公开(公告)号：US20240360459A1

公开(公告)日：2024-10-31

申请号：US18437991

申请日：2024-02-09

申请人： Nankai University

发明人： Hongri Gong ,  Yuying Zhang ,  Zihe Rao

IPC分类号： C12N15/70 ,  C07K14/35 ,  C12N1/20 ,  C12N9/00 ,  C12R1/19

CPC分类号： C12N15/70 ,  C07K14/35 ,  C12N1/20 ,  C12N9/00 ,  C12R2001/19

摘要： The present disclosure provides a Mycobacterium tuberculosis (Mtb) adenosine triphosphate (ATP) synthase-expressing recombinant bacterium, and a construction method and an expression method thereof, and belongs to the technical field of exogenous expression. The present disclosure provides a construction method of an Mtb ATP synthase-expressing recombinant bacterium, including the following steps: using a Mycobacterium smegmatis (Msm) competent cell containing an auxiliary gene knockout plasmid as a basal cell; transferring an Mtb ATP synthase gene cluster with an affinity purification tag into the basal cell, knocking out a Msm ATP synthase genome, and collecting a strain without the auxiliary gene knockout plasmid after conducting repeated subculture; and transferring the Mtb ATP synthase gene cluster into the strain by prokaryotic expression to obtain the Mtb ATP synthase-expressing recombinant bacterium.

公开(公告)号：US20240360422A1

公开(公告)日：2024-10-31

申请号：US18287734

申请日：2022-04-21

申请人： MICHAEL T. LEONARD ,  Jeremy j. Gam ,  Christopher S. Stach ,  Alec A.K. Nielsen

发明人： MICHAEL T. LEONARD ,  Jeremy j. Gam ,  Christopher S. Stach ,  Alec A.K. Nielsen

IPC分类号： C12N7/00 ,  C07K14/005 ,  C07K14/435 ,  C12N9/00 ,  C12N15/86

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K14/435 ,  C12N9/93 ,  C12N15/86 ,  C12Y601/01026 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152

摘要： Disclosed herein are cell genetically engineered cell for AAV production. The genetically engineered cell comprises molecular systems for temporal control of expression of genes required for AAV production. Also disclosed herein are methods of using genetically engineered cells for AAV production.

公开(公告)号：US12116570B2

公开(公告)日：2024-10-15

申请号：US16337282

申请日：2017-09-28

申请人： Roche Sequencing Solutions, Inc.

发明人： Mark Stamatios Kokoris ,  Michael Kovarik ,  Marc Prindle ,  Salka Keller ,  Robert Busam ,  Miranda Lahman

IPC分类号： C12N15/10 ,  C12N1/20 ,  C12N9/00 ,  C12N9/12 ,  C12N15/74 ,  C40B20/04 ,  C40B40/08

CPC分类号： C12N15/1058 ,  C12N1/20 ,  C12N9/1252 ,  C12N9/93 ,  C12N15/74 ,  C40B20/04 ,  C40B40/08 ,  C12Y207/07007

摘要： Methods and compositions for the selection of nucleic acid processing and other enzymes, and more specifically for the selection of DNA polymerases and other enzymes with desired properties employing the directed evolution of enzymes by plasmid tagging in droplets.

公开(公告)号：US20240318212A1

公开(公告)日：2024-09-26

申请号：US18599745

申请日：2024-03-08

申请人： National Taiwan University

发明人： Nan-Wei SU ,  Hsin-Ya TSAI ,  Chen HSU

IPC分类号： C12P17/18 ,  C12N9/00 ,  C12N9/12 ,  C12P17/06

CPC分类号： C12P17/181 ,  C12N9/1229 ,  C12N9/93 ,  C12P17/06 ,  C12Y207/04001 ,  C12Y602/01005

摘要： The present invention provides a coupled enzyme system, comprises: a first enzyme, comprising a polyphenol phosphorylation synthetase; a second enzyme, which is ATP regeneration enzyme; and a substrate, being phosphorylated by the first enzyme. The coupled enzyme system of the present invention integrates polyphenol phosphorylation synthetase with ATP regeneration enzyme so that the polyphenol phosphorylation synthetase is used to phosphorylate polyphenol and the ATP regeneration enzyme regenerate ATP from AMP. Therefore, the present invention not only improves the water-solubility and bioavailability of the phenolic phytochemicals but also significantly reduces ATP consumption, presenting the potential of enzymatic systems in the production of polyphenol monophosphates.

