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公开(公告)号：US11999976B2
公开(公告)日：2024-06-04
申请号：US17290916
申请日：2019-12-11
发明人： Haibin Chen , Chuanyang Shang , Baoqin Cai , Marco Bocola , Shumin Shi , Ruimei Hong , Lei Sun , Yong Koy Bong
CPC分类号： C12N9/0006 , C12N11/02 , C12P7/22 , C12P7/62 , C12P41/002 , C12Y101/01184
摘要： Provided are amino acid sequences of ketoreductase polypeptides that are useful for asymmetrically synthesizing chiral alcohol compounds, its preparation process as well as reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered ketoreductase polypeptides, engineered host cells capable of expressing engineered ketoreductase polypeptides, and methods of producing chiral alcohol compounds using engineered ketoreductase polypeptides. Compared to other enzymes, the engineered ketoreductase polypeptides provided by the invention have better catalytic activity and thermal stability, allowing purification of the enzyme solution by heat treatment, which is advantageous for the production of enzymes and the industrial application of enzymatic reactions. The use of the engineered polypeptides of the present invention greatly simplifies the production process of chiral alcohol compounds, reduces the cost of production and the impact on the environment, and has good industrial application prospects.
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公开(公告)号：US20240150344A1
公开(公告)日：2024-05-09
申请号：US17769241
申请日：2020-10-12
IPC分类号： C07D471/04 , C07B57/00 , C12N9/20 , C12P41/00
CPC分类号： C07D471/04 , C07B57/00 , C12N9/20 , C12P41/00 , C07B2200/07 , C12Y301/01003
摘要： The present invention relates to a process for preparing acyloxymethyl esters of (4S)-(4-cyano-2-methoxyphenyl)-5-ethoxy-2,8-dimethyl-1,4-dihydro-1,6-naphthyridine-3-carboxylic acid of the formula (IIa) by optical resolution of the compound of the formula (II) using a hydrolase. The invention also relates to a process for preparing (4S)-4-(4-cyano-2-methoxyphenyl)-5-ethoxy-2,8-dimethyl-1,4-dihydro-1,6-naphthyridine-3-carboxamide of the formula (Ia), wherein the process comprises the optical resolution of the compound of the formula (II) using a hydrolase. The invention additionally also relates to the use of a hydrolase in a process for preparing a compound of formula (IIa). The invention further relates to the use of a hydrolase in a process for preparing a compound of formula (Ia).
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公开(公告)号：US11905538B2
公开(公告)日：2024-02-20
申请号：US17530018
申请日：2021-11-18
申请人： BASF SE
CPC分类号： C12P13/02 , A01N25/30 , A01N57/20 , C12N1/20 , C12N9/10 , C12P9/00 , C12P13/04 , C12P41/00 , C12Y104/03003 , C12Y206/01019 , A01N57/20 , A01N25/30 , A01N57/18
摘要： Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
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公开(公告)号：US20240018489A1
公开(公告)日：2024-01-18
申请号：US18333393
申请日：2023-06-12
申请人： Codexis, Inc.
发明人： Fabien Louis Cabirol , Haibin Chen , Anupam Gohel , Steven J. Collier , Derek J. Smith , Birgit Kosjek , Jacob Janey
CPC分类号： C12N9/0006 , C12Y101/01184 , C12P41/002 , C12P13/00 , C12P13/001 , C12P13/02 , Y02P20/52 , C12N15/70
摘要： The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
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公开(公告)号：US11732281B2
公开(公告)日：2023-08-22
申请号：US17464887
申请日：2021-09-02
申请人： BASF SE
CPC分类号： C12P13/02 , A01N25/30 , A01N57/20 , C12N1/20 , C12N9/10 , C12P9/00 , C12P13/04 , C12P41/00 , C12Y104/03003 , C12Y206/01019 , A01N57/20 , A01N25/30 , A01N57/18
摘要： Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
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公开(公告)号：US20210395790A1
公开(公告)日：2021-12-23
申请号：US17464887
申请日：2021-09-02
申请人： BASF SE
摘要： Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
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公开(公告)号：US20210254119A1
公开(公告)日：2021-08-19
申请号：US17239953
申请日：2021-04-26
申请人： Codexis, Inc.
发明人： Jeffrey C. Moore , Jack Liang , Jonathan Penfield , Jovana Nazor , Nikki Dellas , Vesna Mitchell , Da Duan , Iman Farasat , Agustina Rodriguez-Granillo , Grant Murphy , Nicholas Marshall
摘要： The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.
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公开(公告)号：US11008594B2
公开(公告)日：2021-05-18
申请号：US16087402
申请日：2017-03-21
发明人： Irén Hortobágyi , István Lászlófi , Zsuzsanna Kardos , József Molnár , László Takács , Tamás Bán
摘要： The invention provides a new enzimatic process for the preparation of chiral carboxylic acids, their salts and acid derivatives of the general formula (I) by enzymatic hydrolysis of racemic carboxylic acid ester of the general formula (II) and optionally subsequent esterification or acylation.
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公开(公告)号：US10995323B2
公开(公告)日：2021-05-04
申请号：US16717596
申请日：2019-12-17
申请人： Codexis Inc.
发明人： Ish K. Dhawan , Gregory Miller , Xiyun Zhang
摘要： The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
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公开(公告)号：US10988739B2
公开(公告)日：2021-04-27
申请号：US16708109
申请日：2019-12-09
申请人： Codexis, Inc.
发明人： Emily Mundorff , Erik Jan De Vries
IPC分类号： C12N15/00 , C12N15/53 , C07H21/04 , C12N9/04 , C12P17/14 , C12P41/00 , C12P7/02 , C12P17/10 , C12N15/75
摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
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