公开(公告)号：US11639395B2

公开(公告)日：2023-05-02

申请号：US17087313

申请日：2020-11-02

申请人： Genentech, Inc.

发明人： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC分类号： C07K16/28 ,  A61K39/395 ,  C07K1/14 ,  A61P5/00 ,  A61P37/02 ,  C07K14/00 ,  C07K16/32 ,  C12N1/38 ,  C07K1/22 ,  C12N5/00

摘要： The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11584784B2

公开(公告)日：2023-02-21

申请号：US17650570

申请日：2022-02-10

申请人： Cambridge Crops, Inc.

发明人： Amanda Baryshyan ,  Nick Zhang ,  Jesse Groner ,  Adam Behrens ,  Nicole Marco ,  Samantha Roman ,  Rebeca Lopez-Garcia ,  Lindsay Perrea ,  Colin Preston ,  Laith Abu-Taleb ,  Linda Michelle Rauch ,  Herve Irenee Garant, III ,  John Patrick Ellersick

IPC分类号： B01D11/02 ,  B01D61/14 ,  C07K14/435 ,  C07K1/14 ,  B01D63/10 ,  A23L3/3526 ,  B01D61/22 ,  B01D37/04 ,  B01D1/16

摘要： The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The methods include use of a reactor vessel to degum, rinse, and dissolve the silk fibroin protein from silk inputs to obtain a food grade silk powder. The reactor vessel may include a combination of inputs and outputs for introducing and removing different components used or generated during the process. The systems may include any combination of heat exchangers, holding tanks, filtration modules, and post-treatment equipment as necessary to obtain the silk powder. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.

公开(公告)号：US11493412B2

公开(公告)日：2022-11-08

申请号：US16462693

申请日：2017-11-23

申请人： Aman Sharma

发明人： Aman Sharma

IPC分类号： G01N1/40 ,  B01D21/26 ,  C07K1/14 ,  C12N15/10 ,  G01N1/34 ,  G01N1/38 ,  G01N33/49 ,  G01N33/493 ,  G01N33/50

摘要： Methods and kits for isolation of cell-derived vesicles and their associated macromolecules like nucleic acids, proteins, lipids metabolites etc. from one or more blood, serum, plasma, saliva, urine, cerebrospinal fluid, breast milk, tear, conditioned culture media etc. to assist detection, prevention, and understanding of disease biology. The invention offers various advantages including simple technical solutions which are cost-effective, time-saving and scalable for large industrial outputs.

公开(公告)号：US20220340617A1

公开(公告)日：2022-10-27

申请号：US17742243

申请日：2022-05-11

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Christina Passno ,  Christopher Cowan ,  Andrew Tustian

IPC分类号： C07K1/36 ,  C07K1/14 ,  C07K1/34 ,  G01N1/40 ,  G01N21/65

摘要： In situ Raman spectroscopy methods and systems for characterizing or quantifying a protein purification intermediate and/or final concentrated pool during production or manufacture are provided. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify protein purification intermediates critical quality attributes during downstream processing (i.e., after harvest of the protein purification intermediate). For example, the disclosed in situ Raman spectroscopy methods and systems can be used to characterize and quantify protein purification intermediates as the protein purification intermediates are purified, condensed, or otherwise formulated into the final drug product to be sold or administered.

公开(公告)号：US11479593B2

公开(公告)日：2022-10-25

申请号：US17512315

申请日：2021-10-27

申请人： Applied Molecular Transport Inc.

发明人： Derek Maclean ,  Randall J. Mrsny ,  Kevin Yin ,  Tahir Mahmood ,  Bittoo Kanwar ,  Sally Postlethwaite ,  Weijun Feng ,  Sean Dalziel ,  Hyojin Kim ,  Rajendra Tandale ,  Marvin Garovoy ,  John Koleng

IPC分类号： C07K14/54 ,  C07K1/14 ,  C07K1/18 ,  C07K1/34 ,  C07K1/36 ,  C07K14/28 ,  A61K47/32 ,  A61K47/34 ,  A61K47/38 ,  A61K38/00

摘要： Described herein are cholix-IL-10 fusion proteins, and methods of use thereof, which can be characterized by a distinct response in an individual when administered. This distinct response can comprise changes in levels of one or more markers in the individual and/or co-localization of IL-10 in the lamina propria of the individual. Further described herein, in some embodiments, are oral formulations of the cholix-IL-10 fusion proteins. Described herein are methods for the purification of an IL-10 delivery construct, including methods for refolding and enrichment, which can result in maintenance of a high percentage of the IL-10 delivery constructs in the biologically active dimer form. Described herein are oral formulations configured for site-specific release of a therapeutic protein in the small intestines or colon. In some cases, the therapeutic protein is in the form of a dimer, such as an IL-10 delivery construct capable of crossing the gut epithelium.

