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公开(公告)号：US20230132689A1
公开(公告)日：2023-05-04
申请号：US17915415
申请日：2021-03-30
摘要： The purpose of the present invention is to obtain a cell suspension with a low concentration of cryoprotectant. This method of preserving cells used comprises the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, and (b) freezing the enriched fraction to prepare a frozen material. It is also possible to use a method of producing a cell suspension, comprising the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, (b) freezing the enriched fraction to prepare a frozen material, (c) thawing the frozen material to prepare a thawed material, and (d) mixing the thawed material and a solution to produce a cell suspension.
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公开(公告)号：US11634690B2
公开(公告)日：2023-04-25
申请号：US16481760
申请日：2018-01-31
发明人： Jun Nojima , Hidenori Matsuo , Yuriko Furuya , Hisataka Yasuda
IPC分类号： C12N5/074 , C12N5/0775
摘要： An object of the present invention is to provide a material capable of further accelerating growth of pluripotent stem cells, such as pluripotent stem cells, without impairing pluripotency thereof. In other words, the invention is an agent for accelerating growth of pluripotent stem cells, containing a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, and a solvate thereof as an active ingredient; and is a method for culturing pluripotent stem cells, including culturing pluripotent stem cells in a culture medium that contains a β-nicotinamide mononucleotide or a pharmaceutically acceptable salt thereof, and a solvate thereof.
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公开(公告)号：US11634677B2
公开(公告)日：2023-04-25
申请号：US16845791
申请日：2020-04-10
申请人： Terumo BCT, Inc.
IPC分类号： C12M3/00 , C12M1/00 , C12M1/36 , C12M1/34 , C12Q3/00 , C12N5/0775 , C12M1/12 , B05D7/22 , C12M3/04
摘要： Embodiments described herein generally provide for the expansion of cells in a cell expansion system using an active promotion of a coating agent(s) to a cell growth surface in some embodiments. A coating agent may be applied to a surface, such as the cell growth surface of a hollow fiber in a bioreactor, by controlling the movement of a fluid in which a coating agent is suspended, by changing flow rates, by changing flow directions, by rotation of the bioreactor, and/or combinations thereof.
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公开(公告)号：US11633523B2
公开(公告)日：2023-04-25
申请号：US16294931
申请日：2019-03-07
申请人： FUJIFILM CORPORATION
发明人： Koju Ito , Shun Goto , Souichi Kohashi , Kohji Nakazawa , Hiroshi Yabu
IPC分类号： C12N5/00 , A61L27/56 , C12N5/0775 , C12N5/078 , C12N5/071 , C12N5/077 , C12M1/12 , C12M1/42 , C08J9/28 , C12M3/00
摘要： Provided are a method for producing a cell tissue, including a culturing step of culturing cells capable of serving as a feeder inside opening pores and communicating pores of a porous film having a plurality of the opening pores provided on a surface thereof and the communicating pores communicating mutually adjacent opening pores with one another; and a porous film including a plurality of opening pores provided on a surface thereof and communicating pores communicating mutually adjacent opening pores with one another.
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5.发明申请公开(公告)号：US20230117670A1
公开(公告)日：2023-04-20
申请号：US18066266
申请日：2022-12-14
申请人： Zhejiang University
发明人： Xiao CHEN , Hong ZHANG , Hongwei OUYANG , Zi YIN , Weiliang SHEN
IPC分类号： C12N5/00 , C12N5/0775 , C12N5/077
摘要： The invention provides a bioactive substance composition, a serum-free medium comprising the composition and the uses thereof. The bioactive substance composition is used for serum-free medium and/or composition and the preparation thereof; The serum-free medium and/or composition can be used for primary culture and secondary culture of cells and/or tissues. The cells are selected from any one or more of tendon and/or ligament derived cells, chondrocytes, meniscus stem cells, mesenchymal stem cells, skeleton stem cells, and muscle stem cells. The tissue is the musculoskeletal system tissue. The bioactive substance composition and/or serum-free medium and/or the composition can be used to prepare drugs for tissue and/or organ injury treatment; The tissue or organ injury is selected from the tissue or organ injury of the musculoskeletal system.
