公开(公告)号：US20240301488A1

公开(公告)日：2024-09-12

申请号：US18605197

申请日：2024-03-14

申请人： 10x Genomics, Inc.

发明人： Marlon Stoeckius

IPC分类号： C12Q1/6874 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6876

CPC分类号： C12Q1/6874 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Y207/07007 ,  C12Y301/26004 ,  C12Y605/01001 ,  G01N2333/9015 ,  G01N2333/9126 ,  G01N2333/922

摘要： Provided herein are methods of identifying a location of an RNA in a sample that include: (a) contacting the sample with an array comprising capture probes, where a capture probe comprises a capture domain and a spatial barcode; (b) releasing the RNA from the sample; (c) extending a 3′ end of the capture probe using the capture domain-bound RNA as a template; (d) generating nick(s) in the extended capture probe-hybridized RNA and performing random-primed DNA synthesis; (e) performing end repair on the second strand DNA molecule; (f) adding a single adenosine nucleotide to the 3′ end of the extended capture probe; (g) ligating a double-stranded sequencing adaptor to the double-stranded DNA product; and (h) determining all or a part of the sequence of the RNA, and the sequence of the spatial barcode, or complements thereof, and using the determined sequences to identify the location of the RNA in the sample.

公开(公告)号：US20240271204A1

公开(公告)日：2024-08-15

申请号：US18562762

申请日：2022-05-20

申请人： PEKING UNIVERSITY

发明人： Chengqi YI ,  Zhixin LEI ,  Haowei MENG ,  Zhicong LYU

IPC分类号： C12Q1/6869 ,  C12Q1/44 ,  C12Q1/48

CPC分类号： C12Q1/6869 ,  C12Q1/44 ,  C12Q1/485

摘要： Provided are a method for detecting nucleic acid sites edited by a base editor, and a kit for implementing the method. Also provided is a method for detecting the editing efficiency or off-target effects of the base editor editing nucleic acids.

公开(公告)号：US20240240266A1

公开(公告)日：2024-07-18

申请号：US18562206

申请日：2021-05-18

申请人： BioSeeds Corporation

发明人： Manish BIYANI

IPC分类号： C12Q1/70 ,  C12Q1/48 ,  C12Q1/6865 ,  G01N27/327 ,  G01N33/543 ,  G01N33/58

CPC分类号： C12Q1/70 ,  C12Q1/485 ,  C12Q1/6865 ,  G01N27/3277 ,  G01N33/543 ,  G01N33/58 ,  G01N2333/91255 ,  G01N2333/9128

摘要： An isothermal gene amplification method that allows an RNA virus to be detected easily and inexpensively in a short time is presented. The isothermal gene amplification method involves a target sequence in an RNA genome, and includes: (A) producing an amplification reaction template; (B) DNA amplifying for increasing the amplification reaction template; and (C) RNA amplifying.

公开(公告)号：US12036212B2

公开(公告)日：2024-07-16

申请号：US17705798

申请日：2022-03-28

申请人： Memorial Sloan-Kettering Cancer Center

发明人： Richard Kolesnick ,  Adriana Haimovitz-Friedman ,  Evis Sala ,  Zvi Fuks

IPC分类号： A61K31/4439 ,  A61K31/337 ,  A61K31/404 ,  A61K31/7048 ,  A61K31/7068 ,  A61K45/06 ,  A61P35/00 ,  C12Q1/48 ,  G01N33/574

CPC分类号： A61K31/4439 ,  A61K31/337 ,  A61K31/404 ,  A61K31/7048 ,  A61K31/7068 ,  A61K45/06 ,  A61P35/00 ,  C12Q1/485 ,  G01N33/57488 ,  G01N2800/52 ,  A61K31/337 ,  A61K2300/00 ,  A61K31/7068 ,  A61K2300/00 ,  A61K31/7048 ,  A61K2300/00 ,  A61K33/24 ,  A61K2300/00 ,  A61K31/4439 ,  A61K2300/00 ,  A61K31/404 ,  A61K2300/00

摘要： Disclosed is a method for enhancing tumor response to chemotherapy, the method comprising administering a short-acting anti-angiogenic agent (AAA) capable of activating ASMase to a subject afflicted with a solid tumor, and thereby creating a time interval of increased susceptibility of said tumor to one or more chemotherapeutic agents, followed by administration of at least one chemotherapeutic agent within the interval. The interval can be defined in terms of a short-duration activation of ASMase signaling by the AAA. Disclosed are also methods for predicting the tumor response in a patient afflicted with a solid tumor to a chemotherapeutic agent, using as an indicator of the response ASMase level or activity (or ceramide level) in the patient following the administration of the chemotherapeutic agent to the patient, or dynamic IVIM based DW-MRI to measure perfusion alterations following administration of the chemotherapeutic agent.

公开(公告)号：US20240228552A9

公开(公告)日：2024-07-11

申请号：US18482052

申请日：2023-10-06

申请人： UNIVERSITY OF MASSACHUSETTS

发明人： Min Chen ,  Fanjun Li

IPC分类号： C07K14/245 ,  B82Y5/00 ,  C12N9/12 ,  C12Q1/48

CPC分类号： C07K14/245 ,  B82Y5/00 ,  C12N9/12 ,  C12Q1/485 ,  C12Y207/10001

摘要： Disclosed herein are nanopore tweezer systems that can be used to screen for allosteric inhibitors of protein kinases. In some embodiments, the protein kinase is a mutant kinase that confers resistance to chemotherapeutic drugs during cancer treatment. Therefore, the disclosed systems and methods can be used to identify drugs for treating drug resistant cancers.

