公开(公告)号：WO2014140167A1

公开(公告)日：2014-09-18

申请号：PCT/EP2014/054949

申请日：2014-03-13

Applicant: DSM IP ASSETS B.V. ,  CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12P19/02 ,  C12P19/14 ,  C12N9/24 ,  C12P7/10

CPC classification number: C12P7/10 ,  C12N9/2437 ,  C12N9/2442 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C12Y302/01 ,  C12Y302/01014 ,  Y02E50/16

Abstract: The present invention relates to a novel process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising: a. a polypeptide comprising an amino acid sequence of any one of SEQ ID NOs: 5 or 6; b. a polypeptide comprising an amino acid sequence that is at least 70%, preferably at least 80%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% amino acid sequence identity to the polypeptide defined in (a); c. a polypeptide comprising an amino acid sequence encoded by the nucleic acid sequence of any one of SEQ ID NOs: 1-4; d. a polypeptide comprising an amino acid sequence encoded by a polynucleotide molecule that hybridizes under medium-high stringency conditions, high stringency conditions, or very high stringency conditions with the full-length complement of a polynucleotide molecule comprising the nucleic acid sequence of any one of SEQ ID NOs: 1-4; e. a polypeptide comprising an amino acid sequence encoded by a polynucleotide molecule having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% nucleic acid sequence identity to a polynucleotide comprising the nucleic acid sequence of any one of SEQ ID NOs: 1-4; or f. a polypeptide comprising an amino acid sequence encoded by any one of the exonic nucleic acid sequences corresponding to positions: 1-194, 276-568, 639-809, 869-1143, 1235-1322, 1386-1797, 1872-2040of SEQ ID NO: 1; 1-729 of SEQ ID NO: 2.

Abstract translation: 本发明涉及将生物质或预处理的生物质降解成糖的新方法，其中使用的酶包括：a。 包含SEQ ID NO：5或6中任一个的氨基酸序列的多肽; 湾 包含至少70％，优选至少80％，更优选至少90％，甚至更优选至少95％，96％，97％，98％或99％氨基酸序列同一性的氨基酸序列的多肽 （a）中定义的多肽; C。 包含由SEQ ID NO：1-4中任一个的核酸序列编码的氨基酸序列的多肽; 天。 包含由多核苷酸分子编码的氨基酸序列的多肽，其在中高度严格条件，高严格条件或非常高严格条件下与包含SEQ ID NO中任一个核酸序列的多核苷酸分子的全长互补序列杂交 身份证号：1-4; 即 包含由多核苷酸分子编码的氨基酸序列的多肽，所述多核苷酸分子至少具有70％，至少75％，至少80％，至少85％，至少90％，至少91％，至少92％ 与包含SEQ ID NO中任一个核酸序列的多核苷酸的核酸序列同一性为93％，至少94％，至少95％，至少96％，至少97％，至少98％，或至少99％ 身份证号：1-4; 或f。 包含由对应于SEQ ID NO：1-194,276-568,639-809,869-1143,1235-1322,1386-1797,1872-2040的任一个外显子核酸序列编码的氨基酸序列的多肽 NO：1; 1-729为SEQ ID NO：2。

公开(公告)号：WO2014140171A1

公开(公告)日：2014-09-18

申请号：PCT/EP2014/054954

申请日：2014-03-13

Applicant: DSM IP ASSETS B.V. ,  CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  BUTLER, Gregory ,  POWLOWSKI, Justin

IPC: C12P19/02 ,  C12P19/14 ,  C13K1/02 ,  C12N9/42 ,  C12P7/10

CPC classification number: C12P19/02 ,  C12P7/10 ,  C12P19/14 ,  C12Y302/01091 ,  C13K1/02 ,  Y02E50/16

Abstract: The present invention relates to a novel process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a. a polypeptide comprising an amino acid sequence of any one of SEQ ID NOs: 7- 9; b. a polypeptide comprising an amino acid sequence that is at least 85%, preferably at least 90%, more preferably at least 95%, even more preferably at least 96%, 97%, 98% or 99% amino acid sequence identity to the polypeptide defined in (a); c. a polypeptide comprising an amino acid sequence encoded by the nucleic acid sequence of any one of SEQ ID NOs: 1 -6; d. a polypeptide comprising an amino acid sequence encoded by a polynucleotide molecule that hybridizes under medium-high stringency conditions, high stringency conditions, or very high stringency conditions with the full-length complement of a polynucleotide molecule comprising the nucleic acid sequence of any one of SEQ ID NOs: 1 -6; e. a polypeptide comprising an amino acid sequence encoded by a polynucleotide molecule having at least 85%, at least 90%, at least 91 %, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% nucleic acid sequence identity to a polynucleotide comprising the nucleic acid sequence of any one of SEQ ID NOs: 1 -6; or f. a polypeptide comprising an amino acid sequence encoded by any one of the exonic nucleic acid sequences corresponding to positions: 1-92, 150-654, 815-1385, 1452-1714 of SEQ ID NO: 2; 1 -246, 324-1007, 1082-1 135 of SEQ ID NO: 1; 1-394, 506-789 of SEQ ID NO: 3.

