate the mycolic acids, salts thereof or derivatives thereof from the contaminants; and d) removing the separated, purified mycolic acids, salts thereof or derivatives thereof from the bi-phasic solvent. Residual impurities may be extracted from the removed mycolic acids, salts thereof or derivatives thereof with acetone.

摘要翻译： 一种纯化霉菌酸，其盐或其衍生物的方法。 该方法包括：a）提供包括霉菌酸，其盐或其衍生物和污染物的混合物; b）将混合物溶解在双相溶剂中以形成溶解的混合物，包括霉酚酸，其盐或其衍生物和污染物; c）通过使溶解的混合物经受足够数量的循环以将霉酚酸，其盐或其衍生物与污染物分离的逆流分布分离来纯化霉菌酸，其盐或其衍生物; 和d）从双相溶剂中除去分离的，纯化的霉菌酸，其盐或其衍生物。 残余杂质可以从去除的霉菌酸，其盐或其衍生物与丙酮中提取。

公开(公告)号：US06812009B2

公开(公告)日：2004-11-02

申请号：US10082340

申请日：2002-02-26

申请人： Raymond M. Gladue ,  Paul W. Behrens

发明人： Raymond M. Gladue ,  Paul W. Behrens

IPC分类号： C12P764

CPC分类号： A23D7/0053 ,  A23D9/013 ,  A23D9/05 ,  A23K10/16 ,  A23K20/10 ,  A23K20/142 ,  A23K20/158 ,  A23K20/174 ,  A23K40/10 ,  A23K50/80 ,  C11B1/10 ,  C12P7/6472 ,  C12P7/6481 ,  G02B2027/0159 ,  Y10S426/801

摘要： This invention provides a particulate material suitable for use as a nutritional supplement, particularly as an aquaculture feed. The particulate material has a high proportion of DHA residues in the lipid fraction, which may be up to 35% of the material, or even more. Preferably, the material has a mean particle size of from about 5 microns to about 10 microns. This invention also provides a method for preparing a particulate material suitable for use as an aquaculture feed from microbial biomass, preferably from algal cells having a high content of DHA residues, by obtaining a lipid fraction from the biomass, preferably by solvent extraction of broken cells, followed by separating a fraction containing phospholipids and proteins from the lipid fraction, and removing water from the protein/phospholipid fraction to form a low moisture particulate, preferably by spray-drying the protein/phospholipid fraction.

摘要翻译： 本发明提供了适合用作营养补充剂的颗粒材料，特别是作为水产养殖饲料。 颗粒材料在脂质部分中具有高比例的DHA残基，其可以高达材料的35％，甚至更多。 优选地，该材料具有约5微米至约10微米的平均粒度。 本发明还提供了一种制备适于从微生物生物质（优选来自具有高DHA残留量的藻类细胞）的水产养殖饲料制备的颗粒材料的方法，通过从生物质中获得脂质部分，优选通过溶解萃取破碎的细胞 然后从脂质部分中分离含有磷脂和蛋白质的部分，并且从蛋白质/磷脂部分除去水以形成低水分颗粒，优选通过喷雾干燥蛋白质/磷脂部分。

公开(公告)号：US06746857B2

公开(公告)日：2004-06-08

申请号：US09331759

申请日：1999-06-25

申请人： Kenichi Higashiyama ,  Toshiaki Yaguchi ,  Kengo Akimoto ,  Sakayu Shimizu

发明人： Kenichi Higashiyama ,  Toshiaki Yaguchi ,  Kengo Akimoto ,  Sakayu Shimizu

IPC分类号： C12P764

CPC分类号： C12P7/6427 ,  C12N1/14 ,  C12P7/6472

摘要： A process for controlling the mycelial morphology of a microorganism belonging to the genus Mortierella during culturing and a process for producing unsaturated fatty acids and a lipid containing them by using a culture medium for culturing a microorganism in which phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the culture medium are in the range of 5 to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively, characterized in that the microorganism belonging to the genus Mortierella is cultured in a culture medium containing phosphate ions in the range of 5 to 60 mM, potassium ions in the range of 5 to 60 mM, sodium ions in the range of 2 to 50 mM, magnesium ions in the range of 0.5 to 9 mM, and calcium ions in the range of 0.5 to 12 mM, respectively, to produce unsaturated fatty acids and the lipid containing them, and the culture medium for culturing a microorganism having phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the range of 5 to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively.

摘要翻译： 用于在培养期间控制属于被孢霉属属的微生物的菌丝体形态的方法以及通过使用培养基培养不饱和脂肪酸和含有它们的脂质的方法来培养微生物的方法，其中磷酸根离子，钾离子，钠离子， 培养基中的镁离子和钙离子分别为5〜60mM，5〜60mM，2〜50mM，0.5〜9mM，以及0.5〜12mM的范围，其特征在于，属于 将被孢霉属属菌体培养在含有5-60mM磷酸根离子，5-60mM范围钾离子，2-50mM范围内的钠离子，0.5- 至9mM，钙离子分别为0.5-12mM，以产生不饱和脂肪酸和含有它们的脂质，以及用于培养具有磷酸根离子，钾离子，钠离子，镁离子的微生物的培养基 s和钙离子分别在5至60mM，5至60mM，2至50mM，0.5至9mM和0.5至12mM的范围内。