BIOCOMPATIBLE UNDERLAYS FOR LIVING EXTRACTION OF HYDROCARBONS FROM ENGINEERED MICROBES

    公开(公告)号:US20240360481A1

    公开(公告)日:2024-10-31

    申请号:US18688207

    申请日:2022-08-30

    摘要: Methods and systems that use fluorocarbons for designing scalable living, in-line extraction of heterologous metabolites from microbial cultures are disclosed. The disclosed systems and methods are organism agnostic and be applied to bacteria, yeasts, filamentous microbes, and algae. The disclosed methods and systems use pperfluorocarbon solvent to extract terpenes from engineered microorganisms and allows non-lethal milking of hydrocarbon products from filamentous and single-celled/multicelled microbial cultures. The disclosed process preferably does not use pressure gradients and relies on the physical interaction of microbes with fluorocarbon at satp and standard temperature of microbial cultivation to partition non-native products. The disclosed process and system also allow for increased production of products providing a physical sink which enables the forward reactions to produce more products than in its absence.

    METHOD OF MAKING GRAPHENE TRANSPARENT BY FERMENTATION

    公开(公告)号:US20240360479A1

    公开(公告)日:2024-10-31

    申请号:US18140097

    申请日:2023-04-27

    申请人: Zhi-Min Li

    发明人: Zhi-Min Li

    IPC分类号: C12P3/00 A61K33/44 A61K35/741

    摘要: A method of making graphene transparent by fermentation contains: a step of preparing prerequisites; a first step in which the graphene mother bacteria, edible powders, powdered sugars, and a warm water and mixed and heated to produce a fermented graphene lump; a second step in which fermented graphene lump is smashed and smoothed out to a holding tray, then the holding tray is dried to form graphene enzyme lump. The method further contains: a third step in which the graphene enzyme lump is refined to graphene enzyme powders by a stirrer; a fourth step in which re-fermenting the graphene enzyme powders of the third step by repeating the first step, the second step and the third step; a fifth step in which the re-fermented graphene powders are soaked to produce edible powders, and the warm water is removed; a step sixth in which a transparent graphene containing probiotics is produced.