公开(公告)号：US11903996B2

公开(公告)日：2024-02-20

申请号：US15288830

申请日：2016-10-07

申请人： David C. Fritzinger ,  Daniel E. Benjamin

发明人： David C. Fritzinger ,  Daniel E. Benjamin

IPC分类号： A61K38/17 ,  C07K14/47 ,  A61P37/04 ,  A61K47/10 ,  C12N15/62 ,  C12N15/79 ,  C12N9/64 ,  A61K38/00 ,  C07K14/015

CPC分类号： A61K38/1725 ,  A61K47/10 ,  A61P37/04 ,  C07K14/015 ,  C07K14/47 ,  C07K14/472 ,  C12N15/62 ,  C12N15/79 ,  A61K38/00 ,  C12N9/64 ,  Y02A50/30

摘要： The invention relates generally to a modified human C3 protein containing a number of single amino acid changes in the α and β-chain of human C3 protein, designed to increase the affinity of the modified protein to factor B or Bb, to decrease the affinity of the modified protein to factor H, and to reduce the immunogenicity of the modified protein as compared to the native human C3 protein, a nucleotide sequence encoding the modified C3 protein, a plasmid or viral vector containing the nucleotide sequence for expression the modified C3 protein, and a host cell containing the plasmid or viral vector. We also present a polyethylene glycol covalently bound to the modified C3 protein for reducing immunogenicity and increasing plasma half-life of the modified C3 protein; a method for depleting complement in a patient by administering the modified C3 protein to the patient in an amount effective to deplete complement; a method of ameliorating effects caused by or disease or a method of ameliorating reperfusion injury in a patient by delivering the modified C3 protein.

公开(公告)号：US20240051994A2

公开(公告)日：2024-02-15

申请号：US17802572

申请日：2021-02-25

申请人： Sederma ,  Alganelle

发明人： Ghislaine Tissot-Lecuelle

IPC分类号： C07K7/06 ,  A61K8/64 ,  A61Q19/08 ,  C12N15/79 ,  C07K14/745

CPC分类号： C07K7/06 ,  A61K8/64 ,  A61Q19/08 ,  C12N15/79 ,  C07K14/745 ,  C07K2319/02

摘要： The present invention concerns a recombinant microalgae comprising a nucleic acid sequence encoding a recombinant peptide of KTTKS (SEQ ID N°1); a recombinant peptide, polypeptide or protein consisting in repeated units of SEQ ID N°1; or a derivative thereof, said nucleic acid sequence being located in the chloroplast genome of microalgae. It also relates to a method for producing a recombinant peptide of SEQ ID N°1; a recombinant peptide, polypeptide or protein consisting in repeated units of SEQ ID N°1; or a derivative thereof, wherein said method comprises the chloroplast genome transformation of a microalgae with a nucleic acid sequence encoding said recombinant protein, polypeptide or peptide. It further relates to the use of said recombinant peptide, polypeptide or protein for the cosmetic industry.

公开(公告)号：US11898190B2

公开(公告)日：2024-02-13

申请号：US17401894

申请日：2021-08-13

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ,  KONAN GAKUEN ,  NIPPON SUISAN KAISHA, LTD.

发明人： Yuji Okita ,  Makoto Ito ,  Rie Hamaguchi ,  Hatsumi Goda ,  Seiya Mochinaga ,  Daisuke Honda

IPC分类号： C12P7/6432 ,  C12N15/09 ,  C12P7/64 ,  C12P1/00 ,  C12N15/79 ,  C12P7/6427 ,  C12P7/6472 ,  C12P19/34 ,  C12N1/00 ,  C12N1/12 ,  C12R1/00 ,  C12R1/89 ,  C10G3/00 ,  C12N15/52

CPC分类号： C12P7/6432 ,  C12N15/09 ,  C12N15/79 ,  C12P1/00 ,  C12P7/64 ,  C12P7/6427 ,  C12P7/6472 ,  C12P19/34 ,  C10G3/00 ,  C12N1/00 ,  C12N1/125 ,  C12N15/52 ,  C12R2001/00 ,  C12R2001/89

