公开(公告)号：WO1992019716A1

公开(公告)日：1992-11-12

申请号：PCT/DK1992000060

申请日：1992-02-27

Applicant: AGRO-FERM A/S ,  KIEL, Pauli ,  ANDERSEN, Margrethe

Inventor： AGRO-FERM A/S

IPC: C12N01/00

CPC classification number: C12P13/04 ,  C12N1/20 ,  C12P7/40

Abstract: The procedure involves heat treatment of the plant juice, first in a temperature x time interval from 55 DEG C x 24 hours to 120 DEG C x 10 min., whereafter the plant juice is cooled to 50 DEG C - 60 DEG C and the pH value is set at 7.5 - 8.5 by addition of a base. Hereafter the plant juice is converted by enzymic hydrolysis by means of proteolytic enzymes such as proteases and peptidases, while keeping the pH value constant at 7.5 - 8.5 by continuous addition of base until the hydrolysis is over. By the procedure a medium is formed which is well suited as a nutritive substrate for vitamin and amino acid demanding micro-organisms, which form organic acids or amino acids. By the procedure it is possible in a cheap and simple manner to convert plant juice to organic acids or amino acids.

Abstract translation: 该方法涉及植物汁的热处理，首先在55℃×24小时至120℃×10分钟的温度x时间间隔内，将植物汁冷却至50℃〜60℃， 通过添加基数将值设置为7.5-8.5。 此后，通过蛋白水解酶如蛋白酶和肽酶通过酶水解转化植物汁，同时通过连续加入碱保持pH值恒定在7.5-8.5，直到水解结束。 通过该程序，形成适合作为维生素和氨基酸要求微生物的营养底物的培养基，其形成有机酸或氨基酸。 通过该程序，可以以便宜和简单的方式将植物汁转化为有机酸或氨基酸。