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    SOLID PHASE SYNTHESIS OF ACRIDINIUM DERIVATIVES
    4.
    发明申请
    SOLID PHASE SYNTHESIS OF ACRIDINIUM DERIVATIVES 审中-公开
    茜素衍生物的固相合成

    公开(公告)号：WO2007092847A2

    公开(公告)日：2007-08-16

    申请号：PCT/US2007061696

    申请日：2007-02-06

    申请人： SIEMENS MEDICAL SOLUTIONS DIAG ,  NATRAJAN ANAND ,  SHARPE DAVID ,  COSTELLO JIM ,  JIANG QINGPING

    发明人： NATRAJAN ANAND ,  SHARPE DAVID ,  COSTELLO JIM ,  JIANG QINGPING

    CPC分类号： C07D219/04

    摘要： Acridinium-functionalized solid-phase supports and methods for making acridinium-functionalized solid-phase supports are disclosed. The acridinium-functionalized solid-phase supports comprise a solid phase support linked to a chemiluminescent substituted acridinium compound through a linker group covalently attached to the nitrogen atom of the acridinium nucleus and the solid phase support.

    摘要翻译： 公开了吖啶鎓官能化固相载体和制备吖啶酮官能化固相载体的方法。 吖啶酮官能化的固相载体包括通过共价连接到吖啶核的氮原子的连接基团和固相载体连接到化学发光的取代的吖啶鎓化合物的固相载体。

    CHEMILUMINESCENT SUBSTRATES OF HYDROLYTIC ENZYMES SUCH AS PHOSPHATASES
    8.
    发明申请
    CHEMILUMINESCENT SUBSTRATES OF HYDROLYTIC ENZYMES SUCH AS PHOSPHATASES 审中-公开
    水解酶如CHPHUMLESETIC基质如磷酸盐

    公开(公告)号：WO0109372B1

    公开(公告)日：2001-06-14

    申请号：PCT/US0020429

    申请日：2000-07-27

    申请人： BAYER AG ,  JIANG QINGPING ,  NATRAJAN ANAND ,  SHARPE DAVID J ,  WONG WEN JEE ,  LAW SAY JONG

    发明人： JIANG QINGPING ,  NATRAJAN ANAND ,  SHARPE DAVID J ,  WONG WEN-JEE ,  LAW SAY-JONG

    IPC分类号： G01N21/78 ,  C07D219/04 ,  C07D491/10 ,  C07F9/64 ,  C09K11/07 ,  C12M1/34 ,  C12Q1/34 ,  C12Q1/42 ,  C07D219/06

    CPC分类号： C07D491/10 ,  C07D219/04 ,  C07F9/64 ,  C12Q1/42 ,  C12Q2334/00

    摘要： Chemiluminescent substrates of hydrolytic enzymes are disclosed having general Formula Lumi-M-P, where Lumi is a chemiluminescent moiety capable of producing light (a) by itself, (b) with MP attached and (c) with M attached, wherein the different properties of Lumi-M-P and Lumi-M allow them to be distinguished. Lumi includes, but is not limited to, acridinium compounds (e.g. acridinium esters, carboxyamides, thioesters and oxime esters), reduced forms thereof (e.g. acridans), and spiroacridan compounds. M is selected from oxygen, nitrogen and sulfur. P is a group that can be readily removed by hydrolytic enzymes to give Lumi-M and P. The hydrolytic enzyme can be phosphatase, glycosidase, peptidase, protease, esterase, sulfatase and guanidinobenzoatase.

    摘要翻译： 公开了水解酶的化学发光底物，其具有通式Lumi-MP，其中Lumi是能够自身产生光（a）的化学发光部分，（b）MP附着和（c）与M连接，其中Lumi的不同性质 -MP和Lumi-M允许他们被区别。 Lumi包括但不限于吖啶鎓化合物（例如吖啶酯，羧酰胺，硫酯和肟酯），还原形式（例如吖啶）和螺吡啶化合物。 M选自氧，氮和硫。 P是可以通过水解酶容易地除去得到Lumi-M和P的基团。水解酶可以是磷酸酶，糖苷酶，肽酶，蛋白酶，酯酶，硫酸酯酶和胍基苯甲酰化酶。

    NEAR INFRARED CHEMILUMINESCENT ACRIDINIUM COMPOUNDS AND USES THEREOF
    9.
    发明申请
    NEAR INFRARED CHEMILUMINESCENT ACRIDINIUM COMPOUNDS AND USES THEREOF 审中-公开
    近红外化学发光吖啶化合物及其用途

