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    RARE NUCLEIC ACID DETECTION
    3.
    发明公开
    RARE NUCLEIC ACID DETECTION 审中-公开

    公开(公告)号：US20240309438A1

    公开(公告)日：2024-09-19

    申请号：US18514333

    申请日：2023-11-20

    申请人： Harbinger Health, Inc.

    发明人： Anthony P. Shuber ,  Jennifer L. Steele

    IPC分类号： C12Q1/6848 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6827 ,  C12Q1/6886

    CPC分类号： C12Q1/6848 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12N2310/20 ,  C12N2800/80 ,  C12Q2600/106

    摘要： Methods for detecting rare mutations in DNA include obtaining a sample comprising a target nucleic acid, binding a protein to the target nucleic acid in a sequence-specific manner, digesting non-target nucleic acid in the sample, and detecting the target nucleic acid. The method may include amplifying the target nucleic acid with at least one primer with, e.g., a phosphorothioate bond that is resistant to degradation by a nuclease to yield an amplicon that includes a copy of the target nucleic acid and a terminal portion that is resistant to degradation by the nuclease. Preferably digesting the non-target nucleic acid includes exposing amplicons to the nuclease. The nuclease digests the non-target nucleic acid while the amplicon that includes the copy of the target nucleic acid is protected by the terminal portions and the bound protein.

    A METHOD TO AMPLIFY AND DETECT TARGET NUCLEIC ACIDS WITH VERY HIGH SPECIFICITY AND SENSITIVITY
    4.
    发明公开
    A METHOD TO AMPLIFY AND DETECT TARGET NUCLEIC ACIDS WITH VERY HIGH SPECIFICITY AND SENSITIVITY 审中-公开

    公开(公告)号：US20240279726A1

    公开(公告)日：2024-08-22

    申请号：US18571481

    申请日：2022-06-18

    申请人： OBRONT BIOTECH PRIVATE LIMITED

    发明人： Amirul ISLAM

    IPC分类号： C12Q1/6848 ,  C12Q1/6818

    CPC分类号： C12Q1/6848 ,  C12Q1/6818

    摘要： A method of nucleic acid target detection by nucleic acid amplification is provided including: providing at least one target nucleic acid, at least one oligonucleotide non-extendable or extendable, labelled with at least one shining group/moiety adapted to shine extra upon hybridization or getting incorporated into a nucleic acid molecule; providing at least one extendable oligonucleotide labelled with at least one converter or acceptor group/moiety adapted to convert the color of the shining group/moiety to a different colour or thermalise it, is incorporated into an amplification product; hybridizing or incorporating into a target nucleic acid amplification product the shining group/moiety of the shining group labelled oligonucleotide whereby the shining group/moiety shines extra with the extra shine being a measure of the target amplification. In the method any non-specific amplification does not generate any detectable signal under controlled attenuation by selectively controlling the removing only of the extra shine of the shining group.

    DEVICE AND METHOD FOR PROCESSING BIOLOGICAL SAMPLES
    5.
    发明公开
    DEVICE AND METHOD FOR PROCESSING BIOLOGICAL SAMPLES 审中-公开

    公开(公告)号：US20240271198A1

    公开(公告)日：2024-08-15

    申请号：US18641448

    申请日：2024-04-22

    申请人： STAR ARRAY PTE. LTD.

    发明人： Haiqing GONG

    IPC分类号： C12Q1/6848 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  G01N35/10

    CPC分类号： C12Q1/6848 ,  B01L3/502761 ,  B01L7/52 ,  G01N35/0098 ,  G01N35/1009 ,  B01L2200/04 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/18

    摘要： Cartridge module, apparatus, and method are provided for improving contamination control during biological analyses or nucleic acid analyses protocol. The cartridge module includes a hollow tube and receptacles. A porous cover layer isolates the receptacles from the ambience while the receptacles allow the tube when attached to a syringe to dispense and aspirate a reagent liquid or a biological sample to and from the receptacle through the cover layer only from a receptacle top opening. The cover layer substantially blocks aerosol exchange between the ambience and the receptacle during the whole process of analyses. Ambience contamination due to accidental spillage from the receptacles is also prevented. The porous cover layer reduces change of air pressure inside the receptacles during aspiration or dispensation of liquid.

    SELECTIVE ENRICHMENT
    7.
    发明公开
    SELECTIVE ENRICHMENT 审中-公开

    公开(公告)号：US20240167076A1

    公开(公告)日：2024-05-23

    申请号：US18212371

    申请日：2023-06-21

    申请人： Harbinger Health, Inc.

    发明人： Anthony P. Shuber

    IPC分类号： C12Q1/6806 ,  C12Q1/6848

    CPC分类号： C12Q1/6806 ,  C12Q1/6848

    摘要： Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using selective enrichment. At least two Cas endonuclease complexes are introduced to a sample comprising nucleic acid. The Cas endonuclease complexes comprise guide RNAs and Cas endonuclease. The Cas endonuclease complexes attach to a target nucleic acid, thereby protecting the target of interest while unprotected nucleic acid in the sample is degraded, e.g., by exonuclease digestion. Linkers, when added to the sample, will attach to the ends of the target nucleic acid previously protected by the Cas endonuclease and will not attach to the degraded, unprotected nucleic acid in the sample. The target nucleic acid and linkers are then detected.

    MULTI-FUNCTION ANALYTIC DEVICES
    8.
    发明公开
    MULTI-FUNCTION ANALYTIC DEVICES 审中-公开

    公开(公告)号：US20240132945A1

    公开(公告)日：2024-04-25

    申请号：US18455798

    申请日：2023-08-24

    申请人： Biomeme, Inc.

    发明人： Peter Eisenhower ,  Tom Cook ,  Max Perelman

    IPC分类号： C12Q1/6848 ,  G01N35/00

    CPC分类号： C12Q1/6848 ,  G01N35/00871 ,  G01N21/0332

    摘要： The present disclosure provides devices, systems, methods for processing and/or analyzing a biological sample. An analytic device for processing and/or analyzing one or more biological samples may be electronically and/or physically configured or programed to activate one or more features/operations of the analytic device. The analytic device can be configured or programed by one or more instructions received from a cooperating electronic device or a remote server. The analytic device may comprise a moving carriage. The analytic device may be portable. The analytic device may receive instructions for performing an assay from a mobile electronic device external to a housing of the analytic device.

    EMULSION CHEMISTRY FOR ENCAPSULATED DROPLETS
    10.
    发明公开
    EMULSION CHEMISTRY FOR ENCAPSULATED DROPLETS 审中-公开

    公开(公告)号：US20240093280A1

    公开(公告)日：2024-03-21

    申请号：US18519957

    申请日：2023-11-27

    申请人： Bio-Rad Laboratories, Inc.

    发明人： Amy L. HIDDESSEN ,  Benjamin J. HINDSON

    IPC分类号： C12Q1/6848 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/686

    CPC分类号： C12Q1/6848 ,  C12Q1/6816 ,  C12Q1/6846 ,  C12Q1/686

    摘要： System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.