公开(公告)号：US12122810B2

公开(公告)日：2024-10-22

申请号：US17751277

申请日：2022-05-23

申请人： BOLT THREADS, INC.

发明人： Joshua Kittleson ,  Thomas Stevens ,  Rena Hill ,  Carlos Gustavo Pesce

IPC分类号： C07K14/435 ,  C07K14/395 ,  C12N1/19 ,  C12N15/62 ,  C12N15/81 ,  C12P21/02

CPC分类号： C07K14/43586 ,  C07K14/395 ,  C12N15/625 ,  C12N15/815 ,  C12P21/02 ,  C07K2319/02 ,  C07K2319/036

摘要： The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.

公开(公告)号：US20240344051A1

公开(公告)日：2024-10-17

申请号：US18754121

申请日：2024-06-25

申请人： The Regents of the University of California

发明人： Kathleen Collins ,  Heather E. Upton

IPC分类号： C12N15/10 ,  C07K14/435 ,  C12N9/12

CPC分类号： C12N15/10 ,  C07K14/43563 ,  C12N9/1276 ,  C12N2310/344 ,  C12N2310/345 ,  C12N2310/3517

摘要： The invention provides compositions and methods for nucleic acid synthesis, including ordered and continuous complementary DNA (cDNA) synthesis across non-continuous templates using a modified eukaryotic non-long terminal repeat reverse transcriptase (non-LTR RT) protein.

公开(公告)号：US20240344050A1

公开(公告)日：2024-10-17

申请号：US18754118

申请日：2024-06-25

申请人： The Regents of the University of California

发明人： Kathleen Collins ,  Heather E. Upton

IPC分类号： C12N15/10 ,  C07K14/435 ,  C12N9/12

CPC分类号： C12N15/10 ,  C07K14/43563 ,  C12N9/1276 ,  C12N2310/344 ,  C12N2310/345 ,  C12N2310/3517

摘要： The invention provides compositions and methods for nucleic acid synthesis, including ordered and continuous complementary DNA (cDNA) synthesis across non-continuous templates using a modified eukaryotic non-long terminal repeat reverse transcriptase (non-LTR RT) protein.

公开(公告)号：US12105079B2

公开(公告)日：2024-10-01

申请号：US17269771

申请日：2019-09-11

申请人： Rijksuniversiteit Groningen

发明人： Gang Huang ,  Giovanni Maglia

IPC分类号： G01N33/487 ,  C07K14/435 ,  G01N27/447

CPC分类号： G01N33/48721 ,  C07K14/43595 ,  G01N27/44791 ,  C07K2319/03 ,  C07K2319/21 ,  C07K2319/22

摘要： The invention relates to the field of nanopores, in particular to engineered Fragaceatoxin C (FraC) nanopores and their application in analyzing biopolymers and other (biological) compounds, such as single-molecule (protein) sequencing. Provided is a system comprising oligomeric FraC nanopores comprised in a lipid bilayer, wherein the sum of the nanopore fraction in the heptameric (Type II) state and the nanopore fraction in the hexameric (Type III) state represents at least 60% of the total number of FraC nanopores.

公开(公告)号：US20240317823A1

公开(公告)日：2024-09-26

申请号：US18737141

申请日：2024-06-07

申请人： UNIVERSITY OF WYOMING

发明人： John OAKEY ,  Alan STENQUIST

IPC分类号： C07K14/435 ,  C07K14/75

CPC分类号： C07K14/435 ,  C07K14/75

摘要： The present disclosure generally relates to compositions comprising fibrin and to methods of forming such compositions. In an embodiment, a method of forming fibrin particles is provided. The method includes introducing a buffer, a fibrinogen solution, and a thrombin solution to a first end of a microfluidic device to form a mixture, the buffer comprising one or more amino acids. The method further includes contacting the mixture with a fluorocarbon oil and a surfactant to form fibrinogen-containing particles, and applying positive pressure to the microfluidic device to cause the fibrinogen-containing particles to flow towards a second end of the microfluidic device. The method further includes collecting the fibrinogen-containing particles at the second end of the microfluidic device; and polymerizing the fibrinogen-containing particles to form fibrin particles.

公开(公告)号：US20240316161A1

公开(公告)日：2024-09-26

申请号：US18482248

申请日：2023-10-06

申请人： Bristol-Myers Squibb Company

发明人： Sharon CLOAD ,  Linda ENGLE ,  Dasa LIPOVSEK ,  Malavi MADIREDDI ,  Ginger Chao RAKESTRAW ,  Joanna SWAIN ,  Wenjun ZHAO ,  Hui WEI ,  Aaron P. YAMNIUK ,  Vidhyashankar RAMAMURTHY ,  Alexander T. KOZHICH ,  Martin J. CORBETT ,  Stanley Richard KRYSTEK, JR.