摘要： A method for producing a microbial oil includes steps of: genetically modifying a labyrinthulid by disrupting and/or silencing a gene, or by transforming another gene in addition to the disruption and/or gene silencing of the gene, and culturing the labyrinthulid, such that a fatty acid composition accumulated in the labyrinthulid comprises an increased EPA content; and collecting the microbial oil having the increased EPA content from the labyrinthulid. The labyrinthulid before the modification is selected from (A) a labyrinthulid belonging to the genus Parietichytrium or genus Schizochytrium and having very weak or no activity of producing PUFAs via a PUFA-PKS pathway; and (B) a labyrinthulid belonging to the genus Thraustochytrium in which a host PUFA-PKS gene is disrupted or silenced to a very weak level. The increased EPA content is preferably not less than 11.5% of a total fatty acid composition.

公开(公告)号：US11840696B2

公开(公告)日：2023-12-12

申请号：US16506201

申请日：2019-07-09

申请人： Novozymes A/S

发明人： Qiming Jin ,  Jeffrey Shasky ,  Donna Moyer ,  Abigail Jang ,  Gloria Muzzi-Erichsen ,  Cara Kleindienst

IPC分类号： C12N15/79 ,  C12N15/90

CPC分类号： C12N15/79 ,  C12N15/902 ,  C12N2800/30

摘要： The present invention relates to methods of introducing multiple expression constructs into a eukaryotic cell, methods of constructing a eukaryotic cell having multiple target loci for expressing multiple heterologous proteins of interest, eukaryotic cells for expressing multiple heterologous proteins of interest, and methods of production of multiple heterologous proteins of interest.

公开(公告)号：US11826433B2

公开(公告)日：2023-11-28

申请号：US16074968

申请日：2017-02-02

申请人： University of Massachusetts

发明人： Miguel Sena Esteves ,  Ana Rita Batista ,  Sourav Roy Choudhury

IPC分类号： A61K48/00 ,  A61K31/7016 ,  A61K31/351 ,  A61K47/64 ,  C12N15/79 ,  C12N15/86 ,  C07K14/775 ,  A61K47/62 ,  A61K31/5415 ,  C12N15/63 ,  A61K38/17 ,  A61K9/00 ,  C12N15/64 ,  A61K31/4706 ,  A61K47/42 ,  C07K5/083

CPC分类号： A61K48/0008 ,  A61K9/0085 ,  A61K31/351 ,  A61K31/4706 ,  A61K31/5415 ,  A61K31/7016 ,  A61K38/1709 ,  A61K47/42 ,  A61K47/62 ,  A61K47/6455 ,  C07K14/775 ,  C12N15/63 ,  C12N15/64 ,  C12N15/79 ,  C12N15/86 ,  C07K5/0808 ,  C07K2319/00 ,  C07K2319/33 ,  C12N2750/14143

摘要： The disclosure relates, in some aspects, to compositions and methods for enhanced delivery of a transgene to the central nervous system (CNS) of a subject. In some embodiments, the transgene is delivered by recombinant AAV (rAAV). In some embodiments, the method of enhancing transgene delivery comprises administering a blood brain barrier (BBB)-crossing molecule (e.g., K16ApoE) and an rAAV comprising a transgene to a subject.

公开(公告)号：US11802149B2

公开(公告)日：2023-10-31

申请号：US18050553

申请日：2022-10-28

申请人： AbbVie Inc.

发明人： Fan Liao ,  Meha Chhaya ,  Andrew J. McCluskey ,  Nathan J. Brown

IPC分类号： C07K16/18 ,  A61P25/28 ,  C12N15/79

CPC分类号： C07K16/18 ,  A61P25/28 ,  C12N15/79 ,  C07K2317/52 ,  C07K2317/565

摘要： The present inventive concept is related to antibodies, such as recombinant humanized and monoclonal antibodies, methods of making antibodies, and methods of using antibodies, such as antibodies directed toward and capable of specifically binding to and clearing amyloid-beta (Aβ) plaques in the brain that are suitable for use in the treatment of disorders such as Alzheimer's Disease (AD).

