公开(公告)号：US20250067657A1

公开(公告)日：2025-02-27

申请号：US18939263

申请日：2024-11-06

Applicant: Beckman Coulter, Inc.

Inventor： Yong Qin CHEN

IPC: G01N15/1434 ,  F04B11/00 ,  G01N15/10 ,  G01N15/14 ,  G01N35/10 ,  G02B6/26 ,  G02B6/293 ,  G02B21/04 ,  G02B21/36 ,  G02B27/00

Abstract: The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.

公开(公告)号：US12235202B2

公开(公告)日：2025-02-25

申请号：US17847478

申请日：2022-06-23

Applicant: ThinkCyte K.K.

Inventor： Keiji Nakagawa

IPC: G01N15/14 ,  G01N15/10 ,  G01N15/149

Abstract: A method of evaluating performance of a flow cytometer configured to use a combination of two or more types of calibration particles having different morphologies from each other, includes a first classification step of classifying the calibration particles from each other based on a first optical characteristic by the flow cytometer which is an evaluation target, a second classification step of classifying the calibration particles from each other based on a second optical characteristic which is classifiable at a spatial resolution lower than a spatial resolution at which the first optical characteristic is classified, and the evaluation step of evaluating one or both of particle classification performance and a resolution of the flow cytometer based on a first classification result assessed in the first classification step and a second classification result assessed in the second classification step.

公开(公告)号：US12230023B2

公开(公告)日：2025-02-18

申请号：US18511920

申请日：2023-11-16

Applicant: The University of Tokyo ,  OSAKA UNIVERSITY

Inventor： Sadao Ota ,  Issei Sato ,  Katsuhito Fujiu ,  Satoko Yamaguchi ,  Kayo Waki ,  Yoko Itahashi ,  Ryoichi Horisaki

IPC: G01N15/14 ,  C12M1/34 ,  G01N15/1404 ,  G01N15/1429 ,  G01N15/1434 ,  G01N21/01 ,  G01N21/27 ,  G01N21/64 ,  G01N21/65 ,  G06F18/21 ,  G06F18/28 ,  G06V10/772 ,  G06V10/778 ,  G06V10/82 ,  G06V20/69 ,  G01N15/10

Abstract: An analysis device includes an analysis unit configured to receive scattered light, transmitted light, fluorescence, or electromagnetic waves from an observed object located in a light irradiation region light-irradiated from a light source and analyze the observed object on the basis of a signal extracted on the basis of a time axis of an electrical signal output from a light-receiving unit configured to convert the received light or electromagnetic waves into the electrical signal.

公开(公告)号：US20250052742A1

公开(公告)日：2025-02-13

申请号：US18709437

申请日：2021-11-12

Applicant: Beckman Coulter, Inc.

Inventor： Aditya JARARE ,  Rajesh VENKATESH ,  Sumeet CHAWLA ,  Mehul JIVRAJANI ,  Naina ARORA ,  Gopinadh JAKKA ,  Shiva Ranjini SRINIVASAN

IPC: G01N33/53 ,  C09B67/20 ,  C09B69/10 ,  C09K11/02 ,  C09K11/06 ,  G01N15/10 ,  G01N15/14 ,  G01N33/533

Abstract: A novel dry down buffer is provided for use in drying a plurality of fluorescent dye conjugates on a substrate for use in flow cytometry. The aqueous buffer comprises a water-soluble monomer; a protein stabilizer; a carbohydrate stabilizer; and a zwitterionic surfactant. When mixed with a multi-color panel comprising fluorescent polymer dye conjugates, dried on a substrate, and reconstituted with a biological sample, the buffer provides decreased aggregation of fluorescent polymer dye conjugates, and decreased non-specific binding of monocytes and granulocytes, when compared to use of a buffer without the water-soluble monomer or zwitterionic surfactant.

公开(公告)号：US20250033051A1

公开(公告)日：2025-01-30

申请号：US18507075

申请日：2023-11-12

Applicant: CYTOAURORA BIOTECHNOLOGIES, INC.

Inventor： Chung-Er Huang ,  Hsin-Cheng Ho

IPC: B01L3/00 ,  G01N15/10

Abstract: A pico-droplet generator includes a light sensing structure, a mating structure that is spaced apart from the light sensing structure, a bonding layer that connects the light sensing structure and the mating structure, and a piezoelectric member that is disposed on the bonding layer. The pico-droplet generator defines a selection channel along a flowing direction. The bonding layer has a selection hole that is in spatial communication with the selection channel along a dripping direction perpendicular to the flowing direction. The piezoelectric member and the selection hole are respectively arranged on two opposite sides of the bonding layer. The pico-droplet generator has a pico-droplet emission region that is defined from the selection hole toward the piezoelectric member.

