公开(公告)号：US12129501B2

公开(公告)日：2024-10-29

申请号：US17903120

申请日：2022-09-06

Applicant: Nuclera Ltd.

Inventor： Michael Chen ,  Jiahao Huang ,  Radu Lazar ,  Gordon McInroy

IPC: C12P19/34 ,  C07H1/02 ,  C07H19/10 ,  C07H19/20 ,  C40B50/04 ,  C40B50/18 ,  C40B80/00

CPC classification number: C12P19/34 ,  C07H1/02 ,  C07H19/10 ,  C07H19/20 ,  C40B50/04 ,  C40B50/18 ,  C40B80/00

Abstract: The invention relates to methods of synthesizing polymers, biopolymers, and nucleic acids, to immobilised dNTP/NTPs and kits comprising said immobilised dNTP/NTPs for use in said methods of nucleic acid synthesis.

公开(公告)号：US20240279845A1

公开(公告)日：2024-08-22

申请号：US18561248

申请日：2022-04-27

Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

Inventor： Christian HEINIS ,  Sevan HABESHIAN ,  Ganesh Kumar MOTHUKURI ,  Mischa SCHUTTEL ,  Manuel MERZ ,  Gontran SANGOUARD ,  Zsolt BOGNAR ,  Alexander Lund NIELSEN

IPC: C40B50/18 ,  C07K1/04

CPC classification number: C40B50/18 ,  C07K1/042 ,  C07K1/047

Abstract: The present invention relates to a method for preparing a library of peptides or an isolated peptide comprising (a) releasing one or more linear dithiol peptides carrying a sulfhydryl group in the N-terminal region of the one or more peptides and being immobilized via a disulfide bridge in the C-terminal region of the one or more peptides on a solid phase from the solid phase by (i) an agent reducing the disulfide bridge, thereby releasing the one or more linear dithiol peptides from the solid phase, wherein the agent is volatile and is removable by evaporation, or (ii) a base that deprotonates the sulfhydryl group in the N-terminal region of the one or more linear dithiol peptides, thereby inducing an intramolecular disulfide exchange thereby releasing the one or more linear dithiol peptides from the solid phase in the form of one or more cyclic peptides.

公开(公告)号：US20240139700A1

公开(公告)日：2024-05-02

申请号：US17816847

申请日：2022-08-02

Applicant: Centrillion Technology Holdings Corporation

Inventor： Maurizio RIGHINI ,  Wei Zhou

IPC: B01J19/00 ,  C40B40/06 ,  C40B50/18

CPC classification number: B01J19/0046 ,  C40B40/06 ,  C40B50/18 ,  B01J2219/00596 ,  B01J2219/00722

Abstract: The present disclosure relates to processes for suspending stretched nucleic acids over surface features. These processes can be used to prepare stretched nucleic acids that are more active in enzymatic reactions and other reactions than those laid down on a flat surface. These processes can be achieved by using a photoresist layer on top of a substrate, stretch a nucleic acid on top of the surface, and then remove part of the photoresist to form surface features that suspend the stretched nucleic acid. Furthermore, the formation of a hydrogel layer over the stretched nucleic acid and the surface features can transfer the stretched nucleic acid to the hydrogel for further reactions, including enzymatic reactions.

公开(公告)号：US20230295696A1

公开(公告)日：2023-09-21

申请号：US18006375

申请日：2020-07-29

Applicant: MGI Tech Co., Ltd.

Inventor： Jay Shafto ,  Radoje Drmanac ,  Snezana Drmanac ,  Chongjun Xu ,  Meihua Gong ,  Ping Wang ,  Xiaojuan Long ,  Wei Zhao ,  Huan Luo ,  Hui Jiang ,  Jian Liu

IPC: C12Q1/6834 ,  C12Q1/686 ,  C40B50/18

CPC classification number: C12Q1/6834 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2600/16

Abstract: Provided are a method for loading a nucleic acid molecule, for example nucleic acid nanoball (e.g., DNA nanoball (DNB)), on a solid support, and a kit for the method.