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公开(公告)号：US20230117252A1
公开(公告)日：2023-04-20
申请号：US18053847
申请日：2022-11-09
发明人： Goshi SHIOTA , Noriko ITABA , Yohei KONO
IPC分类号： C12N5/0775 , A61K35/28
摘要： [Problem to be solved by the invention] To provide a cell sheet for increasing fibrosis inhibitory action, said cell sheet comprising bone marrow mononuclear cells, and a production method thereof. [Solution] A cell sheet comprising bone marrow mononuclear cells, said cell sheet being obtained by forming bone marrow mononuclear cells from a bone marrow mononuclear cell suspension into a sheet, and then shrinking and suspension culturing the result.
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公开(公告)号：US20230116841A1
公开(公告)日：2023-04-13
申请号：US17907318
申请日：2021-04-02
发明人： Jin JIN
IPC分类号： C12N5/0775 , C12Q1/24
摘要： Provided are a method for obtaining endometrial mesenchymal stem cells from human menstrual blood and the endometrial mesenchymal stem cells prepared thereby. The method include: 1) subjecting an aliquot of menstrual blood to cascade filtration through graded cell strainers of decreasing mesh sizes to separate tissue debris, wherein said cell strainers include a filter-through cell strainer for collecting filtrate and a retention cell strainer for collecting retentate, wherein the mesh size of the retention cell strainer is smaller than that of the filter-through cell strainer; 2) collecting the retentate on the retention cell strainer as the tissue debris; 3) culturing the tissue debris in a mesenchymal stem cells medium; and 4) collecting adherent cells as the endometrial mesenchymal stem cells.
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8.发明申请公开(公告)号：US20230115023A1
公开(公告)日：2023-04-13
申请号：US17938607
申请日：2022-10-06
IPC分类号： A61K35/28 , C12N5/0775 , A61P1/16
摘要： The present disclosure provides a method for treating non-alcoholic steatohepatitis and/or non-alcoholic fatty liver disease in a subject in need thereof, the method comprises administering exosome, an exosome pellet, or physiological solution derived from mesenchymal stem cells (MSCs) to the subject.
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公开(公告)号：US20230103075A1
公开(公告)日：2023-03-30
申请号：US17880141
申请日：2022-08-03
发明人： Richard Klemke , Huawei Wang , Willie Pi
IPC分类号： A61K9/50 , C12N5/0775 , A61K45/06 , A61K9/00
摘要： Provided are methods for treating a disease using bioengineered enucleated cells. Also provided herein are compositions comprising enucleated cells, wherein the enucleated cells have been loaded with clinically relevant biomolecules.
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公开(公告)号：US11608490B2
公开(公告)日：2023-03-21
申请号：US16427084
申请日：2019-05-30
发明人： Jong Hyuk Sung , Na Hyun Choi
IPC分类号： C12N5/0775 , A61K35/28 , A61K38/20 , A61P17/14
摘要： The present invention relates to a hair growth-inducing ability enhancing effect of adipose stem cells obtained by performing treatment with udenafil and performing culture, and provides a composition for preventing or treating alopecia, or promoting hair growth, comprising, as an active ingredient, adipose stem cells obtained by performing treatment with udenafil and performing culture, IL-4, or IL-12B. In a case where a culture medium of adipose stem cells is treated with udenafil and cultured, IL-4 and IL-12B expression levels are increased in the adipose stem cells, and thus maturation of hair follicle cells is further promoted, so that hair growth can be induced. In addition, in a case where IL-4 and IL-12B, which are expressed in the adipose stem cells obtained by performing treatment with udenafil and performing culture, are applied to skin tissue, hair growth can be induced.
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