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303 条记录 (耗时 0.038 秒)

    Orthogonal process for purification of recombinant human parathyroid hormone (rhPTH) (1-34)
    2.
    发明授权
    Orthogonal process for purification of recombinant human parathyroid hormone (rhPTH) (1-34) 有权
    纯化重组人甲状旁腺激素（rhPTH）（1-34）的正交方法

    公开(公告)号：US08298789B2

    公开(公告)日：2012-10-30

    申请号：US12452919

    申请日：2007-12-06

    申请人： Uma Sankararaman ,  Dipanwita Maiti ,  Meera Sankarankutty ,  Rakesh Shekhawat ,  Narasimha Kumar Kopparapu ,  Gulnaz Zaidi ,  Bipinchandra Rathod ,  Niren Praful Thakar ,  Priti Thakur ,  Anjali Chutke ,  Shrikant Mishra

    发明人： Uma Sankararaman ,  Dipanwita Maiti ,  Meera Sankarankutty ,  Rakesh Shekhawat ,  Narasimha Kumar Kopparapu ,  Gulnaz Zaidi ,  Bipinchandra Rathod ,  Niren Praful Thakar ,  Priti Thakur ,  Anjali Chutke ,  Shrikant Mishra

    IPC分类号： C12N15/16

    CPC分类号： C07K14/635 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/50 ,  C07K2319/61

    摘要： The present invention discloses a process for the preparation of rhPTH (1-34) also known as teriparatide by construction of a novel nucleotide, as an NcoI.IXhoI fragment as set forth in SEQ. ID. No.:1 encoding a chimeric fusion protein as set forth in SEQ.ID. No.:2 comprising of a fusion partner consisting of 41 amino acids belonging to Escherichia coli β-galactosidase (LacZ) gene, an endopeptidase cleavage site, rhPTH (1-34) gene fragment, cloning the said nucleotide in an expression vector under the control of T7 promoter, transforming Escherichia coli with the said vector and expressing the chimeric fusion protein in fed batch fermentation. The present invention further discloses a low feed rate lactose induction for optimized expression of rhPTH (1-34) in Escherichia coli. The present invention also discloses an unique, novel two step orthogonal purification process for rhPTH (1-34) comprising of cation exchange chromatography optionally followed by preparative chromatography selected from HIC or RP-HPLC to yield a target protein of ≧99% purity. The present invention discloses a simple, cost-effective, environmentally benign method of producing high purity rhPTH (1-34).

    摘要翻译： 本发明公开了一种制备rhPTH（1-34）的方法，其也称为特立帕肽，通过构建新的核苷酸，如SEQ ID NO：1所示的NcoI.IXhoI片段。 ID。 编号如SEQ.ID所示的嵌合融合蛋白。 编号：2包含由属于大肠杆菌和半乳糖苷酶（LacZ）基因的41个氨基酸组成的融合配偶体，内肽酶切割位点，rhPTH（1-34）基因片段，将所述核苷酸克隆在表达载体中 T7启动子的控制，用所述载体转化大肠杆菌并在补料分批发酵中表达嵌合融合蛋白。 本发明还公开了用于在大肠杆菌中优化rhPTH（1-34）表达的低进料速率乳糖诱导。 本发明还公开了一种用于rhPTH（1-34）的独特的新型两步正交纯化方法，其包括阳离子交换色谱，任选地随后进行选自HIC或RP-HPLC的制备型色谱，以产生≥99％纯度的靶蛋白。 本发明公开了一种生产高纯度rhPTH（1-34）的简单，成本效益高，环境友好的方法。

    Four-helical bundle protein zsig81
    5.
    发明授权
    Four-helical bundle protein zsig81 失效
    四螺旋束蛋白zsig81

    公开(公告)号：US07223564B2

    公开(公告)日：2007-05-29

    申请号：US10315379

    申请日：2002-12-09

    申请人： Christopher S. Piddington ,  James W. West ,  Richard D. Holly ,  Steven K. Burkhead

