公开(公告)号：WO2021183899A1

公开(公告)日：2021-09-16

申请号：PCT/US2021/022128

申请日：2021-03-12

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

Inventor： ENGELTHALER, David ,  BOWERS, Jolene ,  SCHUPP, James

IPC: C12P19/34 ,  C12Q1/68 ,  C12Q1/70 ,  C12Q15/10

Abstract: Methods, kits, and oligonucleotides used in the detection of coronavirus, for example, SARS-CoV-2, CoV-HKU1, CoV-OC43, CoV-229E, and CoV-NL63, are disclosed. A method of detecting coronavirus may include contacting a sample with at least one primer pair and probe targeting SARS-CoV-2 and at least one of: a primer pair and probe targeting CoV-HKU1, a primer pair and probe targeting CoV-OC43, a primer pair and probe targeting CoV-229E, a primer pair and probe targeting CoV-NL63, subjecting the mixture to conditions that allow nucleic acid amplification, and detecting the presence or absence of coronavirus, including SARS-CoV-2, CoV-HKU1, CoV-OC43, CoV-229E, and/or CoV-NL63, by analyzing the nucleic acid amplification products.

公开(公告)号：WO2018200887A1

公开(公告)日：2018-11-01

申请号：PCT/US2018/029666

申请日：2018-04-26

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY ,  CENTERS FOR DISEASE CONTROL AND PREVENTION (CDC)

Inventor： DRIEBE, Elizabeth ,  BOWERS, Jolene ,  ENGELTHALER, David ,  KEIM, Paul ,  LIMBAGO, Brandi ,  RASHEED, James, K. ,  MCDOUGAL, Linda ,  ALBRECHT, Valerie, S.

IPC: C12Q1/689 ,  C12Q1/6888 ,  C12Q1/6844 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12R1/445

Abstract: The present invention provides a method of detecting Staphylococcus aureus in a subject, by contacting a sample obtained from the subject with at least one detectably labeled probe of the invention or detecting in the sample identity to a sequence of the invention. The invention is also directed to kits, microarrays and detectable Staphylococcus aureus polynucleotide probes useful in detecting the presence of Staphylococcus aureus.

公开(公告)号：WO2017160779A3

公开(公告)日：2017-09-21

申请号：PCT/US2017/022211

申请日：2017-03-14

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： BOWERS, Jolene ,  DRIEBE, Elizabeth ,  ENGELTHALER, David ,  KEIM, Paul ,  LEMMER, Darrin

IPC: C12Q1/68 ,  C12Q1/70 ,  A61P31/10 ,  C12N1/14 ,  C12N1/20 ,  C12N15/11 ,  C12R1/01 ,  G06F19/10

Abstract: The present invention provides a method of detecting one or more Klebsiella species within a sample from a subject, the method comprising: subjecting DNA and/or RNA from the sample to a PCR amplification reaction using primer pairs targeting species-specific canonical single nucleotide polymorphisms (canSNPs); and analyzing amplification products resulting from the PCR amplification reaction to detect the one or more Klebsiella species. The present invention also provides a kit for detection of one or more Klebsiella species, Klebsiella clonal groups, AMR genes, and/or virulence genes, the kit comprising primer pairs targeting species-specific canSNPs, K. pneumoniae genes M1 and M2, clonal group-specific canSNPs, AMR genes, and/or virulence genes.

公开(公告)号：WO2017160779A2

公开(公告)日：2017-09-21

申请号：PCT/US2017/022211

申请日：2017-03-14

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： BOWERS, Jolene ,  DRIEBE, Elizabeth ,  ENGELTHALER, David ,  KEIM, Paul ,  LEMMER, Darrin

IPC: C12Q1/68

Abstract: The present invention provides a method of detecting one or more Klebsiella species within a sample from a subject, the method comprising: subjecting DNA and/or RNA from the sample to a PCR amplification reaction using primer pairs targeting species-specific canonical single nucleotide polymorphisms (canSNPs); and analyzing amplification products resulting from the PCR amplification reaction to detect the one or more Klebsiella species. The present invention also provides a kit for detection of one or more Klebsiella species, Klebsiella clonal groups, AMR genes, and/or virulence genes, the kit comprising primer pairs targeting species-specific canSNPs, K. pneumoniae genes M1 and M2, clonal group-specific canSNPs, AMR genes, and/or virulence genes.

Abstract translation: 本发明提供了检测来自受试者的样品中的一种或多种克雷伯菌属物种的方法，所述方法包括：使用靶向引物对将来自样品的DNA和/或RNA进行PCR扩增反应 物种特异性典型单核苷酸多态性（canSNP）; 并分析由PCR扩增反应产生的扩增产物以检测一种或多种克雷伯菌属物种。 本发明还提供了用于检测一种或多种克雷伯菌属物种，克雷伯氏菌克隆群，AMR基因和/或毒力基因的试剂盒，所述试剂盒包含靶向物种特异性canSNP，肺炎克雷伯氏菌基因M1和M2，克隆组 特异性canSNP，AMR基因和/或毒力基因。