IPC分类号： A61K38/39 ,  A61K38/00 ,  A61K38/17 ,  A61K45/06 ,  A61K47/60 ,  A61K47/64 ,  C07K14/435 ,  C07K14/765 ,  C07K14/78 ,  C07K14/79 ,  C07K16/18 ,  C07K16/46 ,  G01N33/74

CPC分类号： A61K38/39 ,  A61K38/17 ,  A61K38/1709 ,  A61K45/06 ,  A61K47/60 ,  A61K47/642 ,  C07K14/435 ,  C07K14/765 ,  C07K14/78 ,  C07K14/79 ,  C07K16/18 ,  C07K16/46 ,  G01N33/74 ,  A61K38/00 ,  C07K2319/30 ,  C07K2319/31

摘要： The present invention relates to fibronectin-based scaffold domain proteins that bind to myostatin. The invention also relates to the use of these proteins in therapeutic applications to treat muscular dystrophy, cachexia, sarcopenia, osteoarthritis, osteoporosis, diabetes, obesity, COPD, chronic kidney disease, heart failure, myocardial infarction, and fibrosis. The invention further relates to cells comprising such proteins, polynucleotides encoding such proteins or fragments thereof, and to vectors comprising the polynucleotides encoding the proteins.

公开(公告)号：US20240301016A1

公开(公告)日：2024-09-12

申请号：US18264607

申请日：2022-03-23

申请人： FAVORSUN MEDICAL TECH. (SUZHOU) CO., LTD.

发明人： Wenhua YANG ,  Cong OUYANG ,  Yezhuo LIU

IPC分类号： C07K14/435 ,  B01D61/14 ,  B01D61/16

CPC分类号： C07K14/43586 ,  B01D61/145 ,  B01D61/16 ,  B01D2311/04

摘要： A method for preparing a controllable high-concentration silk fibroin solution with high molecular weight using tangential flow ultrafiltration technology is provided. It includes steps of acquiring an inorganic salt solution of silk fibroin; subjecting the inorganic salt solution of silk fibroin to membrane clarification process; desalting the inorganic salt solution of silk fibroin by filtering the clarified solution obtained from the membrane clarification process through a tangential flow filtration system; and concentrating the silk fibroin solution to give a high-concentration aqueous solution of silk fibroin with high molecular weight. The method accomplishes the desalination and concentration of a salt solution of silk fibroin by the tangential flow filtration technology, and directly acquires an aqueous solution of silk fibroin with a number-average molecular weight of 80 kDa-200 kDa.

公开(公告)号：US20240287147A1

公开(公告)日：2024-08-29

申请号：US18037926

申请日：2021-11-29

申请人： Fred Hutchinson Cancer Center ,  Blaze Bioscience, Inc.

发明人： Zachary CROOK ,  James OLSON ,  Natalie Winblade NAIRN ,  Colin CORRENTI

IPC分类号： C07K14/435

CPC分类号： C07K14/43522

摘要： Described herein are cystine-dense peptides capable of binding to PD-L1. Such peptides may function as PD-L1 inhibitors by binding to the PD-1-binding interface of PD-L1 and inhibiting interactions between PD-L1 and PD-1. Methods of using PD-L1-binding peptides to treat cancers, autoimmune diseases, or other conditions are also described herein. Such methods include delivering active agents complexed with a PD-L1-binding peptide to a PD-L1 positive cell or using a bispecific immune cell engager to recruit immune cells to PD-L1 positive cells. Also described herein are chimeric antigen receptors comprising PD-L1-binding peptides.

公开(公告)号：US20240287146A1

公开(公告)日：2024-08-29

申请号：US18499938

申请日：2023-11-01

申请人： AMSILK GmbH ,  Ludwig-Maximilians-Universitat Munchen ,  Universitat Bayreuth

发明人： Lin ROMER ,  Ute SLOTTA ,  Julia ENGERT ,  Gerhard Winter ,  Matthias Lucke ,  Thomas Scheibel

IPC分类号： C07K14/435 ,  A61K38/00 ,  A61K39/00 ,  A61K39/385 ,  A61K47/42 ,  A61K47/64 ,  A61K47/65 ,  A61P35/00 ,  C12N15/62

CPC分类号： C07K14/43518 ,  A61K39/385 ,  A61K47/42 ,  A61K47/646 ,  A61K47/65 ,  A61P35/00 ,  C07K14/43586 ,  C12N15/62 ,  A61K38/00 ,  A61K2039/6031 ,  A61K2039/627 ,  C07K2319/40 ,  C07K2319/50

摘要： The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease.

公开(公告)号：US20240277858A1

公开(公告)日：2024-08-22

申请号：US18174751

申请日：2023-02-27

申请人： Aro Biotherapeutics Company

发明人： Russell C. Addis ,  Zhanna Druzina ,  Robert Kolakowski ,  Swapnil Kulkarni ,  Steven G. Nadler ,  Karyn O'Neil ,  Yao Xin

IPC分类号： A61K47/64 ,  A61K31/7088 ,  C07K14/435 ,  C12N15/113

CPC分类号： A61K47/6435 ,  A61K31/7088 ,  C07K14/435 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/3513 ,  C12N2320/32

摘要： The present disclosure relates to polypeptides, such as fibronectin type III (FN3) domains that can bind CD71, their conjugates, isolated nucleotides encoding the molecules, vectors, host-cells, as well as methods of making and using the same.