公开(公告)号：US20250066726A1

公开(公告)日：2025-02-27

申请号：US18723562

申请日：2022-12-23

Applicant: EWHA UNIVERSITY - INDUSTRY COLLABORATION FOUNDATION ,  HIERABIO INC.

Inventor： Seung Jin LEE ,  Ji-young SHIN ,  Jeong Hwa JEON ,  Heejung KIM

IPC: C12N5/071 ,  A61K35/36 ,  A61P17/02 ,  C12M1/00 ,  C12N5/00

Abstract: The present invention relates to a method for culturing skin-derived stem cells and uses of the same, particularly to a method for culturing skin-derived stem cells; skin-derived stem cells prepared by the method; and a pharmaceutical composition for skin regeneration or wound treatment; a quasi-drug composition; and a cosmetic composition, which contain the prepared skin-derived stem cells, a culture of the skin-derived stem cells, or cells differentiated from the skin-derived stem cells as an active ingredient.
According to the culture method of the present invention, stem cells can be effectively isolated and obtained from skin tissue, and the obtained skin-derived stem cells have excellent pluripotency and self-replication ability as well as excellent skin regeneration and wound treatment effects and can be usefully utilized for skin regeneration and wound treatment or improvement.

公开(公告)号：US20250059545A1

公开(公告)日：2025-02-20

申请号：US18935823

申请日：2024-11-04

Applicant: HOSPITAL ESPAÑOL AUXILIO MUTUO DE PUERTO RICO, INC.

Inventor： Eduardo I. CANTO REPETTI

IPC: C12N15/113 ,  A61K35/742 ,  A61P35/00 ,  C07K16/24 ,  C12N5/00 ,  C12N5/071 ,  C12N5/09 ,  C12Q1/6886 ,  G01N33/50 ,  G01N33/68

Abstract: Disclosed herein is a method for selecting a subject for bladder cancer therapy based on determining from the sample from a subject with bladder cancer, the presence, absence, or level of a biomarker that correlates with interleukin 18 binding protein (IL-18 BP) expression by bladder cells from a subject. Also disclosed herein are compositions comprising an effective amount of at least one of interleukin 18 (IL-18) and an inhibitor of IL-18 binding protein (IL-18 BP), wherein the inhibitor causes at least one of reduces binding of IL-18 BP to IL-18 and reduces IL-18 BP levels. The compositions may be used in methods for treating bladder cancer, comprising administering to a subject in need thereof an effective amount of at least one of IL-18, an inhibitor of IL-18 BP, an antagonist of an activator of IL-18 BP, and an agonist of a down-regulator of IL-18 BP.

公开(公告)号：US20250059506A1

公开(公告)日：2025-02-20

申请号：US18719831

申请日：2022-12-15

Applicant: ZEON CORPORATION

Inventor： Anthony WARD ,  Roberto CAMPOS GONZALEZ ,  Alison SKELLEY ,  Yasna BEHMARDI ,  Khushroo GANDHI ,  Mabel SHEHADA

IPC: C12N5/078 ,  C12N5/00

Abstract: Described herein is a method of enriching lymphocytes from a biological sample, the method comprising: (a) removing large cells and small cells from the biological sample by a size based selection method to obtain lymphocytes, wherein the large cells comprise granulocytes or monocytes, or a combination thereof, and the small cells comprise platelets, red blood cells, or a combination thereof; (b) contacting the lymphocytes with an activating agent to obtain activated lymphocytes; and (c) removing inactivated lymphocytes from the activated lymphocytes using a sized based selection method to obtain activated enriched lymphocytes, thereby obtaining enriched activated lymphocytes.

公开(公告)号：US20250059503A1

公开(公告)日：2025-02-20

申请号：US18722012

申请日：2022-06-20

Applicant: TECHNISCHE UNIVERSITÄT ILMENAU

Inventor： Andreas SCHOBER ,  Jörg HAMPL ,  Dana BRAUER ,  Patrick MAI ,  Leon KAYSAN ,  Lan Vi NGUEN ,  Insa SCHRÖDER

IPC: C12N5/00 ,  C12M1/12 ,  C12M3/06

Abstract: The present invention relates a method for creating organoids, each simulating a biological organ having an organ development state. A substrate is provided, which has a plurality of cavities, each cavity being designed, in its size and form, to receive the organ to be simulated having the organ development state to be achieved. The cavities each have the form of a recess. The recesses have a length and a width, the length being greater than the width. Microfilaments are provided, each having a length which is greater than the width of the recesses and less than the length of the recesses. The individual microfilaments are arranged in the cavities. Living culturable cells are also arranged in the cavities. Conditions provided in the cavities for the culture of the cells on the individual microfilaments. The invention relates to a cell culture and a device for producing an arrangement for creating organoids.

公开(公告)号：US20250059501A1

公开(公告)日：2025-02-20

申请号：US18673522

申请日：2024-05-24

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Reginald Smith ,  Sarah Nicoletti ,  Victor Shashilov ,  Hongxia Wang ,  Jikang Wu ,  Abdelqader Zamamiri

IPC: C12N5/00 ,  C12N5/071 ,  C12N15/79

Abstract: Methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. In certain aspects, methods and media improve protein titer, cell growth, and/or viable cell density.

