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    Acinetobacter and use thereof in production of chiral 3-cyclohexene-1-carboxylic acid
    4.
    发明授权
    Acinetobacter and use thereof in production of chiral 3-cyclohexene-1-carboxylic acid 有权

    公开(公告)号：US11441133B2

    公开(公告)日：2022-09-13

    申请号：US15734969

    申请日：2019-11-22

    申请人： JIANGNAN UNIVERSITY

    发明人： Ye Ni ,  Zhe Dou ,  Guochao Xu

    IPC分类号： C12P7/40 ,  C12N9/18

    摘要： The invention discloses a strain of Acinetobacter and use thereof in the production of chiral 3-cyclohexene-1-carboxylic acid. Its taxonomic name is Acinetobacter sp., which is deposited on Jan. 21, 2019 at the China General Microbiological Culture Collection Center, under accession number CGMCC No. 17220. Using the Acinetobacter strain of the invention to produce chiral methyl 3-cyclohexene-1-carboxylate, the resulting methyl (S)-3-cyclohexene-1-carboxylate has an optical purity of 99% or more, and the catalyst has good stability, mild reaction condition and can withstand high concentrations of substrate and product. Using the resolution process of the invention, (S)-3-cyclohexene-1-carboxylic acid with high optical purity and high concentration can be simply and efficiently obtained, and the process is energy-saving and environmentally friendly, and the high-concentration of product is beneficial to downstream product recovery process. The invention provides an efficient method for production of (S)-3-cyclohexene-1-carboxylic acid, and has a good industrial application prospect.

    Method of producing lipid
    10.
    发明授权
    Method of producing lipid 有权

    公开(公告)号：US11274322B2

    公开(公告)日：2022-03-15

    申请号：US16650054

    申请日：2018-10-03

    申请人： Kao Corporation

    发明人： Akihito Kawahara

    IPC分类号： C12P7/64 ,  C12N9/02 ,  C12N9/18 ,  C12P7/6463

    摘要： A method of producing lipids, containing the steps of:
    culturing a transformant into which a gene encoding at least one protein selected from the group consisting of the following proteins (A) to (C) is introduced; and
    producing fatty acids or lipids containing the same as components: (A) a protein consisting of an amino acid sequence having at least one amino acid substitution selected from the group consisting of the following (A-1) to (A-11) in the amino acid sequence set forth in SEQ ID NO: 1, and having acyl-ACP thioesterase activity; (B) a protein consisting of an amino acid sequence having at least one amino acid substitution selected from the group consisting of the following (B-1) to (B-11) in an amino acid sequence having 85% or more identity with the amino acid sequence set forth in SEQ ID NO: 1, and having acyl-ACP thioesterase activity; and (C) a protein containing the amino acid sequence of the protein (A) or (B), and having acyl-ACP thioesterase activity: (A-1) substitution of isoleucine for leucine at position 257 of the amino acid sequence set forth in SEQ ID NO: 1; (A-2) substitution of arginine for threonine at position 251 of the amino acid sequence set forth in SEQ ID NO: 1; (A-3) substitution of lysine for threonine at position 251 of the amino acid sequence set forth in SEQ ID NO: 1; (A-4) substitution of histidine for threonine at position 251 of the amino acid sequence set forth in SEQ ID NO: 1; (A-5) substitution of isoleucine for tryptophan at position 254 of the amino acid sequence set forth in SEQ ID NO: 1; (A-6) substitution of tyrosine for tryptophan at position 254 of the amino acid sequence set forth in SEQ ID NO: 1; (A-7) substitution of methionine for leucine at position 257 of the amino acid sequence set forth in SEQ ID NO: 1; (A-8) substitution of valine for leucine at position 257 of the amino acid sequence set forth in SEQ ID NO: 1; (A-9) substitution of phenylalanine for leucine at position 257 of the amino acid sequence set forth in SEQ ID NO: 1; (A-10) substitution of cysteine for valine at position 266 of the amino acid sequence set forth in SEQ ID NO: 1; (A-11) substitution of tyrosine for tryptophan at position 271 of the amino acid sequence set forth in SEQ ID NO: 1; (B-1) substitution of isoleucine for an amino acid at a position corresponding to position 257 of the amino acid sequence set forth in SEQ ID NO: 1; (B-2) substitution of arginine for an amino acid at a position corresponding to position 251 of the amino acid sequence set forth in SEQ ID NO: 1; (B-3) substitution of lysine for an amino acid at a position corresponding to position 251 of the amino acid sequence set forth in SEQ ID NO: 1; (B-4) substitution of histidine for an amino acid at a position corresponding to position 251 of the amino acid sequence set forth in SEQ ID NO: 1; (B-5) substitution of isoleucine for an amino acid at a position corresponding to position 254 of the amino acid sequence set forth in SEQ ID NO: 1; (B-6) substitution of tyrosine for an amino acid at a position corresponding to position 254 of the amino acid sequence set forth in SEQ ID NO: 1; (B-7) substitution of methionine for an amino acid at a position corresponding to position 257 of the amino acid sequence set forth in SEQ ID NO: 1; (B-8) substitution of valine for an amino acid at a position corresponding to position 257 of the amino acid sequence set forth in SEQ ID NO: 1; (B-9) substitution of phenylalanine for an amino acid at a position corresponding to position 257 of the amino acid sequence set forth in SEQ ID NO: 1; (B-10) substitution of cysteine for an amino acid at a position corresponding to position 266 of the amino acid sequence set forth in SEQ ID NO: 1; and (B-11) substitution of tyrosine for an amino acid at a position corresponding to position 271 of the amino acid sequence set forth in SEQ ID NO: 1.