Abstract translation: 本发明涉及将生物质或预处理的生物质降解成糖的新方法，其中使用的酶包括a。 包含SEQ ID NO：7-9中任一个的氨基酸序列的多肽; 湾 一种多肽，其包含与多肽的至少85％，优选至少90％，更优选至少95％，甚至更优选至少96％，97％，98％或99％氨基酸序列同一性的氨基酸序列 （a）中定义; C。 包含由SEQ ID NO：1-6中任一个的核酸序列编码的氨基酸序列的多肽; 天。 包含由多核苷酸分子编码的氨基酸序列的多肽，其在中高度严格条件，高严格条件或非常高严格条件下与包含SEQ ID NO中任一个核酸序列的多核苷酸分子的全长互补序列杂交 身份证号：1 -6; 即 包含由多核苷酸分子编码的氨基酸序列的多肽，所述多核苷酸分子至少具有85％，至少90％，至少91％，至少92％，至少93％，至少94％，至少95％ 与包含SEQ ID NO：1-6中任一个的核酸序列的多核苷酸的核酸序列同一性为96％，至少97％，至少98％或至少99％ 或f。 包含由对应于SEQ ID NO：2的1-92,150-654,815-1385,1452-1714位置的任何一个外显子核酸序列编码的氨基酸序列的多肽; SEQ ID NO：1的1 -246,324-1007,1028-135; SEQ ID NO：3的1-394,506-789。

公开(公告)号：WO2014140165A1

公开(公告)日：2014-09-18

申请号：PCT/EP2014/054946

申请日：2014-03-13

Applicant: DSM IP ASSETS B.V. ,  CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12P19/02 ,  C12P19/14 ,  C12N9/24 ,  C12P7/10

CPC classification number: C12P19/02 ,  C12N9/2402 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C12Y302/01021 ,  Y02E50/16

Abstract: The present invention relates to a process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a.a polypeptide comprising an amino acid sequence of any one of SEQ ID NOs: or 6; b.a polypeptide comprising an amino acid sequence that is at least 85%, preferably at least 90%, more preferably at least 95%, even more preferably at least 96%, 97%, 98% or 99%amino acid sequence identity to the polypeptide defined in (a); c.a polypeptide comprising an amino acid sequence encoded by the nucleic acid sequence of any one of SEQ ID NOs: 1-4; d.a polypeptide comprising an amino acid sequence encoded by a polynucleotide molecule that hybridizes under medium-high stringency conditions, high stringency conditions, or very high stringency conditions with the full-length complement of a polynucleotide molecule comprising the nucleic acid sequence of any one of SEQ ID NOs: 1-4; e.a polypeptide comprising an amino acid sequence encoded by a polynucleotide molecule having at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% nucleic acid sequence identity to a polynucleotide comprising the nucleic acid sequence of any one of SEQ ID NOs: 1-4; or f.a polypeptide comprising an amino acid sequence encoded by any one of the exonic nucleic acid sequences corresponding to positions: 1-60, 139-278, 341-385, 445- 497, 556-2834 of SEQ ID NO: 1; 1-101, 154-823 SEQ ID NO: 2.

Abstract translation: 本发明涉及将生物质或预处理的生物质降解成糖的方法，其中使用包含a.a多肽的酶，所述多肽包含SEQ ID NO：或6中任一个的氨基酸序列; ba多肽，其包含与多肽的至少85％，优选至少90％，更优选至少95％，甚至更优选至少96％，97％，98％或99％氨基酸序列同一性的氨基酸序列 （a）中定义; c.a多肽，其包含由SEQ ID NO：1-4中任一个的核酸序列编码的氨基酸序列; da多肽，其包含由多核苷酸分子编码的氨基酸序列，其在中高度严格条件，高严格条件或非常高严格条件下与包含SEQ ID NO中任一个核酸序列的多核苷酸分子的全长互补序列杂交 身份证号：1-4; ea多肽，其包含由多核苷酸分子编码的氨基酸序列，所述多核苷酸分子至少具有85％，至少90％，至少91％，至少92％，至少93％，至少94％，至少95％ 与包含SEQ ID NO：1-4中任一个的核酸序列的多核苷酸的核酸序列同一性为96％，至少97％，至少98％或至少99％ 或f.a多肽，其包含由对应于SEQ ID NO：1的1-60,139-278,341-385,445-497,556-2834的任一个的外显子核酸序列编码的氨基酸序列; 1-101,154-823 SEQ ID NO：2。

