公开(公告)号：US12123038B2

公开(公告)日：2024-10-22

申请号：US16284941

申请日：2019-02-25

申请人： National Technology & Engineering Solutions of Sandia, LLC

发明人： Parthasarathi Ramakrishnan ,  Jian Sun ,  Tanmoy Dutta ,  Blake A. Simmons ,  Seema Singh

IPC分类号： C12P19/02 ,  C08F251/02 ,  C08L97/00 ,  C12P7/10 ,  C12P19/14

CPC分类号： C12P19/02 ,  C08F251/02 ,  C08L97/00 ,  C12P7/10 ,  C12P19/14 ,  C12P2201/00

摘要： The present invention provides for novel protic ionic liquids (PIL) or phosphate-based ionic liquid (PBIL) useful for lignocellulosic processing described herein. The novel protic ionic liquids are capable of pretreatment of lignocellulosic biomass over a wide range of pH.

公开(公告)号：US12098409B2

公开(公告)日：2024-09-24

申请号：US17983665

申请日：2022-11-09

申请人： TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED

发明人： Yechezkel Kashi ,  Inbar Kesten ,  Keren Buhnik-Rosenblau

IPC分类号： C12P7/10 ,  C12N1/16 ,  C12N1/18 ,  C12N1/20 ,  C12N9/28 ,  C12N9/34 ,  C12N9/42 ,  C12N9/54 ,  C12P7/06 ,  C12R1/07 ,  C12R1/38 ,  C12R1/84 ,  C12R1/85

CPC分类号： C12P7/10 ,  C12N9/2417 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/54 ,  C12P7/065 ,  C12N1/165 ,  C12N1/185 ,  C12N1/205 ,  C12P2203/00 ,  C12R2001/07 ,  C12R2001/38 ,  C12R2001/84 ,  C12R2001/85 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01004

摘要： The present invention is directed to compositions comprising hydrolase-secreting bacteria and fermenting microorganisms and use thereof, such as for fermentative production of ethanol.

公开(公告)号：US12037371B2

公开(公告)日：2024-07-16

申请号：US17321941

申请日：2021-05-17

申请人： The Regents of the University of California

发明人： Michelle O'Malley ,  Kevin Solomon ,  Charles Haitjema

IPC分类号： C12P21/06 ,  C07K14/37 ,  C12N9/00 ,  C12N9/24 ,  C12N15/52 ,  C12P7/10 ,  C12P21/02 ,  C12P39/00 ,  C12N9/04 ,  C12N15/70 ,  C12N15/81

CPC分类号： C07K14/37 ,  C12N9/00 ,  C12N9/24 ,  C12N15/52 ,  C12P7/10 ,  C12P21/02 ,  C12P39/00 ,  C12N9/0006 ,  C12N15/70 ,  C12N15/81

摘要： Provided herein are novel proteins and protein domains from newly discovered anaerobic fungal species. The anaerobic fungal species have unique enzymatic capabilities, including the ability to digest diverse lignocellulosic biomass feedstocks and to synthesize secondary metabolites. The scope of the invention encompasses novel engineered proteins comprising glycoside hydrolase enzymes, dockerin domains, carbohydrate binding domains, and polyketide synthase enzymes. The invention further encompasses artificial cellulosomes comprising novel proteins and domains of the invention. The scope of the invention further includes novel nucleic acid sequences coding for the engineered proteins of the invention, and methods of using such engineered organisms to degrade lignocellulosic biomass and to create polyketides.

公开(公告)号：US20240226841A9

公开(公告)日：2024-07-11

申请号：US17769388

申请日：2016-06-13

申请人： Circ, LLC

发明人： Sandeep Kumar ,  Peter Majeranowski ,  Igor Kostenyuk ,  Iulian Bobe ,  Florin Barla

IPC分类号： B01J19/00 ,  B01D11/02 ,  C10G1/04 ,  C10G1/06 ,  C12M1/00 ,  C12P7/10 ,  C12P7/649 ,  D21B1/14 ,  D21C1/02 ,  D21C3/02 ,  D21C3/22 ,  D21C5/02 ,  D21C7/00 ,  D21C11/00 ,  D21H11/02

