公开(公告)号：US20250051845A1

公开(公告)日：2025-02-13

申请号：US18794611

申请日：2024-08-05

Applicant: IMMAGINA BIOTECHNOLOGY S.R.L.

Inventor： Alessia DEL PIANO ,  Tea KECMAN ,  Massimiliano CLAMER

IPC: C12Q1/6883 ,  C12N15/10 ,  C12Q1/25 ,  C12Q1/6851

Abstract: An in vitro or ex vivo method of diagnosis, prognosis, therapy monitoring or outcome prediction assessment of a disease or condition in a subject by detecting and quantifying at least one molecular marker of the disease or condition contained in a biological sample of the subject, wherein the at least one molecular marker is an RNA fragment comprising a 3′ phosphate or a 2′/3′ cyclic phosphate and kit for diagnosing Spinal Muscular Atrophy and cutaneous Squamous Cell Carcinoma.

公开(公告)号：US20240336700A1

公开(公告)日：2024-10-10

申请号：US18752283

申请日：2024-06-24

Applicant: UNIVERSITY MEDICAL CENTER HAMBURG-EPPENDORF

Inventor： Friedrich NOLTE ,  Peter BANNAS ,  Kerstin SCHÜTZE ,  William FUMEY ,  Levin SCHRIEWER ,  Stephan MENZEL ,  Catelijne STORTELERS

IPC: C07K16/28 ,  A61K39/00 ,  A61P37/06 ,  C12N15/86 ,  C12Q1/25 ,  G01N33/53

CPC classification number: C07K16/2896 ,  A61P37/06 ,  C12N15/86 ,  C12Q1/25 ,  G01N33/53 ,  A61K2039/505 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/64 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/75 ,  C07K2317/76 ,  C07K2317/92

Abstract: Methods of targeting a CD38-expressing cell for treatment of at least one disease, disorder or condition that is associated with CD38. Polypeptides specifically binding to CD38 which are therefore suitable for the diagnosis and for the therapeutic and prophylactic treatment of diseases which are characterized by increased CD38 expression. Conjugates and pharmaceutical compositions comprising the polypeptides are disclosed as well. Use of such polypeptides in methods for the detection of CD38 and/or CD38-expressing cells in a biological sample. A process for the purification and concentration of CD38 and/or CD38-expressing cells in which the antigen-binding polypeptides are used are also described.

公开(公告)号：US20240271202A1

公开(公告)日：2024-08-15

申请号：US18428519

申请日：2024-01-31

Applicant: New York University

Inventor： Gilad Evrony

IPC: C12Q1/6869 ,  C12Q1/25 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12Q1/25 ,  C12Q1/6806 ,  G01N2333/9015

Abstract: Provided are compositions and methods for improved accuracy during DNA sequencing. The method can be performed without amplification prior to sequencing. The compositions and methods are used for determining single and double stranded sequences, and for accurately determining mosaic double strand mutations and single strand nucleotide changes.

公开(公告)号：US20230408520A1

公开(公告)日：2023-12-21

申请号：US18459107

申请日：2023-08-31

Applicant: FUJIKURA KASEI CO., LTD ,  NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITY

Inventor： Rika NAKAMURA ,  Hideyuki Kuroda ,  Go Tomiyoshi ,  Takaki Hiwasa ,  Masaki Takiguchi ,  Naokatsu Saeki

IPC: G01N33/573 ,  C12Q1/25 ,  C12Q1/68 ,  G01N33/53

CPC classification number: G01N33/573 ,  C12Q1/25 ,  C12Q1/68 ,  G01N33/53

Abstract: A method for detecting anti-deoxyhypusine synthase autoantibody in a subject, wherein the method includes detecting, in a blood, serum, or plasma sample from a test subject with digestive system cancer, the amount of anti-deoxyhypusine synthase autoantibody, wherein the autoantibody is able to bind to a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1.

公开(公告)号：US11795507B2

公开(公告)日：2023-10-24

申请号：US17896520

申请日：2022-08-26

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Layla Katiraee

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

CPC classification number: C12Q1/6876 ,  C12Q1/25 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US11782012B2

公开(公告)日：2023-10-10

申请号：US18061128

申请日：2022-12-02

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Wei Zhang ,  Brian Holman ,  Kevin Horan

IPC: G01N27/333 ,  G01N33/84 ,  C12Q1/25 ,  G01N27/416 ,  G01N33/50 ,  G01N33/49 ,  G01N27/30

CPC classification number: G01N27/333 ,  C12Q1/25 ,  G01N27/301 ,  G01N27/4163 ,  G01N33/4915 ,  G01N33/50 ,  G01N33/84

Abstract: A fluid analyzer for analyzing fluid samples comprising one or more analytes and a method of calibrating such. The fluid analyzer includes a control system to control at least one automated valve to pass at least three calibration reagents through a fluid channel to a secondary ion selective electrode, a primary ion selective electrode, and a reference electrode, and determine calibration information using calibration logic from signals generated by a meter, control the at least one automated valve to selectively pass different subsets of the at least three calibration reagents through the fluid channel to the secondary ion selective electrode, the primary ion selective electrode, and the reference electrode, and determine re-calibration information using the signals generated by the meter and at least one of the calibration information and re-calibration logic.

