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    Strategies for high throughput identification and detection of polymorphisms
    2.
    发明授权
    Strategies for high throughput identification and detection of polymorphisms 有权

    公开(公告)号：US10978175B2

    公开(公告)日：2021-04-13

    申请号：US16517411

    申请日：2019-07-19

    申请人： KEYGENE N.V.

    发明人： Michael Josephus Theresia Van Eijk ,  Henricus Johannes Adam Van Der Poel

    IPC分类号： G16B30/00 ,  C40B30/04 ,  C12Q1/6827 ,  C12N15/10 ,  C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6883 ,  C12Q1/6858 ,  C40B30/10

    摘要： The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.

    Rapid and sensitive method of forensic toxicology in post-mortem subjects and in live and post-mortem animals using oral fluid testing
    3.
    发明授权
    Rapid and sensitive method of forensic toxicology in post-mortem subjects and in live and post-mortem animals using oral fluid testing 有权

    公开(公告)号：US10753952B2

    公开(公告)日：2020-08-25

    申请号：US16390884

    申请日：2019-04-22

    申请人： Amy J. Reisinger

    发明人： Amy J. Reisinger

    IPC分类号： G01N33/94 ,  G01N30/72 ,  C40B30/10 ,  G01N30/04 ,  A61B10/00

    摘要： The present invention provides a rapid, sensitive method for forensic drug testing in a post-mortem subject or a live or a post-mortem animal using oral fluid collected from the post-mortem subject or live or post-mortem animal. The method comprises collecting a sample of oral fluid from a post-mortem subject or a live or a post-mortem animal, analyzing the oral fluid sample qualitatively to detect the presence of one or more non-naturally occurring drugs, analyzing the oral fluid sample quantitatively to determine concentration of the one or more non-naturally occurring drugs in the post-mortem subject or in the live or the post-mortem animal, and identifying the one or more non-naturally occurring drugs in the post-mortem subject or in the live or the post-mortem animal. The detection and quantification in oral fluid is more sensitive and faster than detection and quantification of the non-naturally occurring drugs in blood, urine, bile, and liver tissue collected from the same post-mortem subject. Further, the qualitative and quantitative results are obtained in as little as three hours.

    Sample mass spectrum analysis
    4.
    发明授权
    Sample mass spectrum analysis 有权

    公开(公告)号：US10714210B2

    公开(公告)日：2020-07-14

    申请号：US15578076

    申请日：2016-05-27

    申请人： Micromass UK Limited

    发明人： Keith George Richardson ,  Steven Derek Pringle

    IPC分类号： G16C20/20 ,  G01N30/72 ,  C40B30/10 ,  H01J49/16

    摘要： A method of analysing a sample mass spectrum comprises comparing a sample mass spectrum of a sample with each reference mass spectrum of plural reference mass spectra. A similarity index is assigned to each reference mass spectrum of the plural reference mass spectra based on similarity between the sample mass spectrum and the reference mass spectrum. For each group of one or more groups of the plural reference mass spectra, the similarity indexes for the reference mass spectra belonging to the group are combined so as to provide a group index for the group at a first level of a hierarchy of sample characteristics. The reference mass spectra belonging to each group are mass spectra of reference samples that have a particular characteristic in common. The method provides a way to categorise a sample as belonging to a particular group of reference samples.

    METHOD FOR DETERMINING AN ALLELE PROFILE OF NUCLEIC ACID
    10.
    发明申请
    METHOD FOR DETERMINING AN ALLELE PROFILE OF NUCLEIC ACID 有权
    用于确定核酸的等位基因的方法

    公开(公告)号：US20130029857A1

    公开(公告)日：2013-01-31

    申请号：US13190147

    申请日：2011-07-25

    申请人： Michael Seul

    发明人： Michael Seul

    IPC分类号： C40B30/04 ,  C40B30/10 ,  C07H21/00 ,  C40B30/00

    CPC分类号： C12Q1/6858 ,  G06F19/20 ,  C12Q2535/125 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2563/179

    摘要： A method of identifying alleles of polymorphic sites in a plurality of nucleic acid samples including the steps of determining a source tag sharing number “d” for each of the alleles; performing a first reaction in a plurality of pools of the alleles to be identified to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products to identify the alleles. If “d” is equal to or larger than a maximum pool size, the first reaction may not be performed. Alleles may be binned together. A microparticle comprising one or more capture probes each comprising an oligonucleotide complementary to a subsequence of a target polynucleotide.

    摘要翻译： 一种确定多个核酸样品中多态性位点的等位基因的方法，包括以下步骤：确定每个等位基因的源标签共享数d; 在要识别的等位基因的多个池中进行第一反应以产生包括识别所述每个池的源标签的反应产物; 集中池塘提供池池; 对于待鉴定的每个等位基因，使用所述反应产物进行第二反应以产生包含标记标签和衍生源标签的等位基因特异性第二反应产物; 识别所述等位基因特异性第二反应产物以鉴定等位基因。 如果d等于或大于最大池大小，则可能不执行第一反应。 等位基因可以合并在一起。 一种包含一种或多种捕获探针的微粒，每个捕获探针包含与靶多核苷酸的亚序列互补的寡核苷酸。