CPC分类号： B01J19/0053 ,  B01D11/0207 ,  C10G1/04 ,  C10G1/065 ,  C12M21/12 ,  C12M43/02 ,  C12P7/10 ,  C12P7/649 ,  D21B1/14 ,  D21C1/02 ,  D21C3/02 ,  D21C3/22 ,  D21C5/02 ,  D21C7/00 ,  D21C11/0007 ,  D21H11/02 ,  B01J2219/00051 ,  B01J2219/00162 ,  B01J2219/00353 ,  B01J2219/00891 ,  C10G2300/1014 ,  Y02E50/10 ,  Y02P30/20 ,  Y02W30/64

摘要： The presently disclosed subject matter relates to an industrial system for processing various plant materials to produce marketable materials. Particularly, the system integrates subcritical water extraction technology and includes a pre-processing module and a two-stage extractor (processing module) with constant control of temperature, pressure, and/or residence time. In some embodiments, the final product of the disclosed system can include feedstock constituents for biofuel production (sugars and/or oil), biochar, raw materials for various industries (such as pulp for manufacturing paper or cellulose for use in various industries). The disclosed system can be modular or non-modular, stationary or mobile, and can include prefabricated elements with programmed automatic or manual operation so that it can be easily moved and/or assembled on site.

公开(公告)号：US20240218401A1

公开(公告)日：2024-07-04

申请号：US18544037

申请日：2023-12-18

申请人： DANISCO US INC.

发明人： Paulus Petrus DE WAAL ,  Ingrid Maria VUGT-VAN LUTZ ,  Jozef Petrus Johannes SCHMITZ ,  Hans Marinus Charles Johannes DE BRUIJN

IPC分类号： C12P7/10 ,  C07K14/395 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10

CPC分类号： C12P7/10 ,  C07K14/395 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12Y101/01006 ,  C12Y102/0101 ,  C12Y203/01054 ,  C12P2203/00

摘要： The invention relates to a process for the production of ethanol from a composition comprising at least glucose comprising fermenting said composition in the presence of a recombinant yeast; and recovering the ethanol, wherein said yeast comprises one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding for an enzyme having dihydroxyacetone kinase activity (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter, wherein the composition comprises an amount of undissociated acetic acid of 10 mM or less. A recombinant yeast having the genes as described above is particularly sensitive towards acetic acid, and the ethanol yield rapidly decreases when the composition contains more than 10 mM undissociated acetic acid.

公开(公告)号：US12012608B2

公开(公告)日：2024-06-18

申请号：US18046988

申请日：2022-10-17

申请人： KWS SAAT SE & Co. KGaA

发明人： Karl Pioch ,  Wolfgang Koch ,  Stefan Meldau

IPC分类号： C12N15/82 ,  C12N9/44 ,  C12P5/02 ,  C12P7/10

CPC分类号： C12N15/8261 ,  C12N9/2451 ,  C12N15/8273 ,  C12P5/023 ,  C12P7/10 ,  C12Y302/01026

摘要： The present invention is directed to a transgenic maize plant or a part thereof comprising as transgene a nucleic acid capable of expressing a cell wall invertase or a functional part thereof, preferably a Chenopodium rubrum cell wall invertase or a functional part thereof, wherein as a result of the expression of the cell wall invertase or a functional part thereof the transgenic maize plant exhibits an enhanced tolerance to abiotic stress and/or an increased yield, to a method of producing such transgenic maize plant, to method of enhancing the tolerance to abiotic stress of a maize plant and/or of increasing yield potential of a maize plant, to a nucleic acid capable of expressing a cell wall invertase or a functional part thereof, preferably a Chenopodium rubrum cell wall invertase or a functional part thereof, to a vector comprising such nucleic acid, the use of the nucleic acid or vector for enhancing the tolerance to abiotic stress of a maize plant, for increasing yield potential of a maize plant and/or for protecting a maize plant against abiotic stress, and to a method for production of ethanol or methane from transgenic maize plant or a part thereof of the invention.

公开(公告)号：US11999985B2

公开(公告)日：2024-06-04

申请号：US17957633

申请日：2022-09-30

申请人： Edeniq, Inc.

