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    Aptamer based affinity capture methods for the selective enrichment of human immunoglobulin Fc domains
    2.
    发明授权
    Aptamer based affinity capture methods for the selective enrichment of human immunoglobulin Fc domains 有权

    公开(公告)号：US11572563B2

    公开(公告)日：2023-02-07

    申请号：US16913805

    申请日：2020-06-26

    申请人： Waters Technologies Corporation

    发明人： Xiaoxiao Liu ,  Matthew A. Lauber

    IPC分类号： C12N15/115 ,  C07K16/28 ,  G01N33/561 ,  G01N33/68

    摘要： A method of capturing human immunoglobulin Fc domains in a biofluid sample is provided. The method includes providing an affinity capture device. The affinity capture device includes a surface having an aptamer that is at least 80% identical to SEQ ID NO 1 immobilized onto the surface of the affinity capture device. The biofluid sample is diluted with a binding buffer. The binding buffer includes (A) tris(hydroxymethyl)aminomethane (Tris), trimethylamine (TES), 2-ethanesulfonic acid (MES), or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES); (B) a magnesium cation at a concentration between about 10 μM to about 20 mM; and (C) a total monovalent cation concentration from 0 to no greater than 100 mM. The human immunoglobulin Fc domains in the biofluid sample are adsorbed to the aptamer with the binding buffer.

    Disease detection system and method
    7.
    发明授权
    Disease detection system and method 有权

    公开(公告)号：US10408789B2

    公开(公告)日：2019-09-10

    申请号：US14868328

    申请日：2015-09-28

    申请人： Princeton Biochemicals, Inc.

    发明人： Norberto A. Guzman

    IPC分类号： G01N27/447 ,  G01N33/561 ,  G01N1/40

    摘要： An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.

    IMPROVED IMMUNOFIXATION ELECTROPHORESIS METHOD WITH TARGET COMPONENT ON-GEL IMMUNODISPLACEMENT
    8.
    发明申请
    IMPROVED IMMUNOFIXATION ELECTROPHORESIS METHOD WITH TARGET COMPONENT ON-GEL IMMUNODISPLACEMENT 审中-公开

    公开(公告)号：US20190242891A1

    公开(公告)日：2019-08-08

    申请号：US16320521

    申请日：2017-07-27

    申请人： SEBIA

    发明人： Georges NOUADJE ,  Thierry LIGNEEL

    IPC分类号： G01N33/561 ,  G01N33/68 ,  G01N33/563

    CPC分类号： G01N33/561 ,  G01N33/563 ,  G01N33/6857

    摘要： Disclosed is a method for analyzing biological samples by immunofixation electrophoresis which involves immunodisplacement of target component(s) that may be present in the assayed biological sample, the target component(s) amounting to interfering component(s) when interpretation of the immunofixation results is considered. The immunodisplacement is carried out on an electrophoretic support that is preferably a gel, as defined herein. Accordingly, the invention provides IFE using an antibody or antibodies which is(are) modified (modified antibody) to bear additional negative electric charges, the modified antibody(ies) having antigenic specificity for a predetermined target immunoglobulin.