公开(公告)号：US20240384439A1

公开(公告)日：2024-11-21

申请号：US18592484

申请日：2024-02-29

Applicant: RULES-BASED MEDICINE INC.

Inventor： Andrew Raymon Morton BRADBURY ,  Michael Frank ERASMUS ,  Andre TEIXEIRA

IPC: C40B40/10 ,  C12N15/10 ,  C12N15/81 ,  C40B40/08 ,  C40B50/06

Abstract: Antibody libraries comprising a plurality of heavy chain variable domains and/or a plurality of light chain variable domains, which comprise complementary determining regions (CDRs) found in naturally-occurring human antibodies, and methods of making such antibody libraries. The antibody libraries are free of members that comprise one or more liabilities affecting one or more features of such members. Further, the antibody libraries comprise members having heavy chain and/or light chain CDRs not found in the same naturally-occurring human antibody.

公开(公告)号：US12139752B2

公开(公告)日：2024-11-12

申请号：US16798837

申请日：2020-02-24

Applicant: GENEMO INC.

Inventor： Sheng Zhong ,  Zixu Zhou

IPC: C12Q1/6844 ,  C40B40/08 ,  C40B50/06

Abstract: This invention provides methods for detecting RNAs in a biological sample while using only a small amount of sample input, e.g., less than or equal to 1 mL. The methods described herein are able to detect a large percentage of protein-coding genes having the ENSEMBL gene annotation HG38.

公开(公告)号：US12091776B2

公开(公告)日：2024-09-17

申请号：US16913572

申请日：2020-06-26

Applicant: THE JOHNS HOPKINS UNIVERSITY

Inventor： Harry B. Larman ,  Gabriel Roman-Melendez ,  Thiagarajan Venkataraman

IPC: C40B40/08 ,  C07K17/06 ,  C12N7/00 ,  C12N15/63 ,  C12Q1/6869

CPC classification number: C40B40/08 ,  C07K17/06 ,  C12N7/00 ,  C12N15/63 ,  C12Q1/6869 ,  C07K2317/34 ,  C07K2319/43

Abstract: The present invention relates to the field of proteases. More specifically, the present invention provides compositions and methods useful for profiling protease activity using phage display. In one embodiment, a display vector useful for profiling protease activity comprises a nucleic acid sequence encoding (a) a peptide to be displayed on the surface of the vector; (b) a first affinity tag C-terminal to the peptide; and (c) a second affinity tag N-terminal to the peptide. The display vector can comprise a virus, bacteriophage, yeast, bacteria, retrovirus, ribosome or mRNA. In particular embodiments, the peptide comprises a human peptidome library peptide.

公开(公告)号：US20240240360A1

公开(公告)日：2024-07-18

申请号：US18592456

申请日：2024-02-29

Applicant: RULES-BASED MEDICINE INC.

Inventor： Andrew Raymon Morton BRADBURY ,  Michael Frank ERASMUS ,  Andre TEIXEIRA

IPC: C40B40/10 ,  C12N15/10 ,  C12N15/81 ,  C40B40/08 ,  C40B50/06

CPC classification number: C40B40/10 ,  C12N15/1037 ,  C12N15/81 ,  C40B40/08 ,  C40B50/06

Abstract: Antibody libraries comprising a plurality of heavy chain variable domains and/or a plurality of light chain variable domains, which comprise complementary determining regions (CDRs) found in naturally-occurring human antibodies, and methods of making such antibody libraries. The antibody libraries are free of members that comprise one or more liabilities affecting one or more features of such members. Further, the antibody libraries comprise members having heavy chain and/or light chain CDRs not found in the same naturally-occurring human antibody.

公开(公告)号：US20240240359A1

公开(公告)日：2024-07-18

申请号：US18268538

申请日：2021-12-22

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Alison R. H. Narayan

IPC: C40B40/08 ,  C12P1/00

CPC classification number: C40B40/08 ,  C12P1/00

Abstract: Disclosed herein are methods for synthesizing or functionalizing organic compounds using a library of biocatalysts. The methods include separately admixing a reactant and an aqueous solvent with each biocatalyst in a library of biocatalysts to provide a library of product admixtures, wherein the admixing occurs under sustainable reaction conditions.