    公开(公告)号：WO0009487A9

    公开(公告)日：2000-06-22

    申请号：PCT/US9918076

    申请日：1999-08-10

    申请人： BAYER AG ,  NATRAJAN ANAND ,  JIANG QINGPING ,  SHARPE DAVID ,  LAW SAY JONG

    发明人： NATRAJAN ANAND ,  JIANG QINGPING ,  SHARPE DAVID ,  LAW SAY-JONG

    IPC分类号： C07D219/04 ,  C07D401/12 ,  C07K14/765 ,  C07K16/26 ,  C09K3/00 ,  C09K11/07 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/76 ,  G01N33/532 ,  G01N33/58 ,  G01N33/533

    CPC分类号： G01N33/582 ,  C07D219/04 ,  Y10S436/80 ,  Y10S436/815 ,  Y10S436/817 ,  Y10S530/812 ,  Y10T436/13

    摘要： Our results thus identify two sets of necessary and sufficient criteria for observing long-wavelength emission from acridinium compounds: Set A: (a) the creation of an extended conjugation system by the attachment of appropriate functional groups on the acridinium nucleus (electronic requirement). (b) Coplanarity of the attached functional group and the acridone moiety during light emission (geometry requirement). (c) Said functional group must consist of at least one aromatic ring and one electron-donating atom or group with an extra pair of electrons which can readily delocalize into the extended pi system to which the heteroatom is directly attached or built into, and establish stable extended resonance with the electron-withdrawing carbonyl moiety of the light emitting acridone. Such electron-donating atom or group that exists in the form of an anion has particularly strong effect to further the bathochromic shift of the emission wavelength. Set B: (a) A direct attachment at one or more of positions C-2, C-4, C-5, or C-7 of the acridinium nucleus, of electron-donating atoms or groups having extra pair(s) of electrons. The electron-donating entities can be the same or different if more than one electron-donating entity is used. Such electron-donating atom or group that exists in the form of an anion has particularly strong effect to further the bathochromic shift of the emission wavelenth. For molecules for which the above criteria are met such as LEAE, 3-HS-DMAE, and 2-hydroxy-DMAE long wavelength-emission exceeding 500 nm and reaching into NIR region is expected and observed. Preferably, the utility of an NIR-AC of comparable quantum yield as the conventional acridinium compounds goes hand-in-hand with the employment of a luminescence detector of good to excellent detection efficiency. To achieve efficient NIR signal detection and facilitate the performing of diagnostic assays, a further objective of the present invention is the advance of a concept and the realization of substituting a state-of-the-art charge-coupled device (CCD) detector for the red-insensitive photomultiplier tube (PMT) in a conventional fully or semi-automatic analyzer such as MLA-II of Chiron Diagnostics, Walpole, MA.

    摘要翻译： 因此，我们的结果确定了观察吖啶鎓化合物长波长发射的两套必要和充分的标准：A组：（a）通过在吖啶鎓核上附加适当的官能团（电子要求）创建扩展的缀合系统。 （b）发光期间附着的官能团和吖啶酮部分的共面性（几何要求）。 （c）所述官能团必须由至少一个芳环和一个供电子原子或基团组成，所述原子或基团具有额外的一对电子，所述电子可以容易地离域到扩展的pI系统中，所述杂原子直接连接或构建到其中， 与发光吖啶酮的吸电子羰基部分稳定的扩展共振。 这种以阴离子形式存在的供电子原子或基团对于进一步发射波长的红移是特别有效的。 组B：（a）在吖啶鎓核的C-2，C-4，C-5或C-7位置的一个或多个位置上直接连接电子给体原子或具有额外的一对或多对 电子。 如果使用多于一个供电子实体，则供电子实体可以相同或不同。 这种以阴离子形式存在的供电子原子或基团对于进一步发射波长的红移是特别有效的。 对于满足上述标准的分子如LEAE，期望并观察到3-HS-DMAE和超过500nm并且到达NIR区域的2-羟基-DMAE长波长发射。 优选地，与传统的吖啶鎓化合物相比，具有可比量子产率的NIR-AC的效用与使用具有良好到极好的检测效率的发光检测器结合在一起。 为了实现有效的NIR信号检测并促进诊断测定的执行，本发明的另一个目的是提出一种概念以及用现有技术的电荷耦合器件（CCD）检测器代替 在传统的全自动或半自动分析仪如马萨诸塞州Walpole的Chiron Diagnostics的MLA-II中使用红色不敏感的光电倍增管（PMT）。