公开(公告)号：US20240316130A1

公开(公告)日：2024-09-26

申请号：US18609666

申请日：2024-03-19

申请人： UNM Rainforest Innovations

发明人： Eric Bartee

IPC分类号： A61K35/768 ,  C07K14/54 ,  C07K14/705 ,  C12N9/00 ,  C12N9/10 ,  C12N9/88 ,  C12N15/86

CPC分类号： A61K35/768 ,  C07K14/5434 ,  C07K14/70521 ,  C12N9/1018 ,  C12N9/88 ,  C12N9/93 ,  C12N15/86 ,  C12Y201/03003 ,  C12Y403/02001 ,  C12Y603/04005 ,  C12N2710/24032 ,  C12N2710/24043

摘要： A replication-competent recombinant virus includes an expression cassette encoding a protein associated with arginine biosynthesis. The protein associated with arginine biosynthesis may include argininosuccinate synthase 1 (ASS1), argininosuccinate lyase (ASL), or ornithine transcarboxylase (OTC). The recombinant virus may be derived from a myxoma virus. A method of treating a cell includes contacting a cell with a recombinant virus including an expression cassette encoding a protein associated with arginine biosynthesis. The protein associated with arginine biosynthesis may be ASS1, ASL, OTC.

公开(公告)号：US12084690B2

公开(公告)日：2024-09-10

申请号：US18488060

申请日：2023-10-17

申请人： LINNO PHARMACEUTICALS INC.

发明人： Zhaozhong Han ,  Hongya Pan ,  Haiyang Li ,  Lingyu Li

IPC分类号： A61K39/395 ,  A61K38/43 ,  A61P25/16 ,  A61P25/28 ,  C07K16/18 ,  C12N9/00 ,  C12N9/16 ,  C12N15/63

CPC分类号： C12N9/16 ,  A61P25/16 ,  A61P25/28 ,  C07K16/18 ,  C12N15/63 ,  C07K2317/565 ,  C07K2317/94

摘要： The present application pertains to a fusion protein, comprising a transferrin-binding protein, and an arylsulfatase A (ARSA) or functionally active fragment thereof, wherein the transferrin-binding protein comprises a peptide, an antibody or an antigen-binding fragment of the antibody capable of binding transferrin. The other aspect of the present application provides the preparing methods and the uses of the fusion protein.

公开(公告)号：US20240294927A1

公开(公告)日：2024-09-05

申请号：US17768618

申请日：2020-10-27

申请人： CHRYSEA LIMITED

发明人： Jiufu Qin ,  Jens Nielsen

IPC分类号： C12N15/52 ,  C12N1/16 ,  C12N9/00 ,  C12N9/10 ,  C12P21/02

CPC分类号： C12N15/52 ,  C12N1/165 ,  C12N9/1085 ,  C12N9/93 ,  C12P21/02 ,  C12Y205/01022 ,  C12Y205/01044 ,  C12Y205/01079 ,  C12Y603/01008 ,  C12Y603/01009

摘要： The invention relates to production of polyamine conjugates in yeast cells that are capable of producing at least one polyamine. The yeast cells also comprise a polyamine:glutathione ligase encoding gene and at least one polyamine synthase encoding gene but lacks a polyamine oxidase encoding gene or comprises a disrupted polyamine oxidase encoding gene. The yeast cells are capable of producing various polyamine-glutathione conjugates.