公开(公告)号：US12209946B2

公开(公告)日：2025-01-28

申请号：US17094212

申请日：2020-11-10

Applicant: Chi-Kun Oh Yang

Inventor： Tzu-Keng Chiu ,  Yu-Xian Zhu ,  Chi-Kun Oh Yang ,  Cheng-Fang Yang

IPC: G01N15/1434 ,  G01N15/14 ,  G01N15/1429 ,  G01N21/64 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58 ,  G01N15/10

Abstract: A sensing method of bioparticle positioning includes the steps of: providing a carrier divided into multiple detection areas; adding bioparticle sample in the carrier, wherein the bioparticle sample includes first bioparticle with biomarker and interacts with corresponding tag; providing excitation energy that makes the tag on the first bioparticle emit radioactive energy; moving the first sensor to the detection area respectively; after receiving radioactive energy, defining the detection area where the radioactive energy comes from as activity detection area, and sending location information of the activity detection area to processing module; according to location information, moving second sensor to detection area, detecting the accurate location of the first bioparticle in activity detection area, and sending the accurate location to processing module. A bioparticle positioning sensing system is also provided herein. The method and system above detect specific bioparticle quickly and improve the detection efficiency.

公开(公告)号：US12209943B2

公开(公告)日：2025-01-28

申请号：US18567880

申请日：2022-09-16

Applicant: SHANDONG UNIVERSITY

Inventor： Lan Tian ,  Yanqing Wang ,  Jingjing Xu ,  Ming Zeng ,  Wei Liu ,  Xu Tian ,  Xiaoshan Lu

IPC: G01N15/1031 ,  G01N15/10

Abstract: An isolated cell membrane potential detection system can measure a cell membrane current change when light having different parameters such as wavelength, energy density, pulse width, and repetition rate stimulates a cell to be detected, to detect whether the biological electrical activity of the cell can be regulated by light, and to detect the specificity of the light regulation parameters of the cell. In the light regulation cell membrane potential detection system, a method of progressively adjusting and detecting with multiple optical parameters from small to large is used to measure a cell membrane current change in different periods under a certain photostimulation parameter for each group of cells, so as to quickly and efficiently detect which type of optical signal can regulate the characteristics of the cell such as the generation of a membrane current, photostimulation parameter specificity, and an energy threshold.

公开(公告)号：US12196652B2

公开(公告)日：2025-01-14

申请号：US17263800

申请日：2019-08-01

Applicant: Sartorius BioAnalytical Instruments, Inc.

Inventor： Rebecca K. Montange ,  Antje Schickert ,  Jeffrey W. Steaffens ,  Michael W. Olszowy

IPC: G01N1/30 ,  G01N15/14 ,  G01N15/10

Abstract: In flow cytometry evaluation for unassociated virus-size particles stained with multiple fluorogenic dyes, the fluid sample is stained with an aqueous diluted dye formulation including multiple fluorogenic dyes. Preparation of the aqueous diluted dye formulation includes first preparatory processing to prepare a concentrated dye formulation with the fluorogenic dyes provided in a dry powder mixture mixed with and dissolved into a first liquid medium comprising DMSO. After the first preparatory processing, aqueous liquid diluent is added to dilute the first liquid medium and while the fluorogenic dyes remain in solution the liquid medium is converted to an aqueous liquid medium, which may include DMSO as a minor molar component but at a significant concentration. The stained fluid sample may include dissolved disaccharide.

公开(公告)号：US12194461B2

公开(公告)日：2025-01-14

申请号：US17287362

申请日：2019-10-15

Applicant: Technische Universität München

Inventor： Oliver Hayden ,  Rune Barnkob

IPC: B01L3/00 ,  G01N15/00 ,  G01N15/10

Abstract: A method for aggregating a cluster of particles having a target cluster constitution in a channel comprising a retention section comprises the steps of establishing a fluid stream comprising a fluid carrier medium and particles of at least one type through said channel; controlling a supply of said particles of at least one type into the fluid stream; operating a retention mechanism to aggregate at least part of said particles in an aggregation region within said retention section, to thereby form said cluster of particles; monitoring, while operating said retention mechanism, the particles in at least part of said channel for obtaining a monitoring signal associated with the cluster and/or with the particles moving in the fluid stream; determining a current cluster constitution from the monitoring signal; comparing said current cluster constitution with said target cluster constitution; and controlling at least one of said particle retention mechanism and said supply of said particles of at least one type, such that said current cluster constitution approaches said target cluster constitution.

公开(公告)号：US20250012706A1

公开(公告)日：2025-01-09

申请号：US18895402

申请日：2024-09-25

Applicant: FUJIFILM CORPORATION

Inventor： Takashi YAMAMOTO ,  Takayuki NISHI ,  Yuka KOBAYASHI ,  Masataka HASEGAWA

IPC: G01N15/1434 ,  G01N15/01 ,  G01N15/10

Abstract: A flow cell includes: a main body that includes a flow passage through which fluid containing a substance from which physical property data is to be measured flows, and contains a resin; and an optical system which is disposed on a part of a wall surface forming the flow passage and condenses measurement light for the physical property data and of which an emission surface for the measurement light is in contact with the fluid flowing through the flow passage, in which at least a part of an opposite wall surface that is the wall surface facing the optical system and at least a part of a side wall surface that is the wall surface interposed between the opposite wall surface and the optical system are covered with a metal.