公开(公告)号：US20240117387A1

公开(公告)日：2024-04-11

申请号：US18541098

申请日：2023-12-15

申请人： Jiangnan University

发明人： Jingwen Zhou ,  Shan Li ,  Song Gao ,  Sha Xu ,  Weizhu Zeng ,  Shiqin Yu

IPC分类号： C12P7/02 ,  C12N9/10 ,  C12N9/88 ,  C12N15/81 ,  C12P5/00

CPC分类号： C12P7/02 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/81 ,  C12P5/002 ,  C12Y205/01 ,  C12Y402/03028 ,  C12N2800/102

摘要： The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

公开(公告)号：US20230212616A1

公开(公告)日：2023-07-06

申请号：US17625395

申请日：2019-07-08

申请人： SYMRISE AG

发明人： Dirk FABRITIUS ,  Stefan LAMBRECHT ,  Johannes PANTEN ,  Marcus EH

IPC分类号： C12P7/02 ,  C12P7/26 ,  C12N9/04

CPC分类号： C12P7/02 ,  C12P7/26 ,  C12N9/0006 ,  C12Y101/0105

摘要： The present invention relates to a method for the stereo selective production of a trans-diol or a cis-diol or a hydroxyketone comprising the step(s) (i) conversion of a trans-diol or a cis-diol to a hydroxyketone and/or (ii) conversion of a hydroxyketone to a cis-diol or a trans-diol, catalyzed by an enzyme, which is encoded by a nucleic acid sequence of SEQ ID NO: or wherein the enzyme comprises an amino acid sequence of SEQ ID NO: 2. The present invention also relates to the use of an enzyme encoded by a nucleic acid sequence of SEQ ID NO: 1 or wherein the enzyme comprises an amino acid sequence of SEQ ID NO: 2 for the conversion of a trans-diol to a cis-diol or for the conversion of a trans-diol or a cis-diol to a hydroxyketone and/or the conversion of a hydroxyketone to a trans-diol or a cis-diol.

公开(公告)号：US11618906B2

公开(公告)日：2023-04-04

申请号：US17055353

申请日：2019-05-14

申请人： HYPHA DISCOVERY LTD.

发明人： Jonathan Charles Paul Steele ,  Antonio De Riso ,  Francesco Falcioni ,  Richard Kerry Phipps ,  Stephen Keith Wrigley ,  Kinga Linda Nytko ,  Vincent Poon ,  Sebastian Schulz ,  John Maxim Ward ,  Mariacristina Bawn

IPC分类号： C12N1/20 ,  C12P7/66 ,  C12P7/02 ,  C12P17/16 ,  C12P17/18

摘要： The use of a cytochrome P-450 enzyme comprising SEQ ID NO: 110, or a variant enzyme having at least 70% identity thereto and having CYP-450 activity, for the hydroxylation of an organic compound, wherein the amino acid residue at position 291 is not threonine.

公开(公告)号：US11584941B2

公开(公告)日：2023-02-21

申请号：US16643740

申请日：2018-09-01

申请人： ISOBIONICS B.V.

发明人： Martinus Julius Beekwilder ,  Adèle Margaretha Maria Liduina Van Houwelingen ,  Hendrik Jan Bosch ,  Georg Friedrich Lentzen ,  Elena Melillo

IPC分类号： C12N15/63 ,  C12P7/02 ,  C12N9/88 ,  C12N15/80

摘要： The invention is directed to a patchoulol synthase, to a nucleic acid encoding said patchoulol synthase, to an expression vector comprising said nucleic acid, to a host cell comprising said expression vector, to a method of preparing patchoulol and elemol, and preferably also pogostol, and to a method of preparing a patchoulol synthase.