公开(公告)号：US12070476B2

公开(公告)日：2024-08-27

申请号：US17584508

申请日：2022-01-26

申请人： Ramot at Tel-Aviv University Ltd. ,  The University Court of the University of Glasgow

发明人： Oded Rechavi ,  Shahar Bracha ,  Lilach Sheiner

IPC分类号： A61K35/68 ,  C07K14/45 ,  C12N9/00 ,  C12N15/65 ,  C12N15/79

CPC分类号： A61K35/68 ,  C07K14/45 ,  C12N9/93 ,  C12N15/65 ,  C12N15/79 ,  C07K2319/10 ,  C12Y603/05005

摘要： Provided are nucleic acid constructs, Toxoplasma comprising same, pharmaceutical compositions comprising same and methods using same for delivering a protein-of-interest to a tissue-of-interest of a subject, such as the CNS and further treating a pathology which is treatable by administration of a therapeutic polypeptide in a central nervous system of the subject.

公开(公告)号：US20240279705A1

公开(公告)日：2024-08-22

申请号：US18681159

申请日：2022-07-29

申请人： Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e. V.

发明人： Jeffrey SCHLOSSHAUER ,  Anne ZEMELLA ,  Stefan KUBICK ,  Nino CAVAK ,  Lena THORING

IPC分类号： C12P21/02 ,  C12N9/00 ,  C12N9/12

CPC分类号： C12P21/02 ,  C12N9/127 ,  C12N9/93 ,  C12Y207/07048 ,  C12Y601/01001 ,  C12Y601/01004 ,  C12Y601/01026

摘要： The present invention relates to a method for producing eukaryotic cell lysates for cell-free protein synthesis comprising at least one exogenous enzyme, wherein the method comprises at least the following steps: a) providing eukaryotic cells transfected with at least one donor template coding for at least one exogenous enzyme which is selected from the group comprising orthogonal aminoacyl-tRNA synthetases and viral RNA polymerases: b) cultivating the transfected cells of step a) for a predetermined period of time and subsequently harvesting the cells; and c) disrupting the harvested cells and preparing a cell lysate for cell-free protein synthesis therefrom. More specifically, the invention relates to a method for producing eukaryotic cell lysates which are capable of cell-free synthesis of a target protein comprising a non-canonical amino acid, wherein the method comprises at least the following steps: a) providing eukaryotic cells transfected with at least one donor template coding for an orthogonal aminoacyl-tRNA synthetase. i.e. an aminoacyl-tRNA synthetase which is specific for a tRNA which is not recognized by endogenous aminoacyl-tRNA synthetase in said eukaryotic cells and is specific for a corresponding non-canonical amino acid: b) cultivating the transfected cells of step a) for a predetermined period of time and subsequently harvesting the cells; and c) disrupting the harvested cells and preparing a cell lysate therefrom. Further aspects of the present invention relate in particular to eukaryotic cell lysates obtainable by the above methods as well as to a method for performing cell-free synthesis of a target protein comprising a non-canonical amino acid.

公开(公告)号：US20240263209A1

公开(公告)日：2024-08-08

申请号：US18606513

申请日：2024-03-15

申请人： Absci Corporation

发明人： Yi Zhang ,  Matthew Weinstock ,  Miles Gander

IPC分类号： C12P21/00 ,  C12N9/00 ,  C12N15/11 ,  C12N15/70 ,  C12R1/19

CPC分类号： C12P21/00 ,  C12N9/93 ,  C12N15/11 ,  C12N15/70 ,  C12R2001/19 ,  C12Y601/01001

摘要： This disclosure provides engineered tRNAs and the corresponding aminoacyl tRNA synthetase for efficient production of proteins containing non-standard amino acids. These engineered orthogonal tRNA (O-tRNA)/orthogonal aminoacyl tRNA synthetase (O-RS) pair, i.e., Orthogonal Translation Systems (OTSs) can be used to incorporate a non-standard amino acid in a specific position in a growing polypeptide in response to a selector codon that is recognized by the engineered tRNA.