公开(公告)号：US12196759B2

公开(公告)日：2025-01-14

申请号：US17723284

申请日：2022-04-18

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/58 ,  C12Q1/44 ,  C12Q1/66 ,  G01N33/50

Abstract: Described herein are methods for assessing cytotoxicity of an agent. The methods include providing a target cell that has been engineered to express intracellularly a reporter that is not expressed endogenously by the target cell, exposing the target cell to an agent capable of modulating cytotoxicity and assaying the activity of the reporter, wherein a change in reporter activity relative to a reference value is indicative of the agent being able to modulate cytotoxicity of the target cell.

公开(公告)号：US12194080B2

公开(公告)日：2025-01-14

申请号：US15770648

申请日：2016-10-28

Applicant: Dana-Farber Cancer Institute, Inc.

Inventor： Bruce M. Spiegelman ,  Jonathan Z. Long

IPC: A61K38/50 ,  A61P3/10 ,  C12N9/80 ,  C12Q1/44 ,  G01N33/50

Abstract: The present invention relates to methods for identifying, assessing, preventing, and treating metabolic disorders and modulating metabolic processes using PM20D1 and N-lipidated amino acids.

公开(公告)号：US20250003981A1

公开(公告)日：2025-01-02

申请号：US18706278

申请日：2022-12-05

Applicant: The Feinstein Institutes for Medical Research

Inventor： Christine Metz ,  Peter K. Gregersen

IPC: G01N33/68 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6883 ,  G01N1/40 ,  G01N15/10 ,  G01N15/14

Abstract: A method of non-invasively diagnosing endometriosis in a subject using single cell isolation methods and kits which separate somatic cells and epithelial tissues, with an additional step of disaggregating epithelial tissues after separation single somatic cells from a menstrual effluent sample, determining uterine NK cells, T-cells, and/or B-cells, along with diagnosing and treating dysmenorrhea.

公开(公告)号：US12181461B2

公开(公告)日：2024-12-31

申请号：US17421333

申请日：2020-01-07

Applicant: 1866402 ONTARIO LIMITED

Inventor： Jeffrey Sutton

IPC: B01L3/00 ,  C12Q1/44 ,  C12Q1/50 ,  G01N1/38 ,  G01N1/40 ,  G01N15/06 ,  G01N33/50 ,  G01N33/70 ,  G01N15/01 ,  G01N15/075

Abstract: There is provided a device and method for separation of blood, including sedimentation of plasma using PVA. The device comprises an inner container enclosed in an outer container, wherein upon alignment of respective openings, allows sample to exit from the inner container into a reaction structure. The reaction structure comprises one or more layers, each with one or more portions each containing concentrations of one or more chemicals.

公开(公告)号：US20240425902A1

公开(公告)日：2024-12-26

申请号：US18707251

申请日：2022-11-03

Applicant: Moderna TX, Inc.

Inventor： Kevin Smith

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/44

Abstract: Provided herein are methods of purifying nucleic acids (e.g., DNA) for gene synthesis using combinations of nucleases. Also provided are improved products for use in the production of RNA.

公开(公告)号：US20240417712A1

公开(公告)日：2024-12-19

申请号：US18741521

申请日：2024-06-12

Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY

Inventor： Hyongbum KIM ,  Ji-Hye PARK ,  Hanahrae LEE

IPC: C12N9/78 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44

Abstract: Please enter in the present application the English translation of the abstract of the Korean language application that was filed in the USPTO on Jun. 12, 2024, as contained in the English translation of the application in Appendix B of the Translator Certificate filed herewith, and as separately filed herewith in .docx format (728_Patent_Application_ABSTRACT.docx).

公开(公告)号：US20240309423A1

公开(公告)日：2024-09-19

申请号：US18595944

申请日：2024-03-05

Applicant: Illumina, Inc.

Inventor： Jonathan Boutell ,  Katharina Mueller-Ott ,  Jason Betley ,  Xiaolin Wu ,  Wayne George ,  Pietro Gatti Lafranconi ,  Andrew Brown

IPC: C12Q1/44 ,  C12Q1/6806

CPC classification number: C12Q1/44 ,  C12Q1/6806

Abstract: Automated methods conducted in a sequencing flowcell, and kits for reusing a flowcell, are provided herein. In some examples, an automated method conducted in a sequencing flowcell may include, at a surface of the sequencing flowcell coupled to a first moiety, using a reagent to decouple a first complex from the first moiety. In some examples, the first complex may include a second moiety which couples to the first moiety and a polynucleotide coupled to the second moiety. In some examples, the method may further include using a nuclease to polynucleotides in the sequencing flowcell. The method may include, after using the reagent and after using the nuclease, coupling a second complex to the first moiety. The second complex may include a third moiety which couples with the first moiety and an oligonucleotide coupled to the third moiety.

公开(公告)号：US12060602B2

公开(公告)日：2024-08-13

申请号：US18406159

申请日：2024-01-07

Applicant: VedaBio, Inc.

Inventor： Phillip Belgrader

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/64

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/111 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/6486 ,  C12N2310/20

Abstract: The present disclosure relates to multiplex assay methods used to detect target nucleic acids of interest from several to many sources in a sample without amplification of the target nucleic acids of interest. The methods involve partitioning of first ribonucleoprotein complexes to detect short sequences from several to many loci scattered throughout a single source genome, and the addition of signal boosting second ribonucleoprotein complexes and reporter moieties.

公开(公告)号：US20240240238A1

公开(公告)日：2024-07-18

申请号：US18406159

申请日：2024-01-07

Applicant: VedaBio, Inc.

Inventor： Phillip Belgrader

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/64

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/111 ,  C12Q1/44 ,  C12Q1/6806 ,  G01N21/6486 ,  C12N2310/20

Abstract: The present disclosure relates to multiplex assay methods used to detect target nucleic acids of interest from several to many sources in a sample without amplification of the target nucleic acids of interest. The methods involve partitioning of first ribonucleoprotein complexes to detect short sequences from several to many loci scattered throughout a single source genome, and the addition of signal boosting second ribonucleoprotein complexes and reporter moieties.

公开(公告)号：US20240182951A1

公开(公告)日：2024-06-06

申请号：US18553167

申请日：2022-03-30

Applicant: Jumpcode Genomics, Inc.

Inventor： Keith BROWN ,  Jon ARMSTRONG ,  Azeem SIDDIQUE

IPC: C12Q1/6816 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886

CPC classification number: C12Q1/6816 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886

Abstract: Disclosed herein are compositions and methods related to the detection and/or elimination of a first nucleic acid and enrichment of a second nucleic acid in a sample, for example to exclude the first nucleic acid from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.