公开(公告)号：US20240218443A1

公开(公告)日：2024-07-04

申请号：US18393291

申请日：2023-12-21

申请人： Illumina, Inc.

发明人： Angelica Mariani ,  Antoine Francais ,  Frederick James Topping ,  Christopher Winnard ,  Philip Balding ,  Chol Steven Yun ,  Patrizia Iavicoli ,  Kathryn Hattingh ,  Xiaolin Wu ,  Xiaohai Liu

IPC分类号： C12Q1/6874 ,  B01J23/44 ,  C12Q1/48 ,  C12Q1/6806 ,  G01N21/64

CPC分类号： C12Q1/6874 ,  B01J23/44 ,  C12Q1/485 ,  C12Q1/6806 ,  G01N21/6486 ,  G01N2333/9126

摘要： The present application relates to palladium compositions, methods for sequencing by synthesis using nucleotides with 3′ blocking groups, and sequencing kits, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.

公开(公告)号：US12018299B2

公开(公告)日：2024-06-25

申请号：US16956435

申请日：2018-12-20

申请人： SEVIVO LLC

发明人： Donald R. Ronning

IPC分类号： C12N9/24 ,  C12Q1/34 ,  C12Q1/48

CPC分类号： C12N9/2497 ,  C12Q1/34 ,  C12Q1/48 ,  C12Y302/02016 ,  C12N2320/10 ,  G01N2333/91011

摘要： Methods and kits for quantifying adenosine-containing molecules within an aqueous composition. Particularly, the methods utilize a competitive fluorescence-polarization and FRET-based assays that directly measure the production of adenosine-containing compounds, particularly S-adenosylhomocysteine (AdoHcy) compounds produced by MTases. The generation of AdoHcy can be quantified by displacing a novel fluorescent probe comprising a 5-carboxytetramethylrhodamine fluorophore covalently bound to an adenosine scaffold from a catalytically inert 5′-methylthioadenosine nucleosidase (MTAN) variant. One or more of the reaction materials can be pre-loaded into wells within multi-well reaction plates as a kit, which can be used to determine the enzymatic activity of MTases or conduct drug screening for potential inhibitors in a high-throughput format. Additionally, the developed assay is applicable to S-adenosyl methionine-dependent and adenosine triphosphate-dependent enzymes by detecting adenosine and various adenosine-containing molecules including 5′-methylthioadenosine, adenosine monophosphate and adenosine diphosphate produced during the course of a chemical reaction.

公开(公告)号：US20240200040A1

公开(公告)日：2024-06-20

申请号：US17787955

申请日：2019-12-23

申请人： BGI SHENZHEN

发明人： Qun AN ,  Miaomiao GUO ,  Feng XI ,  Fei GUO ,  Yue ZHENG ,  Yuliang DONG ,  Wenwei ZHANG

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/48

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/485 ,  C12Y207/07007

摘要： A chimeric DNA polymerase includes: a first fragment having at least 80% homology to at least part of an N-end domain of KOD DNA polymerase; a second fragment having at least 80% homology to at least part of an exonucleolytic domain of Pab DNA polymerase; a third fragment having at least 80% homology to at least part of the N-end domain of KOD DNA polymerase; a fourth fragment having at least 80% homology to at least part of a palm domain of Pfu DNA polymerase; a fifth fragment having at least 80% homology to at least part of a finger domain of Pab DNA polymerase; a sixth fragment having at least 80% homology to at least part of the palm domain of Pfu DNA polymerase; and a seventh fragment having at least 80% homology to at least part of a thumb domain of KOD DNA polymerase.

公开(公告)号：US20240199948A1

公开(公告)日：2024-06-20

申请号：US18284849

申请日：2022-03-30

申请人： Sumitomo Chemical Co., Ltd.

发明人： Philip Stackhouse ,  Kiran Kamtekar ,  Nazrul Islam

IPC分类号： C09K11/06 ,  C08G61/12 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6869

CPC分类号： C09K11/06 ,  C08G61/122 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6869 ,  C08G2261/148 ,  C08G2261/334 ,  C08G2261/52 ,  C08G2261/94 ,  C09K2211/1483 ,  G01N2333/9126

摘要： A conjugated polymer comprising a first repeat unit substituted with at least three ionic groups.

公开(公告)号：US20240180965A1

公开(公告)日：2024-06-06

申请号：US18348175

申请日：2023-07-06

申请人： SIB Swiss Institute of Bioinformatics ,  CHUV-Centre Hospitalier Universitaire Vaudois ,  Universite de Lausanne ,  Ludwig Institute for Cancer Research Ltd

发明人： Olivier Michielin ,  Vincent Zoete ,  George Coukos ,  Melita Irving ,  Nahzli Dilek ,  Patrick Reichenbach ,  Kelly Ascencao

IPC分类号： A61K35/17 ,  A61K31/517 ,  C07K14/55 ,  C12N9/12 ,  C12N15/10 ,  C12Q1/48 ,  G01N33/573

CPC分类号： A61K35/17 ,  A61K31/517 ,  C07K14/55 ,  C12N9/1205 ,  C12N15/1024 ,  C12Q1/485 ,  G01N33/573 ,  A61K38/00

摘要： The present invention relates to methods of designing kinase mutants for reprogramming the sensitivity of a target kinase to some specific inhibitors, methods of reprogramming the sensitivity of a target kinase to some specific inhibitors, wherein those kinase inhibitors have little or no affinity for the wild-type target kinase, vectors or cells expressing said mutated kinases, composition and uses thereof for the prevention and/or treatment of a disease or disorder, in particular cancer.