公开(公告)号：US11439956B2

公开(公告)日：2022-09-13

申请号：US16864234

申请日：2020-05-01

申请人： Fujifilm Diosynth Biotechnologies UK Limited

发明人： Charles Heise ,  Tibor Nagy

IPC分类号： B01D61/18 ,  B01D61/20 ,  C07K1/14 ,  C12N15/10 ,  B01D61/14 ,  B01D61/16 ,  B01D61/22

摘要： Apparatus for in-line liquid exchanging a biomolecule-containing liquid is provided. The apparatus comprises a means for mixing at least two liquids comprising a multiple inlet flow-controller, the means for mixing also comprising an outlet in fluid connection with a tangential flow filtration device configured in single-pass mode.

公开(公告)号：US11427828B2

公开(公告)日：2022-08-30

申请号：US16606486

申请日：2018-04-13

申请人： TOSOH CORPORATION

发明人： Yosuke Terao ,  Naoki Yamanaka ,  Natsuko Kizu ,  Satoshi Endo ,  Seigo Oe

IPC分类号： C07K1/14 ,  C07K1/22 ,  B01D15/38 ,  C07K14/735 ,  C07K16/00 ,  C12N15/09 ,  G01N33/68 ,  C12N15/70 ,  C07K14/435

摘要： The present invention addresses the problem of providing FcRn having improved stability with respect to heat and acids, a method for producing said FcRn, an antibody adsorbent in which said FcRn is used, and an antibody isolation method in which said adsorbent is used. The above problem is solved by substituting an amino acid residue at a specific position in an extracellular region of a human FcRn α chain and/or a β2 microglobulin region of a human FcRn β chain by another specific amino acid.

公开(公告)号：US11420997B2

公开(公告)日：2022-08-23

申请号：US17046682

申请日：2019-04-13

申请人： JITSUBO Co., Ltd.

发明人： Hideaki Suzuki ,  Susumu Muto ,  Shuji Fujita ,  Daisuke Kubo

IPC分类号： C07K1/02 ,  C07K1/06 ,  C07K1/107 ,  C07K1/14 ,  C07K7/06 ,  C07K1/04

摘要： The present invention has an object of shortening the process time and reducing use of a poor solvent for solidifying a carrier (Tag)-peptide component, by removing impurities without conducting solid-liquid separation (condensation, solid-liquid separation and drying operation) of a Tag-peptide component, in an Fmoc method using a Tag for liquid phase peptide synthesis. Provided is the peptide synthesis method that includes the following steps a-d: step a: a carrier-protected amino acid, carrier-protected peptide, or a carrier-protected amino acid amide, and an N-Fmoc-protected amino acid or an N-Fmoc-protected peptide are condensed in an organic solvent or a mixed solution of organic solvents, to obtain an N-Fmoc-carrier-protected peptide, step b: a water-soluble amine is added to the reaction solution after the condensation reaction, step c: the Fmoc group is deprotected from the protected amino group in the presence of a water-soluble amine, and step d: the reaction solution is neutralized by adding an acid, and further, by adding and washing with an acidic aqueous solution, then, by liquid-liquid separation an aqueous layer is removed to obtain an organic layer.

公开(公告)号：US20220213150A1

公开(公告)日：2022-07-07

申请号：US17702985

申请日：2022-03-24

申请人： TAKEDA VACCINES, INC.

发明人： Joel R. HAYNES

IPC分类号： C07K14/005 ,  C12Q1/70 ,  C12N7/00 ,  A61K39/205 ,  C07K1/14

摘要： The present disclosure relates to compositions, methods, mixtures, and kits for detecting the presence of, and for removing, a virus from a product produced in an insect cell. The disclosure also relates to proteins, peptides, polypeptides, drug substances, biological products, vaccine antigens, and virus-like particles that are produced in an insect cell and that are free or substantially free of a virus. The disclosure also relates to compositions, methods, assays, and kits for detecting a rhabdovirus in a sample.

公开(公告)号：US11375741B2

公开(公告)日：2022-07-05

申请号：US15988807

申请日：2018-05-24

申请人： R. J. REYNOLDS TOBACCO COMPANY

发明人： John-Paul Mua ,  Kyle Ford ,  Leigh Ann Blevins Joyce ,  Margarette Elisa Lovette ,  Joshua D. Morton ,  Leigh Hagan ,  Barry Bratcher ,  Samuel Mark Debusk

IPC分类号： A24B15/24 ,  A23L2/66 ,  A61K36/81 ,  A61K8/64 ,  A61Q19/00 ,  A61K8/9789 ,  C07K1/14 ,  C07K14/415

摘要： The present disclosure describes methods of obtaining and/or deriving proteins from plants of the Nicotiana species and methods for incorporation of such proteins into various products. For example, a method for obtaining a protein-enriched material from a plant of the Nicotiana species or portion thereof is provided, comprising: extracting one or more proteins from the plant material into a solvent to form a liquid protein-containing extract; separating a solid extracted plant material from the liquid protein-containing extract; clarifying the liquid protein-containing extract to form a clarified protein-containing extract and a solids fraction; and treating the clarified protein-containing extract so as to provide a protein-enriched material comprising at least about 60% protein by weight.