公开(公告)号：US11512272B2

公开(公告)日：2022-11-29

申请号：US16312238

申请日：2017-06-21

申请人： Qvidja Kraft AB

发明人： Anni Alitalo ,  Marko Niskanen ,  Erkki Aura

IPC分类号： C12M1/107 ,  C12N11/14 ,  C12M1/04 ,  C12P1/04 ,  C12N1/20 ,  C12P7/02 ,  C12P7/40 ,  C12M1/16 ,  C12M1/12 ,  C12P5/02

摘要： An active solid state fermentation bioreactor for producing gases, liquid(s) or solids from gaseous or gaseous and liquid starting materials and a fermentation process using the reactor are disclosed, The bioreactor includes three major phases; a solid phase including the porous solid support, a liquid phase comprising liquid, and a gaseous phase. The solid phase includes a porous solid support, in which at least 20% of the pore volumes have a size resulting in a liquid suction of about 0.01 to about 0.1 bars if these pores are filled with liquid, the porous solid support is inoculated with desired micro-organisms, the volume of the gaseous phase is 20% to 60% of the volume of the bioreactor, and the liquid phase is at least 20% of the reactor volume, The unsaturated capillary conductivity of filling/packing solid material of the bioreactor is at least 0.1 cm/ h. The solid state fermentation bioreactor enables a large gas-liquid interface, in which the filling material has a good capillary conductivity despite the unsaturated state.

公开(公告)号：US11377675B2

公开(公告)日：2022-07-05

申请号：US16744869

申请日：2020-01-16

申请人： Kuehnle AgroSystems, Inc.

发明人： Adelheid R. Kuehnle ,  Robert J. Schurr ,  Michael C. Perez ,  Norie Anne B. Nolasco

IPC分类号： C12P19/04 ,  C12P17/16 ,  C12P5/02 ,  C12P7/02 ,  A23K10/12 ,  A23L17/60 ,  A61K31/01 ,  A61K31/045 ,  A61K31/409 ,  A61K47/26 ,  A61K8/31 ,  A61K8/34 ,  A61Q19/00 ,  A61K8/73 ,  A61K8/49 ,  A61K9/00

摘要： The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell obtained from a subterranean habitat for producing the product of interest. The microalgal cell obtained from a subterranean habitat can be cultured in the dark, in light, in low nutrition, or nutrient rich conditions for at least a portion of production cycle. A combination of these conditions can be used to specifically manipulate a microalgal cell culture to produce a product of interest. The product of interest can be a water-soluble carotenoid, for example, a water-soluble carotenoid produced by culturing an algae belonging to the genus Haematococcus or a capsular exopolysaccharide produced by culturing an algae belonging to the genus Parachlorella. Compositions containing the water-soluble carotenoid, for example, as sunscreen and compositions containing the exopolysaccharide, for example, as moisturizing cream are also described.

公开(公告)号：US20210340039A1

公开(公告)日：2021-11-04

申请号：US17274308

申请日：2019-09-12

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT NATIONAL POLYTECHNIQUE DE TOULOUSE ,  lNSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA) ,  SUEZ Groupe ,  lNSTITUT NATIONAL DE RECHERCHE EN SCIENCES ET TECHNOLOGIES POUR L'ENVIRONNEMENT ET L'AGRICULTURE (IR

发明人： Sylvain MOREAU ,  Théodore BOUCHEZ ,  Jianghao TIAN ,  Elie LE QUEMENER ,  Alain BERGEL ,  Elise BLANCHET ,  Benjamin ERABLE ,  Luc ETCHEVERRY ,  Alain HUYARD ,  Pierre MAURICRACE ,  Nicolas BERNET ,  Eric TRABLY

IPC分类号： C02F3/00 ,  C12P7/02 ,  C25B3/25 ,  C25B11/043 ,  C25B9/63 ,  C25B3/23

摘要： A bioelectrochemical reactor (1) has an anode chamber (11) having at least two bioanodes (12, 13), and an anodic electrolyte (14) with an anodic electroactive microorganisms,—a cathode chamber (21) with at least one biocathode (22), and a cathodic electrolyte (24) with a cathodic electroactive microorganisms. The anode chamber (11) is separated from the cathode chamber (21) by, running from the anode chamber to the cathode chamber, a cation exchange membrane (31) and an anion exchange membrane (32). The cation and anion exchange membranes are separated from each other by an inter-membrane chamber (30), and means for applying a potential difference between the interconnected bioanodes and the biocathode/biocathodes. The bioanodes and biocathode/biocathodes have active surfaces such that the total active surface of the biocathode/biocathodes (22) is greater than the total active surface of the two bioanodes (12, 13). The arrangement includes a method for regenerating the activity of the bioanodes of the reactor and to the use of said reactor for the electrosynthesis of organic acids and/or alcohols from organic waste.