公开(公告)号：US20240200131A1

公开(公告)日：2024-06-20

申请号：US18415344

申请日：2024-01-17

Applicant: Fred Hutchinson Cancer Center

Inventor： Jason H. BIELAS

IPC: C12Q1/6874 ,  C12N15/10 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12Q1/6827 ,  C12Q1/6869 ,  C40B40/08 ,  C40B50/06

CPC classification number: C12Q1/6874 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12Q1/6827 ,  C12Q1/6869 ,  C40B40/08 ,  C40B50/06

Abstract: The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

公开(公告)号：US11987904B2

公开(公告)日：2024-05-21

申请号：US16081635

申请日：2017-03-02

Applicant: Aptamer Group Limited

Inventor： Shuji Ikeda ,  Shozo Fujita ,  Tsuyoshi Fujihara

IPC: C40B40/06 ,  C12N15/10 ,  C12N15/115 ,  C40B40/08

CPC classification number: C40B40/08 ,  C12N15/1093 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/52 ,  C12N2320/11 ,  C12N2330/31

Abstract: Oligonucleotide constructs are described, each comprising a functional element and a coding element, wherein the functional element comprises a functional sequence, the functional sequence comprising a sequence of nucleotides in which one or more, or each, nucleotide is modified and the coding element comprises a coding sequence, the coding sequence comprising a sequence of nucleotides which do not contain the modifications of the functional sequence, wherein the coding sequence encodes the sequence structure of the functional sequence.

公开(公告)号：US11970694B2

公开(公告)日：2024-04-30

申请号：US16807435

申请日：2020-03-03

Applicant: PeptiDream Inc.

Inventor： Kenji Kashiwagi ,  Patrick Reid

IPC: C12N15/10 ,  C40B40/08 ,  C40B50/06

CPC classification number: C12N15/1062 ,  C40B40/08 ,  C40B50/06

Abstract: Provided are linkers suitable for preparing a conjugate of a nucleic acid and a peptide as a translation product thereof in a reconstituted cell-free translation system in genotype-phenotype mapping (display methods), said linkers comprising a single-stranded structure region having a side chain base pairing with the base at the 3′-end of an mRNA at one end and a peptidyl acceptor region containing an amino acid attached to an oligo RNA consisting of a nucleotide sequence of ACCA via an ester bond at the other end, characterized in that the ester bond is formed by using an artificial RNA catalyst. Also provided are display methods using [mRNA]-[linker]-[peptide] conjugates assembled via such linkers.

公开(公告)号：US11939355B2

公开(公告)日：2024-03-26

申请号：US17209336

申请日：2021-03-23

Applicant: Howard Hughes Medical Institute ,  The Regents of the University of California

Inventor： Joshua Dudman ,  Adam Hantman ,  Bum-Yeol Hwang ,  Alla Karpova ,  Loren Looger ,  Kimberly Ritola ,  David Schaffer ,  Dougal Gowanlock Robinson Tervo ,  Sarada Viswanathan

IPC: C12N7/00 ,  C07K14/005 ,  C07K14/015 ,  C12N15/86 ,  C40B30/06 ,  C40B40/08 ,  A61K48/00

CPC classification number: C07K14/015 ,  C07K14/005 ,  C12N15/86 ,  C40B30/06 ,  C40B40/08 ,  A61K48/00 ,  C12N2750/14122 ,  C12N2750/14145 ,  C12N2810/6027

Abstract: The present disclosure provides AAV variants that exhibit a preference for retrograde movement in neurons and methods of using such variants.

公开(公告)号：US11802281B2

公开(公告)日：2023-10-31

申请号：US16090829

申请日：2017-03-17

Applicant: ETH ZURICH

Inventor： Sai Reddy ,  William Kelton ,  Cristina Parola ,  Derek Mason ,  Mark Pogson

IPC: C40B20/04 ,  C12N15/10 ,  C12N5/0781 ,  C12N9/22 ,  C12N15/113 ,  C40B10/00 ,  C40B40/08

CPC classification number: C12N15/1037 ,  C12N5/0635 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1058 ,  C12N15/1082 ,  C12N15/1086 ,  C12N15/113 ,  C40B10/00 ,  C40B20/04 ,  C40B40/08 ,  C12N15/1034 ,  C12N2310/20 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2800/80 ,  C12Q2521/539 ,  C12N15/102 ,  C12Q2521/539

Abstract: According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.