公开(公告)号：WO1997029635A1

公开(公告)日：1997-08-21

申请号：PCT/US1997002448

申请日：1997-02-14

Applicant: PICOWER INSTITUTE FOR MEDICAL RESEARCH

Inventor： PICOWER INSTITUTE FOR MEDICAL RESEARCH ,  BUCALA, Richard ,  ULRICH, Peter, C. ,  XHANG, Xini ,  CERAMI, Anthony ,  BENDRAT, Klaus

IPC: A01N43/34

CPC classification number: A61K31/404 ,  A61K38/00 ,  C07K14/52

Abstract: There is disclosed assays to identify compounds that inhibit an enzymatic activity of MIF (macrophage migration inhibitory factor). The enzyme activity catalyzes a tautomerization of MIF-substrates, such as D-dopachrome to DHICA. In general, the assay is conducted in vitro by adding, mixing or combining MIF polypeptide and a suitable substrate in the presence or absence of a test compound, and measuring the tautomerization of the substrate. The test compounds that inhibit tautomerization in the assay are identified as MIF inhibitors.

Abstract translation: 公开了鉴定抑制MIF的酶活性（巨噬细胞迁移抑制因子）的化合物的测定法。 酶活性催化MIF-底物的互变异构，例如D-多巴色素到DHICA。 通常，通过在存在或不存在测试化合物的情况下通过加入，混合或组合MIF多肽和合适的底物并测量底物的互变异构体进行测定。 在测定中抑制互变异构化的测试化合物被鉴定为MIF抑制剂。