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1.发明申请AN ISOLATED DNA FRAGMENT OF THE HUMAN A33 PROMOTER AND ITS USE TO CONTROL THE EXPRESSION OF A HETEROLOGOUS GENE IN TUMOR CELLS 审中-公开人类A33促进剂的分离的DNA片段及其用于控制肿瘤细胞中异位基因的表达公开(公告)号：WO2007127882A2
公开(公告)日：2007-11-08
申请号：PCT/US2007/067567
申请日：2007-04-26
Applicant: INIS BIOTECH LLC , PODHAJCER, Osvaldo, Luis , CAFFERATA, Eduardo, Gustavo, Alfredo , LOPEZ, Maria, Veronica , MACCIO, Daniela, Rita , VIALE, Diego, Luis
Inventor： PODHAJCER, Osvaldo, Luis , CAFFERATA, Eduardo, Gustavo, Alfredo , LOPEZ, Maria, Veronica , MACCIO, Daniela, Rita , VIALE, Diego, Luis
IPC: A61K48/00
CPC classification number: A61K31/555 , A61K31/513 , A61K31/519 , A61K31/7068 , A61K35/761 , A61K38/00 , A61K38/162 , A61K45/06 , C12N7/00 , C12N15/85 , C12N15/86 , C12N2710/10332 , C12N2710/10343 , C12N2830/002 , C12N2830/85 , A61K2300/00
Abstract: An isolated DNA sequence that corresponds to a region of the human A33 gene promoter from base pair -105 to base pair +307, able to control the expression of a heterologous gene of interest, that may be used in conjunction with any other regulatory sequence, including sequences responsive to stress such as radiation, hypoxia and free radical formation. Constructs and viral vectors for recombinant DNA expression are provided, which include this sequence of human A33 gene promoter and, operably linked thereto, at least one heterologous gene. The regulatory sequence(s) control(s) the expression of at least one heterologous gene in cells including colorectal cancer cells. Pharmaceutical compositions and methods for treating colorectal cancer are also described.
Abstract translation: 分离的DNA序列对应于能够控制感兴趣的异源基因的表达的碱基对-105至碱基对+307的人A33基因启动子的区域，其可以与任何其它调节序列结合使用， 包括响应于诸如辐射，缺氧和自由基形成的应激的序列。 提供用于重组DNA表达的构建体和病毒载体，其包括人类A33基因启动子的该序列，并与其可操作地连接至少一个异源基因。 调节序列控制在包括结肠直肠癌细胞的细胞中至少一种异源基因的表达。 还描述了用于治疗结肠直肠癌的药物组合物和方法。
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2.发明申请AN ISOLATED DNA FRAGMENT OF THE HUMAN A33 PROMOTER AND ITS USE TO CONTROL THE EXPRESSION OF A HETEROLOGOUS GENE IN TUMOR CELLS 审中-公开人类A33促进剂的分离的DNA片段及其用于控制肿瘤细胞中异位基因的表达
公开(公告)号：WO2007127882A3
公开(公告)日：2008-12-04
申请号：PCT/US2007067567
申请日：2007-04-26
Applicant: INIS BIOTECH LLC , PODHAJCER OSVALDO LUIS , CAFFERATA EDUARDO GUSTAVO ALFR , LOPEZ MARIA VERONICA , MACCIO DANIELA RITA , VIALE DIEGO LUIS
Inventor： PODHAJCER OSVALDO LUIS , CAFFERATA EDUARDO GUSTAVO ALFREDO , LOPEZ MARIA VERONICA , MACCIO DANIELA RITA , VIALE DIEGO LUIS
CPC classification number: A61K31/555 , A61K31/513 , A61K31/519 , A61K31/7068 , A61K35/761 , A61K38/00 , A61K38/162 , A61K45/06 , C12N7/00 , C12N15/85 , C12N15/86 , C12N2710/10332 , C12N2710/10343 , C12N2830/002 , C12N2830/85 , A61K2300/00
Abstract: An isolated DNA sequence that corresponds to a region of the human A33 gene promoter from base pair -105 to base pair +307, able to control the expression of a heterologous gene of interest, that may be used in conjunction with any other regulatory sequence, including sequences responsive to stress such as radiation, hypoxia and free radical formation. Constructs and viral vectors for recombinant DNA expression are provided, which include this sequence of human A33 gene promoter and, operably linked thereto, at least one heterologous gene. The regulatory sequence(s) control(s) the expression of at least one heterologous gene in cells including colorectal cancer cells. Pharmaceutical compositions and methods for treating colorectal cancer are also described.