公开(公告)号：US11091781B2

公开(公告)日：2021-08-17

申请号：US16793577

申请日：2020-02-18

申请人： President and Fellows of Harvard College

发明人： Brendan Cruz Colón ,  Chong Liu ,  Marika Ziesack ,  Pamela Ann Silver ,  Daniel G. Nocera

IPC分类号： C12P7/02 ,  C25B11/04 ,  C25B15/08 ,  C25B3/04 ,  C25B1/04 ,  C25B3/25

摘要： Systems and methods for fixing carbon using bacteria are described. In one embodiment, a system includes a reactor chamber with a solution contained therein. The solution may include hydrogen (H2), carbon dioxide (CO2), bioavailable nitrogen, and a chemolithoautotrophic bacteria. The system may also include a pair of electrodes that split water contained within the solution to form the hydrogen. Additionally, the system may be operated so that a concentration of the bioavailable nitrogen in the solution is below a threshold nitrogen concentration to cause the chemolithoautotrophic bacteria to produce a product.

公开(公告)号：US20210147880A1

公开(公告)日：2021-05-20

申请号：US16969965

申请日：2019-02-14

申请人： Ginkgo Bioworks, Inc.

发明人： Christian Ridley ,  Jue Wang ,  Scott Marr

IPC分类号： C12P5/00 ,  C12N9/88 ,  C12P7/02

摘要： Described herein are chimeric terpene synthases, methods for making chimeric terpene synthases, and methods for making terpenes using the same.

公开(公告)号：US10941427B2

公开(公告)日：2021-03-09

申请号：US15675068

申请日：2017-08-11

申请人： AJINOMOTO CO., INC. ,  TOKYO INSTITUTE OF TECHNOLOGY

发明人： Mitsuhiro Kishino ,  Hiroyuki Kojima ,  Hideo Hosono ,  Michikazu Hara ,  Masaaki Kitano ,  Toshiharu Yokoyama ,  Toru Numaguchi ,  Munenobu Ito ,  Kazuteru Yamada ,  Hiromi Noguchi

IPC分类号： C12P13/04 ,  C12P13/08 ,  C12P13/14 ,  C12P1/00 ,  C12P7/02 ,  C12P7/40 ,  C12P19/04 ,  C12P21/00 ,  B01J21/00 ,  B01J23/02 ,  B01J23/46 ,  B01J27/24 ,  B01J35/00 ,  B01J37/04 ,  B01J37/08 ,  C07C273/00 ,  C01C1/04 ,  C01C1/18 ,  C01C1/28 ,  C01B21/38 ,  C12M1/00 ,  C12N1/00 ,  C07C273/02 ,  B01J21/06

摘要： A production system for a product selected from a nitrogen-containing product and a fermented and cultured product that does not involve (or can minimize) the transport of liquid ammonia can include: an ammonia synthesis apparatus in which an ammonia-containing gas is synthesized by reaction of a source gas containing hydrogen and nitrogen in the presence of a supported metal catalyst containing as a support one or more selected from the group consisting of: i) a conductive mayenite compound; ii) a two-dimensional electride compound or a precursor thereof; and iii) a complex formed of a support base containing at least one metal oxide selected from ZrO2, TiO2, CeO2, and MgO and a metal amide represented by a formula M(NH2)x (where M represents one or more selected from Li, Na, K, Be, Mg, Ca, Sr, Ba, and Eu; and x represents a valence number of M) supported by the support base.