    发明人： Christopher S. Piddington ,  James W. West ,  Richard D. Holly ,  Steven K. Burkhead

    IPC分类号： C12N15/16 ,  C12N15/63 ,  C12N5/00 ,  C07K14/475 ,  C07K4/12

    CPC分类号： C07K14/47 ,  C07K14/475

    摘要： This present invention is directed to polypeptide and polynucleotide molecules that encode a four-helical bundle cytokine. The cytokine has been designated zsig81, and has restricted expression in primarily heart, lung and liver. zsig81 has been shown to stimulate proliferation of hematopoietic cells and will be useful expansion of these cells, as well as conditions associated with hematopoietic cells. The invention is directed to antibodies and methods of making zsig81 polypeptides, as well.

    摘要翻译： 本发明涉及编码四螺旋束细胞因子的多肽和多核苷酸分子。 细胞因子被指定为zsig81，并且主要限制在心脏，肺和肝中的表达。 已经显示zsig81刺激造血细胞的增殖，并且这些细胞的有用扩张以及与造血细胞相关的病症。 本发明还涉及制备zsig81多肽的抗体和方法。

    Non-mammalian GnRH analogs and uses thereof in regulation of fertility and pregnancy
    7.
    发明申请
    Non-mammalian GnRH analogs and uses thereof in regulation of fertility and pregnancy 审中-公开
    非哺乳动物GnRH类似物及其在调节生育力和妊娠中的用途

    公开(公告)号：US20050282745A1

    公开(公告)日：2005-12-22

    申请号：US10639748

    申请日：2003-08-12

    申请人： Theresa Siler-Khodr

    发明人： Theresa Siler-Khodr

    IPC分类号： A61K38/00 ,  A61K38/17 ,  C07H21/04 ,  C07K7/23 ,  C07K14/72 ,  C07K16/26 ,  C07K16/28 ,  C12N15/16 ,  C12Q1/68

    CPC分类号： C07K14/72 ,  A61K38/00 ,  C07K16/26

    摘要： Chicken II and salmon GnRH analog decapeptides resistant to degradation by peptidase incorporating D-arginine, D-leucine, D-tBu-Serine, D-Trp or other active D amino acids at position 6 and ethylamide, aza-Gly-amide or other Gly amide at position 10. The analogs demonstrate preferential binding to male and female reproductive system GnRH receptors. Biopotency is greater within the reproductive system than at the pituitary. These non-mammalian GnRH analogs may be used in pharmaceutical preparations, and specifically in various treatment methods as a contraceptive or post-coital contraceptive agent. The non-mammalian GnRH analogs are also provided in pharmaceutical preparations that may be used clinically for maintaining pregnancy when used in very low doses and administered in pulsatile fashion, as well as in preparations for the treatment of male and female reproductive system disorders. The aza-Gly (10) amide non-mammalian analogs are yet other embodiments of the non-mammalian GnRH analogs provided as a part of the invention.

    摘要翻译： 鸡II和鲑鱼GnRH类似物十肽能够通过肽酶降解而掺入D-精氨酸，D-亮氨酸，D-tBu-丝氨酸，D-Trp或其它活性D氨基酸在6位和乙酰胺，氮杂 - 酰胺或其它Gly 酰胺。类似物显示优先结合男性和女性生殖系统GnRH受体。 生殖系统内的生物能力比在垂体方面要高。 这些非哺乳动物GnRH类似物可用于药物制剂，特别是用于避孕或后避孕药的各种治疗方法。 非哺乳动物GnRH类似物也提供在药物制剂中，其可以在临床上用于维持怀孕，当以非常低的剂量使用并以脉动方式施用时，以及用于治疗男性和女性生殖系统疾病的制剂。 作为本发明的一部分提供的非哺乳动物GnRH类似物的另一个实施方案是氮杂-Gly（10）酰胺非哺乳动物类似物。