公开(公告)号：US12091776B2

公开(公告)日：2024-09-17

申请号：US16913572

申请日：2020-06-26

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： Harry B. Larman ,  Gabriel Roman-Melendez ,  Thiagarajan Venkataraman

IPC分类号： C40B40/08 ,  C07K17/06 ,  C12N7/00 ,  C12N15/63 ,  C12Q1/6869

CPC分类号： C40B40/08 ,  C07K17/06 ,  C12N7/00 ,  C12N15/63 ,  C12Q1/6869 ,  C07K2317/34 ,  C07K2319/43

摘要： The present invention relates to the field of proteases. More specifically, the present invention provides compositions and methods useful for profiling protease activity using phage display. In one embodiment, a display vector useful for profiling protease activity comprises a nucleic acid sequence encoding (a) a peptide to be displayed on the surface of the vector; (b) a first affinity tag C-terminal to the peptide; and (c) a second affinity tag N-terminal to the peptide. The display vector can comprise a virus, bacteriophage, yeast, bacteria, retrovirus, ribosome or mRNA. In particular embodiments, the peptide comprises a human peptidome library peptide.

公开(公告)号：US20240240360A1

公开(公告)日：2024-07-18

申请号：US18592456

申请日：2024-02-29

申请人： RULES-BASED MEDICINE INC.

发明人： Andrew Raymon Morton BRADBURY ,  Michael Frank ERASMUS ,  Andre TEIXEIRA

IPC分类号： C40B40/10 ,  C12N15/10 ,  C12N15/81 ,  C40B40/08 ,  C40B50/06

CPC分类号： C40B40/10 ,  C12N15/1037 ,  C12N15/81 ,  C40B40/08 ,  C40B50/06

摘要： Antibody libraries comprising a plurality of heavy chain variable domains and/or a plurality of light chain variable domains, which comprise complementary determining regions (CDRs) found in naturally-occurring human antibodies, and methods of making such antibody libraries. The antibody libraries are free of members that comprise one or more liabilities affecting one or more features of such members. Further, the antibody libraries comprise members having heavy chain and/or light chain CDRs not found in the same naturally-occurring human antibody.

公开(公告)号：US20240240359A1

公开(公告)日：2024-07-18

申请号：US18268538

申请日：2021-12-22

申请人： THE REGENTS OF THE UNIVERSITY OF MICHIGAN

发明人： Alison R. H. Narayan

IPC分类号： C40B40/08 ,  C12P1/00

CPC分类号： C40B40/08 ,  C12P1/00

摘要： Disclosed herein are methods for synthesizing or functionalizing organic compounds using a library of biocatalysts. The methods include separately admixing a reactant and an aqueous solvent with each biocatalyst in a library of biocatalysts to provide a library of product admixtures, wherein the admixing occurs under sustainable reaction conditions.

公开(公告)号：US20240200131A1

公开(公告)日：2024-06-20

申请号：US18415344

申请日：2024-01-17

申请人： Fred Hutchinson Cancer Center

发明人： Jason H. BIELAS

IPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12Q1/6827 ,  C12Q1/6869 ,  C40B40/08 ,  C40B50/06

CPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12Q1/6827 ,  C12Q1/6869 ,  C40B40/08 ,  C40B50/06

摘要： The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

公开(公告)号：US11987904B2

公开(公告)日：2024-05-21

申请号：US16081635

申请日：2017-03-02

申请人： Aptamer Group Limited

发明人： Shuji Ikeda ,  Shozo Fujita ,  Tsuyoshi Fujihara

IPC分类号： C40B40/06 ,  C12N15/10 ,  C12N15/115 ,  C40B40/08

CPC分类号： C40B40/08 ,  C12N15/1093 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/52 ,  C12N2320/11 ,  C12N2330/31

摘要： Oligonucleotide constructs are described, each comprising a functional element and a coding element, wherein the functional element comprises a functional sequence, the functional sequence comprising a sequence of nucleotides in which one or more, or each, nucleotide is modified and the coding element comprises a coding sequence, the coding sequence comprising a sequence of nucleotides which do not contain the modifications of the functional sequence, wherein the coding sequence encodes the sequence structure of the functional sequence.

公开(公告)号：US11970694B2

公开(公告)日：2024-04-30

申请号：US16807435

申请日：2020-03-03

申请人： PeptiDream Inc.