公开(公告)号：US11801301B2

公开(公告)日：2023-10-31

申请号：US15756936

申请日：2016-09-14

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Karl A. Deisseroth ,  Andre Berndt ,  Soo Yeun Lee ,  Charu Ramakrishnan

IPC分类号： C07K14/00 ,  C12N15/79 ,  C07H21/04 ,  A61K41/00 ,  A61N5/06 ,  C07K14/405 ,  A61K38/00 ,  C12N15/86 ,  C12N15/85

CPC分类号： A61K41/00 ,  A61N5/06 ,  A61N5/062 ,  C07K14/00 ,  C07K14/405 ,  A61K38/00 ,  C07K2319/01 ,  C07K2319/04 ,  C12N15/85 ,  C12N15/86 ,  C12N2750/14143

摘要： The present disclosure provides variant light-responsive polypeptides, and nucleic acids comprising nucleotide sequences encoding the light-responsive polypeptides. The present disclosure provides methods, devices, and systems for controlling the activity of a cell expressing a variant light-responsive polypeptide of the present disclosure.

公开(公告)号：US11793887B2

公开(公告)日：2023-10-24

申请号：US16615941

申请日：2018-05-31

申请人： The Trustees of the University of Pennsylvania ,  University of Southern California ,  The Research Institute of the McGill University Health Centre

发明人： Nancy Braverman ,  Catherine Argyriou ,  Joseph Hacia ,  Jean Bennett ,  Junwei Sun ,  Ji Yun Song ,  Devin McDougald

IPC分类号： A61K48/00 ,  C12N15/79 ,  C07H21/04 ,  A61K35/761 ,  C12N9/14 ,  C12N15/86 ,  C12N15/864

CPC分类号： A61K48/005 ,  A61K35/761 ,  C12N9/14 ,  C12N15/86 ,  C07H21/04 ,  C12N15/8645 ,  C12N2750/14143 ,  C12N2800/22

摘要： Methods and compositions are provided for treatment of peroxisomal biogenesis disorders (PBDs). More particularly, recombinant adeno-associated viruses (rAAV) provided in the form of compositions are used to deliver a nucleic acid encoding human PEX1 to host cells. The rAAVs comprise a AAV capsid, and packaged therein a vector genome comprising an AAV 5′ inverted terminal repeat (ITR) sequence; a promoter; a coding sequence encoding a human PEX1; and an AAV 3′ ITR.

公开(公告)号：US20230332195A1

公开(公告)日：2023-10-19

申请号：US17768834

申请日：2020-10-09

申请人： RIVER STONE BIOTECH APS

发明人： Angela De CARVALHO ,  Swee Chuang Lim HALLWYL ,  Esben Halkjaer HANSEN ,  Jens HOUGHTON-LARSEN

IPC分类号： C12P17/18 ,  C12N15/52 ,  C12N9/02 ,  C12N9/88 ,  C12N9/06 ,  C12N15/79

CPC分类号： C12P17/18 ,  C12N15/52 ,  C12N9/0071 ,  C12N9/88 ,  C12N9/0028 ,  C12N15/79 ,  C12Y114/16002 ,  C12Y402/01078 ,  C12Y114/19 ,  C12Y105/01027

摘要： The invention relate to genetically modified hosts cell comprising a pathway having enhanced production of one or more benzylisoquinoline alkaloids wherein the cell expresses heterologous insect genes encoding insect demethylases converting thebaine into northebaine, thebaine into oripavine, thebaine into nororipavine and/or oripavine into nororipavine.

公开(公告)号：US11753651B2

公开(公告)日：2023-09-12

申请号：US16562560

申请日：2019-09-06

申请人： North Carolina State University

发明人： Rodolphe Barrangou ,  Alexandra Briner Crawley

IPC分类号： C12N9/22 ,  C12N15/113 ,  C12N15/90 ,  C12N15/85 ,  C12N9/96 ,  C12N15/10 ,  C12N15/63 ,  C12N15/79 ,  C12N15/82 ,  C12N15/86 ,  C12N15/11

CPC分类号： C12N15/8509 ,  C12N9/22 ,  C12N9/96 ,  C12N15/102 ,  C12N15/63 ,  C12N15/79 ,  C12N15/8213 ,  C12N15/86 ,  C12N15/907 ,  A01K2217/052 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2800/22

摘要： The present invention is directed to methods and compositions comprising novel CRISPR polypeptides and polynucleotides for site-specific cleavage and nicking of nucleic acids, transcriptional control and genome editing.