公开(公告)号：US11754499B2

公开(公告)日：2023-09-12

申请号：US16780508

申请日：2020-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: B01L3/00 ,  G01N21/64 ,  B01J19/00 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58 ,  B01L7/00

CPC classification number: G01N21/6445 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  B01J19/0046 ,  B01L3/502761 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/573 ,  G01N33/582 ,  B01J2219/0059 ,  B01J2219/0065 ,  B01J2219/0072 ,  B01J2219/0074 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00743 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N21/6428 ,  G01N2500/00 ,  C12Q1/6827 ,  C12Q2537/161 ,  C12Q2563/125 ,  C12Q2563/131 ,  C12Q2563/159 ,  C12Q1/6804 ,  C12Q2537/161 ,  C12Q2563/125 ,  C12Q2563/159 ,  C12Q1/6851 ,  C12Q2563/159 ,  C12Q2561/113 ,  C12Q2565/1015

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.

公开(公告)号：US11718855B2

公开(公告)日：2023-08-08

申请号：US17370598

申请日：2021-07-08

Applicant: Monsanto Technology LLC

Inventor： Anisha Akula ,  David R. Duncan ,  Brenda A. Lowe ,  Michael T. Mann ,  William L. Petersen ,  Jyoti R. Rout ,  David D. Songstad ,  Joel B. Wilks ,  Wanggen Zhang

IPC: C12N15/82 ,  A01H4/00 ,  A01H1/04 ,  A01H6/46 ,  C12Q1/686 ,  C12Q1/6813 ,  C12Q1/25 ,  G01N33/53

CPC classification number: C12N15/8201 ,  A01H1/04 ,  A01H4/001 ,  A01H6/4684 ,  C12N15/821 ,  C12N15/8209 ,  C12N15/8212 ,  C12N15/8265 ,  C12N15/8205 ,  C12N15/8261 ,  C12Q1/25 ,  C12Q1/686 ,  C12Q1/6813 ,  G01N33/53

Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

公开(公告)号：US11709146B2

公开(公告)日：2023-07-25

申请号：US16701682

申请日：2019-12-03

Applicant: Case Western Reserve University

Inventor： Miklos Gratzl ,  Tamas Cseefalvi ,  Christian Zorman ,  John W. Stanton ,  Zhehao Zhang

IPC: G01N27/30 ,  G01N33/573 ,  G01N33/84 ,  G01N33/543 ,  C12Q1/25

CPC classification number: G01N27/302 ,  C12Q1/25 ,  G01N33/5438 ,  G01N33/573 ,  G01N33/84

Abstract: A microfluidic pH-stat with a specially-is designed slide and portable device can be used for point-of-care enzyme diagnostics. The slide includes a microchamber and a substrate for the enzyme being tested. The substrate is homogenized with the sample in the microchamber to form a test volume. The microchamber includes a working microelectrode that injects current to split water in the test volume to generate hydrogen ions and/or hydroxide ions and a micro-pH-electrode to measure a pH of the test volume; the slide also includes a reference microelectrode. The device includes a processor to adjust the injected current based on the pH of the test volume and determine an activity of the enzyme based on an amount the injected current is adjusted.

公开(公告)号：US11698332B2

公开(公告)日：2023-07-11

申请号：US15748393

申请日：2015-11-24

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Robert Moline ,  Manish Giri ,  Chantelle Domingue

IPC: G01N15/06 ,  B01L3/00 ,  C12Q1/25 ,  G01N33/487 ,  G01N33/543 ,  G01N1/38 ,  G01N33/50 ,  G01N15/00

CPC classification number: G01N15/0656 ,  B01L3/502715 ,  C12Q1/25 ,  G01N1/38 ,  G01N33/48707 ,  G01N33/50 ,  G01N33/54366 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0825 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0442 ,  B01L2400/0487 ,  G01N2015/0065

Abstract: Examples herein provide a device. The device includes a sample delivery component, which includes: a reagent chamber to contain at least one reagent; a sample chamber to contain a fluid sample; and a delivery channel extending from the reagent chamber and in fluid communication with the sample chamber and an output port, wherein the delivery channel is conducive mixing the at least one reagent and the fluid sample to form a mixture before the mixture reaches the output port and be discharged therefrom. The device includes a testing cassette detachable from the delivery component, which includes: an input port in fluid communication with a microfluidic reservoir, the input port to receive the discharged fluid sample from the output port; and a micro-fabricated integrated sensor in a microfluidic channel extending from the microfluidic reservoir.