公开(公告)号：US20230212788A1

公开(公告)日：2023-07-06

申请号：US17913709

申请日：2021-03-24

Applicant: TECHNISCHE UNIVERSITAT DRESDEN

Inventor： Yixin ZHANG ,  Weilin LIN

IPC: C40B50/18 ,  B01J19/00 ,  C07K1/04 ,  G01N33/543

CPC classification number: C40B50/18 ,  B01J19/0046 ,  C07K1/047 ,  G01N33/54393 ,  B01J2219/0061 ,  B01J2219/00603 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00725

Abstract: The invention provides an amphiphilic coating for the direct and rapid synthesis of an array of peptides and small molecular compounds on a planar surface of a solid support, comprising a hydrophilic chemical structure and a lipophilic group, wherein said peptides and small molecular compounds differ from spot to spot from each other in the chemical structure, characterized in that said amphiphilic coating possesses low wettability to polar aprotic solvents used in the array synthesis; said amphiphilic coating possessing low wettability is designed that it can be converted to a coating possessing high wettability by hydrolysis of the lipophilic group; and said amphiphilic coating comprises an amino group for the reaction with an electrophilic reagent. The invention further provides a solid support comprising said amphiphilic coating and a method for method for the direct and rapid synthesis of an array of peptides and small molecular compounds on a planar surface of a solid support, wherein said planar surface of a solid support comprises said amphiphilic coating. Said method includes the enhancing of the wettability of a glass surface to organic solvents to realize automated on-demand biomolecular array synthesis comprising both, peptides and small molecular compounds. The amphiphilic surface can be switched to a hydrophilic surface, resulting in high density arrays suitable for protein- and cell-based screening.

公开(公告)号：US11559778B2

公开(公告)日：2023-01-24

申请号：US16039256

申请日：2018-07-18

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: B01J19/00 ,  C12N15/10 ,  C40B50/18 ,  C40B50/00 ,  C40B50/14 ,  C12N15/66 ,  C12N15/74 ,  C40B40/06 ,  C12N15/09

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US20220098653A1

公开(公告)日：2022-03-31

申请号：US17548246

申请日：2021-12-10

Applicant: ILLUMINA, INC.

Inventor： M. Shane Bowen ,  Bala Murali Venkatesan ,  Hui Han ,  Sang Ryul Park

IPC: C12Q1/6844 ,  C12Q1/6834 ,  C12N15/09 ,  B01J19/00 ,  B01L3/00 ,  C07K1/04 ,  C12Q1/6813 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B50/18

Abstract: Structured substrate including (a) a plurality of nanoparticles distributed on a solid support, (b) a gel material forming a layer in association with the plurality of nanoparticles, and (c) a library of target nucleic acids in the gel material.

公开(公告)号：US11185837B2

公开(公告)日：2021-11-30

申请号：US16535777

申请日：2019-08-08

Applicant: Twist Bioscience Corporation

Inventor： William Banyai ,  Bill James Peck ,  Andres Fernandez ,  Siyuan Chen ,  Pierre Indermuhle

IPC: B01J19/00 ,  C12N15/10 ,  C40B50/18 ,  C40B50/00 ,  C40B40/06 ,  C40B50/14 ,  C12N15/66 ,  C12N15/74 ,  C12N15/09

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

公开(公告)号：US10953379B2

公开(公告)日：2021-03-23

申请号：US16707527

申请日：2019-12-09

Applicant: Illumina Cambridge Limited

Inventor： Mark Edward Brennan Smith ,  Andrea Sabot ,  Isabelle Marie Julia Rasolonjatovo ,  Jean-Ernest Sohna Sohna ,  Adrian Martin Horgan ,  Harold Philip Swerdlow

IPC: C12Q1/6876 ,  C12Q1/6806 ,  B01J19/00 ,  C40B50/18 ,  C12Q1/6834 ,  C40B40/06 ,  C12Q1/6837 ,  C08F222/38

Abstract: Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

公开(公告)号：US10385335B2

公开(公告)日：2019-08-20

申请号：US15101168

申请日：2014-12-05

Applicant: CENTRILLION TECHNOLOGY HOLDINGS CORPORATION

Inventor： Glenn McGall

IPC: C12Q1/68 ,  C12N15/10 ,  C08F220/58 ,  C40B50/18 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6834 ,  C09D4/00 ,  C08F220/56

Abstract: Provided herein are methods and compositions for coating surfaces with polymers. The methods and compositions are suited for conducting biological reactions.