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公开(公告)号：WO2016085929A1
公开(公告)日：2016-06-02
申请号：PCT/US2015/062324
申请日：2015-11-24
Applicant: CODEXIS, INC. , THE COCA-COLA COMPANY
Inventor： VROOM, Jonathan , ALVIZO, Oscar , SONI, Pankaj , BROWN, Michael, D. , ENTWISTLE, David
IPC: A23F5/00
CPC classification number: C12P17/182 , C07D473/12 , C12N9/1007 , C12N9/78 , C12Y305/04003
Abstract: The present invention provides an enzymatic means for the biosynthetic production of caffeine. The present invention provides biosynthetic methods for production of caffeine comprising: providing guanine, a guanine deaminase, at least one methyl transferase, and a methyl donor; contacting the guanine with the gtheuanine deaminase to produce xanthine; contacting the xanthine with the methyl transferase and a methyl donor, under conditions wherein the xanthine is methylated, to produce a monomethylxanthine; contacting the monomethylxanthine with the methyl transferase and a methyl donor, under conditions wherein the monomethylxanthine is methylated, to produce a dimethylxanthine; and contacting the dimethylxanthine with the methyl transferase and a methyl donor, under conditions wherein the dimethylxanthine is methylated, to produce caffeine (i.e., 1,3,7- trimethylxanthine).
Abstract translation: 本发明提供了用于生物合成咖啡因的酶法。 本发明提供生产咖啡因的生物合成方法，其包括：提供鸟嘌呤，鸟嘌呤脱氨酶，至少一种甲基转移酶和甲基供体; 使鸟嘌呤与肉毒梭菌脱氨酶接触以产生黄嘌呤; 在黄嘌呤被甲基化的条件下，将黄嘌呤与甲基转移酶和甲基供体接触以产生单甲基黄嘌呤; 在单甲基黄嘌呤被甲基化的条件下，将甲基黄嘌呤与甲基转移酶和甲基供体接触以产生二甲基黄嘌呤; 并在二甲基黄嘌呤被甲基化的条件下使二甲基黄嘌呤与甲基转移酶和甲基供体接触，生成咖啡因（即1,3,7-三甲基黄嘌呤）。
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公开(公告)号：WO2017213758A1
公开(公告)日：2017-12-14
申请号：PCT/US2017/028370
申请日：2017-04-19
Applicant: CODEXIS, INC.
Inventor： NAZOR, Jovana , OSBORNE, Robert , LIANG, Jack , VROOM, Jonathan , ZHANG, Xiyun , ENTWISTLE, David , VOLADRI, Rama , GARCIA, Ravi, David , MOORE, Jeffrey, C. , GROSSER, Shane , KOSJEK, Birgit , TRUPPO, Matthew
Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
Abstract translation: 本发明提供了用于生产羟基化化合物的工程化脯氨酸羟化酶多肽，编码工程化脯氨酸羟化酶的多核苷酸，能够表达工程化脯氨酸羟化酶的宿主细胞，以及使用工程化脯氨酸羟化酶制备 用于生产活性药物的化合物。
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公开(公告)号：WO2018144679A2
公开(公告)日：2018-08-09
申请号：PCT/US2018/016359
申请日：2018-02-01
Applicant: CODEXIS, INC.
Inventor： VROOM, Jonathan , GALANIE, Stephanie, Sue , DELLAS, Nikki , LIANG, Jack , LIU, Joyce , ENTWISTLE, David , MOFFETT, Courtney, Dianne
Abstract: The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.
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公开(公告)号：WO2021207208A
公开(公告)日：2021-10-14
申请号：PCT/US2021/025975
申请日：2021-04-06
Applicant: CODEXIS, INC. [US]/[US]
Inventor： VOLADRI, Rama , HURTAK, Jessica Anna , SOWELL-KANTZ, Auric Anthony , SUBRAMANIAN, Nandhitha , BERMUDEZ, Ericka , ENTWISTLE, David , MAYO, Melissa Ann , RIGGINS, James Nicholas , NOVICK, Scott J.
IPC: C12N9/18
Abstract: The present invention provides engineered carboxyesterase enzymes having improved properties as compared to a naturally occurring wild-type carboxyesterase enzymes, as well as polynucleotides encoding the engineered carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes in hydrolysis reactions.
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公开(公告)号：WO2020013951A1
公开(公告)日：2020-01-16
申请号：PCT/US2019/037205
申请日：2019-06-14
Applicant: CODEXIS, INC.
Inventor： NOVICK, Scott, J. , GARCIA, Ravi, David , CHING, Charlene , DELLAS, Nikki , ENTWISTLE, David , MITCHELL, Vesna
Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.
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公开(公告)号：WO2018231462A1
公开(公告)日：2018-12-20
申请号：PCT/US2018/034069
申请日：2018-05-23
Applicant: CODEXIS, INC.
Inventor： NOVICK, Scott, J. , DELLAS, Nikki , ALVIZO, Oscar , GARCIA, Ravi, David , CHING, Charlene , ENTWISTLE, David
Abstract: The present invention provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The invention also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
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公开(公告)号：WO2022066534A1
公开(公告)日：2022-03-31
申请号：PCT/US2021/050944
申请日：2021-09-17
Applicant: CODEXIS, INC.
Inventor： SIVARAMAKRISHNAN, Santhosh , BERMUDEZ, Ericka , SUBRAMANIAN, Nandhitha , ENTWISTLE, David , FORGET, Stephanie, Marie
Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
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公开(公告)号：WO2019118557A1
公开(公告)日：2019-06-20
申请号：PCT/US2018/065124
申请日：2018-12-12
Applicant: CODEXIS, INC.
Inventor： VOLADRI, Rama , ENTWISTLE, David , MICKLITSCH, Christopher, Michael , xMITCHELL, Vesna , DELLAS, Nikki , ZHANG, Xiyun , SERO, Antoinette , DORR, Brent , FUERST, Douglas, E.
IPC: C12N9/18 , A23L3/3571
Abstract: The present invention provides engineered carboxyesterase enzymes having improved properties as compared to a naturally occurring wild-type carboxyesterase enzymes, as well as polynucleotides encoding the engineered carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes in amidation reactions.
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公开(公告)号：WO2018144675A1
公开(公告)日：2018-08-09
申请号：PCT/US2018/016352
申请日：2018-02-01
Applicant: CODEXIS, INC.
Inventor： VROOM, Jonathan , GALANIE, Stephanie, Sue , DELLAS, Nikki , LIANG, Jack , LIU, Joyce , ENTWISTLE, David , MOFFETT, Courtney, Dianne
Abstract: The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.
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公开(公告)号：WO2020028039A1
公开(公告)日：2020-02-06
申请号：PCT/US2019/042151
申请日：2019-07-17
Applicant: CODEXIS, INC. , TATE & LYLE INGREDIENTS AMERICAS LLC
Inventor： VROOM, Jonathan , GALANIE, Stephanie, Sue , LIANG, Jack , LIU, Joyce , DELLAS, Nikki , MAYO, Melissa, Ann , ENTWISTLE, David
Abstract: The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.
