公开(公告)号：WO2022066104A1

公开(公告)日：2022-03-31

申请号：PCT/SG2021/050582

申请日：2021-09-24

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： BIN HAJIS, Muhammad Irfan ,  CHEN, Liwei ,  ONG, Ting Xun Nicholas ,  CHEW, Wei Leong

IPC: C12Q1/6876 ,  C12Q1/70

Abstract: Disclosed herein is a primer set for a nucleic acid amplification analysis to detect a SARS-CoV-2 virus, the primer set is selected from the group consisting of Set 12 against Orf1ab 2'-0-ribose methyltransferase: SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 7 attached to SEQ ID No. 8 by a first linker, and SEQ ID No. 9 attached to SEQ ID No. 10 by a second linker; Set 10 against orf3a: SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 17 attached to SEQ ID No. 18 by a third linker, and SEQ ID No. 19 attached to SEQ ID No. 20 by a fourth linker; Set 20 against S gene: SEQ ID No. 21, SEQ ID No. 22, SEQ ID No. 27 attached to SEQ ID No. 28 by a fifth linker, and SEQ ID No. 29 attached to SEQ ID No. 30 by a sixth linker; and any combinations thereof. In specific embodiments, the nucleic acid amplification analysis is reverse transcription-loop mediated isothermal amplification (RT-LAMP).