发明人： Deepak Singh ,  Sandra Jacobson ,  Kris Ramos ,  Prachand Shrestha

IPC分类号： C12P19/14 ,  C07H3/04 ,  C12P7/10 ,  C12P19/02 ,  C12P19/12 ,  C13K1/02 ,  C13K13/00

CPC分类号： C12P19/14 ,  C07H3/04 ,  C12P7/10 ,  C12P19/02 ,  C12P19/12 ,  C13K1/02 ,  C13K13/002 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/10

摘要： The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.

公开(公告)号：US20240132926A1

公开(公告)日：2024-04-25

申请号：US18540631

申请日：2023-12-14

申请人： Cotton Incorporated

发明人： Matthew J. FARRELL ,  Sha FU ,  Mary A. ANKENY

IPC分类号： C12P19/02 ,  C12P7/10

CPC分类号： C12P19/02 ,  C12P7/10

摘要： Cotton-containing textiles, such as “trash” feedstock in terms of end-of-life-cotton textiles, may be used to produce sugar without the same kinds of harsh pretreatments used for other biomasses, such as corn, grass sources, or wood. Disclosed is a process for production of sugar from a cotton-containing textile waste fabric comprising optionally mechanically pretreating the cotton-containing textile, pretreating the cotton-containing textile with an acid pretreatment to form a slurry, cooling the slurry, adding at least one base to the slurry, adding at least one additional acid to the slurry to form a buffer in situ, adding a hydrolysis enzyme, and optionally filtering the slurry.

公开(公告)号：US20240052376A1

公开(公告)日：2024-02-15

申请号：US18256001

申请日：2021-12-09

申请人： Sekab E-Technology AB

发明人： Adnan Cavka ,  Elias Sundvall

IPC分类号： C12P7/10 ,  C12M1/33 ,  C12P19/14 ,  C12M1/34 ,  C08H8/00

CPC分类号： C12P7/10 ,  C12M45/02 ,  C12P19/14 ,  C12M41/12 ,  C08H8/00 ,  C12P2201/00 ,  C12P2203/00

摘要： The present disclosure generally relates to a method and a system for controlling the pretreatment and/or enzymatic hydrolysis of a lignocellulosic material. The method and system allow for savings with respect to energy consumption and costs.

公开(公告)号：US11899416B2

公开(公告)日：2024-02-13

申请号：US17392600

申请日：2021-08-03

申请人： MARATHON PETROLEUM COMPANY LP

发明人： David Whikehart ,  Gregory Herold

IPC分类号： C10G9/00 ,  C10G47/36 ,  C10L1/02 ,  C10L1/04 ,  C10L3/06 ,  C12P7/06 ,  E21B43/12 ,  E21B43/25 ,  G01N33/28 ,  G06Q50/06 ,  G06Q50/30 ,  G08C17/02 ,  C12P7/10 ,  C12P19/02 ,  G05B13/04 ,  C12M1/00 ,  C12M1/36 ,  G05B19/418 ,  G01N33/22 ,  G06Q30/018 ,  G06Q10/083

CPC分类号： G05B13/042 ,  C10G9/00 ,  C10G47/36 ,  C10L1/02 ,  C10L1/04 ,  C10L3/06 ,  C12M21/12 ,  C12M41/48 ,  C12P7/06 ,  E21B43/12 ,  E21B43/25 ,  G01N33/225 ,  G01N33/28 ,  G05B19/4189 ,  G06Q30/018 ,  G06Q50/06 ,  G06Q50/30 ,  G08C17/02 ,  C10G2300/1011 ,  C10G2300/4043 ,  C10L2200/0469 ,  C10L2200/0476 ,  C10L2230/14 ,  C10L2270/10 ,  C10L2290/24 ,  C10L2290/58 ,  G05B2219/45076 ,  G06Q10/083 ,  Y02P20/151

摘要： Systems and methods to provide low carbon intensity (CI) hydrogen through one or more targeted reductions of carbon emissions based upon an analysis of carbon emissions associated with a combination of various options for feedstock procurement, feedstock refining, processing, or transformation, and hydrogen distribution pathways to end users. Such options are selected to maintain the total CI (carbon emissions per unit energy) of the hydrogen below a pre-selected threshold that defines an upper limit of CI for the hydrogen.