Apparatus and method for single cell discrimination

    公开(公告)号:US11994484B2

    公开(公告)日:2024-05-28

    申请号:US17703783

    申请日:2022-03-24

    摘要: A method includes providing a fluid to a structure including an aperture, applying a voltage signal to a circuit that includes the fluid, applying a substantially periodic pressure signal to the fluid, detecting a current signal in the circuit as an analyte passes through the aperture in response to the substantially periodic pressure signal, and processing the current signal and the substantially periodic pressure signal to determine a switch time and a release time for the analyte. An apparatus includes a structure including an aperture to receive a fluid, a voltage source to provide a voltage signal to an electronic circuit having a path that includes the aperture, a pressure signal generator to provide a substantially periodic pressure signal to the fluid, and a system to process the periodic pressure signal and a current signal induced in the electronic circuit.

    METHOD FOR RNA MANUFACTURING
    4.
    发明公开

    公开(公告)号:US20240167068A1

    公开(公告)日:2024-05-23

    申请号:US18569372

    申请日:2022-06-30

    摘要: The present invention relates to the field of nucleic acid production, in particular in vitro RNA transcription. More specifically, the present invention relates to a method wherein physicochemical properties of the reaction mixture are monitored in order to timely stop the reaction and/or to add fresh reagents to allow the reaction to continue under optimal conditions. In particular, conductivity, but also pH and/or optical density is determined at at least two different time points, or continuously during the in vitro transcription reaction.

    CONDUCTIVITY MEASUREMENT DEVICE
    7.
    发明公开

    公开(公告)号:US20230349960A1

    公开(公告)日:2023-11-02

    申请号:US18213450

    申请日:2023-06-23

    申请人: Philip SALKIE

    发明人: Philip SALKIE

    IPC分类号: G01R27/02 G01N27/06 G01N27/07

    CPC分类号: G01R27/02 G01N27/06 G01N27/07

    摘要: In some embodiments, the conductivity measurement device includes a conductivity probe, a solid state switch device, and a DC measurement circuit. The conductivity probe includes a first and second measurement pin used to measure a conductivity of the liquid. The solid state switch device is coupled to the conductivity probe and is configured to connect and disconnect the first measurement pin and second measurement pin to a first DC reference voltage and a second DC reference voltage. The DC measurement circuit is configured to generate a measurement signal such that the measurement signal is maintained at a first DC reference voltage and the first DC reference voltage is applied to the solid state switch device from the DC measurement circuit. In this manner, an alternating current (AC) voltage is applied to the measurement pins utilizing DC reference voltages, which helps to avoid contamination of the liquid.

    SENSING DEVICE COMPENSATING FOR CROSS-SENSITIVITY TO ANALYTES

    公开(公告)号:US20230296545A1

    公开(公告)日:2023-09-21

    申请号:US17697515

    申请日:2022-03-17

    申请人: Robert Bosch GmbH

    IPC分类号: G01N27/06 B01L3/00 G01N21/64

    摘要: A sensing device including film sensor, a second sensor, and a controller. The film sensor includes a receptor capturing an intended analyte and an unintended analyte in a fluid medium to generate a film sensor signal. The receptor has a receptor cross-sensitivity to the unintended analyte. The second sensor contacts the fluid medium including the intended analyte and the unintended analyte to generate a second sensor signal. The second sensor has a second cross-sensitivity to the unintended analyte different than the receptor cross-sensitivity to the unintended analyte. The controller is programmed to determine a concentration of the intended analyte in the fluid medium in response to the film sensor signal, the receptor cross-sensitivity, the second sensor signal, and the second cross-sensitivity.