公开(公告)号：WO2014105788A2

公开(公告)日：2014-07-03

申请号：PCT/US2013/077411

申请日：2013-12-23

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： BOTES, Adriana Leonora ,  CONRADIE, Alex Van Eck ,  CHEN, Changlin ,  PEARLMAN, Paul S.

IPC: C12P13/00 ,  C12P7/44 ,  C12P7/42 ,  C12P7/18

CPC classification number: C12P13/001 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/16 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P13/002 ,  C12Y102/99006 ,  C12Y301/02002

Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.

Abstract translation: 该文献描述了通过在C7脂肪族主链基质中形成由羧基，胺或羟基组成的两个末端官能团来生产庚二酸，7-氨基庚酸，7-羟基庚酸，七亚甲基二胺或1,7-庚二醇的生化途径。 本文描述的这些途径，代谢工程和培养策略依赖于与酸性营养不良食物或2-氨基己二酸赖氨酸生物合成的环己烷羧酸盐生物合成相关的碳链延长酶或其同系物。