Abstract translation: 分离的DNA序列对应于能够控制感兴趣的异源基因的表达的碱基对-105至碱基对+307的人A33基因启动子的区域，其可以与任何其它调节序列结合使用， 包括响应于诸如辐射，缺氧和自由基形成的应激的序列。 提供用于重组DNA表达的构建体和病毒载体，其包括人类A33基因启动子的该序列，并与其可操作地连接至少一个异源基因。 调节序列控制在包括结肠直肠癌细胞的细胞中至少一种异源基因的表达。 还描述了用于治疗结肠直肠癌的药物组合物和方法。
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公开(公告)号：WO9829138A3
公开(公告)日：1998-09-17
申请号：PCT/GB9703548
申请日：1997-12-24
Applicant: INST DE INVESTIGACIONES BIOQUI , PODHAJCER OSVALDO LUIS , LEDDA MARIA FERNANDA , ADRIS SORAYA KARINA , BRAVO ALICIA INES , MORDOH JOSE , CHERNAJOVSKY YUTI
Inventor： PODHAJCER OSVALDO LUIS , LEDDA MARIA FERNANDA , ADRIS SORAYA KARINA , BRAVO ALICIA INES , MORDOH JOSE , CHERNAJOVSKY YUTI
IPC: A61K38/00 , C12N15/113 , A61K48/00
CPC classification number: C12N15/113 , A61K38/00 , C12N2310/111
Abstract: Compositions and methos are described that decrease or inhibit osteonectin activity in tumour cells, including cancer cells. The cells cease to be tumour-like, or become less tumour-like. Pharmaceutical composition and therapies based thereon are also described. 00000
Abstract translation: 描述了降低或抑制包括癌细胞在内的肿瘤细胞中的骨连接素活性的组合物和methos。 细胞不再是肿瘤状的，或者肿瘤样变小。 还描述了基于其的药物组合物和疗法。 00000
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公开(公告)号：WO1998029138A2
公开(公告)日：1998-07-09
申请号：PCT/GB1997003548
申请日：1997-12-24
Applicant: INSTITUTO DE INVESTIGACIONES BIOQUIMICAS FUNDACION CAMPOMAR , PODHAJCER, Osvaldo Luis , LEDDA, Maria Fernanda , ADRIS, Soraya Karina , BRAVO, Alicia Ines , MORDOH, Jose , CHERNAJOVSKY, Yuti
IPC: A61K48/00
CPC classification number: C12N15/113 , A61K38/00 , C12N2310/111
Abstract: Compositions and methos are described that decrease or inhibit osteonectin activity in tumour cells, including cancer cells. The cells cease to be tumour-like, or become less tumour-like. Pharmaceutical composition and therapies based thereon are also described.
Abstract translation: 描述了降低或抑制包括癌细胞在内的肿瘤细胞中的骨连接素活性的组合物和methos。 细胞不再是肿瘤状的，或者肿瘤样变小。 还描述了基于其的药物组合物和疗法。
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5.发明申请
公开(公告)号：WO2007127347A3
公开(公告)日：2008-01-03
申请号：PCT/US2007010196
申请日：2007-04-26
Applicant: INIS BIOTECH LLC , PODHAJCER OSVALDO LUIS , CAFFERATA EDUARDO GUSTAVO ALFR , LOPEZ MARIA VERONICA , VIALE DIEGO LUIS
Inventor： PODHAJCER OSVALDO LUIS , CAFFERATA EDUARDO GUSTAVO ALFR , LOPEZ MARIA VERONICA , VIALE DIEGO LUIS
CPC classification number: C07K14/4748
Abstract: An isolated DNA sequence corresponding to a region of the SPARC gene human promoter from base pair -513 to base pair +3 5 capable of driving the expression of a heterologous gene of interest, that can be associated to any other promoter sequence, such as radiation responsive sequence, hypoxia responsive sequence and free-radical responsive sequence. The invention also provides constructs and DNA recombinant expression viral vectors, comprising said isolated sequence of the SPARC gene human promoter and at least one heterologous gene operably linked thereto, wherein said promoter sequence drives the expression of the at least one heterologous gene in tumor cells. Pharmaceutical compositions and a method for treating tumors are also provided.
Abstract translation: 对应于能够驱动感兴趣的异源基因表达的从碱基对-513到碱基对+3,5的SPARC基因人启动子的区域的分离的DNA序列，其可以与任何其它启动子序列例如辐射 响应序列，缺氧反应序列和自由基反应序列。 本发明还提供构建体和DNA重组表达病毒载体，其包含SPARC基因人启动子的所述分离序列和与其可操作地连接的至少一个异源基因，其中所述启动子序列驱动肿瘤细胞中至少一种异源基因的表达。 还提供了药物组合物和治疗肿瘤的方法。
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6.发明申请AN ISOLATED DNA FRAGMENT OF THE SPARC HUMAN PROMOTER AND ITS USE FOR DRIVING THE EXPRESSION OF AN HETEROLOGOUS GENE IN TUMOR CELLS 审中-公开SPARC人类促进剂的分离DNA片段及其在肿瘤细胞中驱动异源基因表达的用途
公开(公告)号：WO2007127347A2
公开(公告)日：2007-11-08
申请号：PCT/US2007/010196
申请日：2007-04-26
Applicant: INIS BIOTECH LLC. , PODHAJCER, Osvaldo, Luis , CAFFERATA, Eduardo, Gustavo, Alfredo , LOPEZ, Maria, Veronica , VIALE, Diego, Luis
Inventor： PODHAJCER, Osvaldo, Luis , CAFFERATA, Eduardo, Gustavo, Alfredo , LOPEZ, Maria, Veronica , VIALE, Diego, Luis
IPC: A41D13/05
CPC classification number: C07K14/4748
Abstract: An isolated DNA sequence corresponding to a region of the SPARC gene human promoter from base pair -513 to base pair +3 5 capable of driving the expression of a heterologous gene of interest, that can be associated to any other promoter sequence, such as radiation responsive sequence, hypoxia responsive sequence and free-radical responsive sequence. The invention also provides constructs and DNA recombinant expression viral vectors, comprising said isolated sequence of the SPARC gene human promoter and at least one heterologous gene operably linked thereto, wherein said promoter sequence drives the expression of the at least one heterologous gene in tumor cells. Pharmaceutical compositions and a method for treating tumors are also provided.
Abstract translation: 对应于能够驱动感兴趣的异源基因表达的从碱基对-513到碱基对+3,5的SPARC基因人启动子的区域的分离的DNA序列，其可以与任何其它启动子序列例如辐射 响应序列，缺氧反应序列和自由基反应序列。 本发明还提供构建体和DNA重组表达病毒载体，其包含SPARC基因人启动子的所述分离序列和与其可操作地连接的至少一个异源基因，其中所述启动子序列驱动肿瘤细胞中至少一种异源基因的表达。 还提供了药物组合物和治疗肿瘤的方法。