公开(公告)号：US20250058522A1

公开(公告)日：2025-02-20

申请号：US18720546

申请日：2022-12-16

Applicant: ASPECT BIOSYSTEMS LTD.

Inventor： Simon BEYER ,  Sebastian STEINER ,  Florentin WILFART ,  Shaun LAIDLOW ,  Keddie BROWN ,  Zhensong XU ,  Henrik PERSSON ,  Navid HAKIMI ,  Adam LOOKER

IPC: B29C64/336 ,  B01L3/00 ,  B29C64/118 ,  B29C64/209 ,  B29C64/393 ,  B33Y30/00 ,  B33Y40/00 ,  B33Y50/02 ,  C12N5/00 ,  F04B23/06

Abstract: Aspects of the disclosure include a pump assembly comprising a plurality of pumps (e.g. syringe pumps) positioned in a radial array on a mounting bracket. In embodiments, each syringe pump of the plurality of syringe pumps comprises a housing comprising a syringe retainer and a plunger driver. The pump assembly serves to minimize internal volume when used as part of a multi-channel microfluidic bioprinting system.

公开(公告)号：US12227759B2

公开(公告)日：2025-02-18

申请号：US17126942

申请日：2020-12-18

Applicant: Industrial Technology Research Institute

Inventor： Chia-Jung Lu ,  Jing-En Huang ,  Liang-Cheng Su ,  Hsin-Hsin Shen ,  Yuchi Wang ,  Ying-Hsueh Chao ,  Li-Hsin Lin ,  Hsiu-Hua Huang

IPC: C12N5/00 ,  A61K9/00 ,  A61K35/12 ,  A61K47/38 ,  A61K47/42

Abstract: A temperature-sensitive cell culture composition is provided. The temperature-sensitive cell culture composition includes a hydrogel, a cellulose, a gelatin and a collagen. Based on 1 part by weight of the collagen, a content of the hydrogel is between 0.03 parts by weight and 60 parts by weight, a content of the cellulose is between 150 parts by weight and 360 parts by weight, and a content of the gelatin is between 21 parts by weight and 12 parts by weight. In addition, a method for using the temperature-sensitive cell culture composition, a method for forming the temperature-sensitive cell culture composition, and a use of the temperature-sensitive cell culture composition are also provided.

公开(公告)号：US20250051719A1

公开(公告)日：2025-02-13

申请号：US18685660

申请日：2022-08-23

Applicant: Sartorius Stedim FMT S.A.S ,  Universite Claude Bernard Lyon | ,  Institut National des Sciences Appliquees de Lyon ,  Ecole Superieure de Chimie, Physique, Electronique de Lyon ,  Centre National de la Recherche Scientifique

Inventor： Laura Chastagnier ,  Emma Petiot

IPC: C12N5/00 ,  B33Y10/00 ,  B33Y70/00 ,  C12P1/00

Abstract: Methods and systems for performing a bioproduction process are described, the bioproduction process comprising the production of a transformed cell population. The methods include the steps of culturing a cell population at least partially embedded in a hydrogel matrix forming a three-dimensional structure, the hydrogel matrix also comprising a transforming agent.

公开(公告)号：US20250051706A1

公开(公告)日：2025-02-13

申请号：US18719634

申请日：2022-12-21

Applicant: LEIBNIZ-INSTITUT FÜR POLYMERFORSCHUNG DRESDEN E. V

Inventor： Elisha KRIEG ,  Yu-Hsuan PENG ,  Krishna GUPTA ,  Syuan Ku HSIAO

IPC: C12M1/12 ,  C12N5/00

Abstract: A modifiable hydrogel material has main chain polymers which are modified with anchor modules in the form of predetermined functionalized single strands of DNA. The main chain polymers can be crosslinked with one another by intermolecular DNA double strand formation. A DNA sequence of the anchor modules has a predetermined number of specific sequence positions N with different base combinations and/or is blocked by a temperature-dependent DNA blocking strand. In a method for producing a hydrogel with the modifiable hydrogel material, DNA modules in the form of free DNA single strands or DNA modules in the form of predetermined DNA single strand pairs which bind to anchor modules in a complementary manner are employed for crosslinking the main chain polymers, which form an intermolecular DNA double strand at a common binding domain.

公开(公告)号：US12221720B2

公开(公告)日：2025-02-11

申请号：US16763681

申请日：2018-11-13

Applicant: The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

Inventor： Naomi Habib ,  Aviv Regev ,  Eugene Drokhlyansky ,  Anindita Basu ,  Inbal Avraham-Davidi ,  Orit Rozenblatt-Rosen ,  David A. Weitz

IPC: C40B50/06 ,  A61K35/30 ,  C12N5/0793 ,  C12N5/0797 ,  C12Q1/6806 ,  C12Q1/6881 ,  C40B30/06 ,  C12N5/00

Abstract: Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.