发明人： Kenji Kashiwagi ,  Patrick Reid

IPC分类号： C12N15/10 ,  C40B40/08 ,  C40B50/06

CPC分类号： C12N15/1062 ,  C40B40/08 ,  C40B50/06

摘要： Provided are linkers suitable for preparing a conjugate of a nucleic acid and a peptide as a translation product thereof in a reconstituted cell-free translation system in genotype-phenotype mapping (display methods), said linkers comprising a single-stranded structure region having a side chain base pairing with the base at the 3′-end of an mRNA at one end and a peptidyl acceptor region containing an amino acid attached to an oligo RNA consisting of a nucleotide sequence of ACCA via an ester bond at the other end, characterized in that the ester bond is formed by using an artificial RNA catalyst. Also provided are display methods using [mRNA]-[linker]-[peptide] conjugates assembled via such linkers.

公开(公告)号：US11939355B2

公开(公告)日：2024-03-26

申请号：US17209336

申请日：2021-03-23

申请人： Howard Hughes Medical Institute ,  The Regents of the University of California

发明人： Joshua Dudman ,  Adam Hantman ,  Bum-Yeol Hwang ,  Alla Karpova ,  Loren Looger ,  Kimberly Ritola ,  David Schaffer ,  Dougal Gowanlock Robinson Tervo ,  Sarada Viswanathan

IPC分类号： C12N7/00 ,  C07K14/005 ,  C07K14/015 ,  C12N15/86 ,  C40B30/06 ,  C40B40/08 ,  A61K48/00

CPC分类号： C07K14/015 ,  C07K14/005 ,  C12N15/86 ,  C40B30/06 ,  C40B40/08 ,  A61K48/00 ,  C12N2750/14122 ,  C12N2750/14145 ,  C12N2810/6027

摘要： The present disclosure provides AAV variants that exhibit a preference for retrograde movement in neurons and methods of using such variants.

公开(公告)号：US11802281B2

公开(公告)日：2023-10-31

申请号：US16090829

申请日：2017-03-17

申请人： ETH ZURICH

发明人： Sai Reddy ,  William Kelton ,  Cristina Parola ,  Derek Mason ,  Mark Pogson

IPC分类号： C40B20/04 ,  C12N15/10 ,  C12N5/0781 ,  C12N9/22 ,  C12N15/113 ,  C40B10/00 ,  C40B40/08

CPC分类号： C12N15/1037 ,  C12N5/0635 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1058 ,  C12N15/1082 ,  C12N15/1086 ,  C12N15/113 ,  C40B10/00 ,  C40B20/04 ,  C40B40/08 ,  C12N15/1034 ,  C12N2310/20 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2800/80 ,  C12Q2521/539 ,  C12N15/102 ,  C12Q2521/539

摘要： According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.

公开(公告)号：US20230340696A1

公开(公告)日：2023-10-26

申请号：US17963509

申请日：2022-10-11

申请人： F-STAR THERAPEUTICS LIMITED

发明人： Gottfried Himmler ,  Florian Ruker ,  Gordana Wozniak-Knopp

IPC分类号： C40B40/08 ,  C07K19/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/40

CPC分类号： C40B40/08 ,  C07K19/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/40 ,  C07K2317/526 ,  C40B40/10

摘要： Libraries of immunoglobulins which each have one or more amino acid modifications in at least one structural loop region of such immunoglobulins, where the modified loop region specifically binds to an epitope of an antigen to which an unmodified immunoglobulin does not significantly bind.

公开(公告)号：US11795479B2

公开(公告)日：2023-10-24

申请号：US17222936

申请日：2021-04-05

申请人： The Regents of the University of Colorado

发明人： Andrew Garst ,  Ryan T Gill

IPC分类号： C12N15/90 ,  C12N15/10 ,  C40B40/08 ,  C12N15/11 ,  C12N9/22

CPC分类号： C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/1082 ,  C12N15/1093 ,  C12N15/11 ,  C12N15/111 ,  C40B40/08 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2320/11 ,  C12N2320/12 ,  C12N2330/31

摘要： Described herein are method for generating a vector for editing a cell. The method comprises ligating into a vector that encodes a portion of a gRNA a cassette comprising at least one editing cassette, a promoter, and a gene encoding another portion of the gRNA. Upon ligation, the portion of the gRNA from the editing cassette and the other portion of the gRNA are ligated and form a functional gRNA.