公开(公告)号：WO2014060380A1

公开(公告)日：2014-04-24

申请号：PCT/EP2013/071466

申请日：2013-10-15

Applicant: DSM IP ASSETS B.V. ,  CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12P19/14 ,  C12P7/10

CPC classification number: C12P7/10 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/16

Abstract: The present invention relates tonovel Thermoascus aurantiacus enzymes or proteins for cell wall deconstruction, polynucleotide sequences encoding the polypeptides according to the invention, a production process for the enzymes according to the invention and the use of the enzymes according to the invention in various industrial processes.

Abstract translation: 本发明涉及用于细胞壁解构的tonovel辣根过氧化物酶或蛋白质，编码根据本发明的多肽的多核苷酸序列，根据本发明的酶的制备方法和根据本发明的酶在各种工业过程中的用途。

公开(公告)号：WO2014059541A1

公开(公告)日：2014-04-24

申请号：PCT/CA2013/050778

申请日：2013-10-15

Applicant: CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12N15/55 ,  A21D13/06 ,  A23K1/16 ,  A23L1/28 ,  A23L1/305 ,  C07K14/37 ,  C07K16/14 ,  C07K16/40 ,  C12N15/31 ,  C12N15/52 ,  C12N15/53 ,  C12N15/63 ,  C12N15/80 ,  C12N9/00 ,  C12N9/02 ,  C12N9/14 ,  C12P7/06 ,  D06M16/00 ,  D06P5/00 ,  D21C5/00 ,  D21C9/00

CPC classification number: D06M16/003 ,  A21D8/042 ,  A23K10/14 ,  A23K10/30 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  A23L29/06 ,  C07K14/37 ,  C12N9/00 ,  C12N9/14 ,  C12N9/16 ,  C12N9/24 ,  C12N15/52 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21C11/0007 ,  D21H17/005 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi, Thermoascus aurantiacus strain CBS 181.67, Myceliophthora fergusii (Corynascus thermophilus) strain CBS 405.69, and Pseudocercosporella herpotrichoides strain 494.80. Uses thereof in various industrial processes such as in biofuels, food preparation, animal feed, pulp and paper, textiles, detergents, waste treatment and others are also disclosed.

Abstract translation: 本发明涉及具有与生物量加工和/或降解（例如，细胞壁解构）有关的活性的新型多肽和酶（例如热稳定蛋白和酶），以及与其相关的多核苷酸，载体，细胞，组合物和工具 ，或其功能变体。 更具体地说，本发明涉及可从真菌分离的分泌的酶，例如嗜热黄曲霉菌株CBS 181.67，嗜热丝孢杆菌（Corynascus thermophilus）菌株CBS 405.69和假铁孢杆菌菌株494.80。 还公开了在生物燃料，食品制备，动物饲料，纸浆和纸，纺织品，洗涤剂，废物处理等各种工业过程中的应用。

公开(公告)号：WO2013182671A1

公开(公告)日：2013-12-12

申请号：PCT/EP2013/061769

申请日：2013-06-07

Applicant: DSM IP ASSETS B.V. ,  CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C07K14/37 ,  C12N9/42

CPC classification number: C12P7/10 ,  C12N9/2434 ,  C12N9/2437 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/16

Abstract: The present invention relates to process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a.a polypeptide sequence as set forth in any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 44, 47 and 50; b.a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%,98% or 99% homologous to the any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 44, 47 and 50; c.a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 7, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 45, 46, 48 and 50, or nucleic acids that are at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 9, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 45, 46, 48 and 50; d.a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 7, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 45, 46, 48 and 50; or e.a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 45, 46, 48 and 50.

Abstract translation: 本发明涉及将生物质或预处理的生物质降解成糖的方法，其中使用的酶包括具有如SEQ ID No：3,6,9,12,15,18,21中任一项所示的多肽序列的多肽 ，24,27,30,33,36,39,42,44,47和50; ba多肽，其与任何一种的至少60％，优选至少70％，更优选至少80％，甚至更优选至少90％，95％，96％，97％，98％或99％同源 SEQ ID No：3,6,9,12,15,18,21,24,27,30,33,36,39,42,44,47和50; 由SEQ ID NO：1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23中任一个所示的核酸序列编码的多肽序列 ，25,26,28,29,31,32,34,35,37,38,40,41,43,45,46,48和50，或至少60％，优选至少70％的核酸， ，更优选至少80％，甚至更优选至少90％，95％，96％，97％，98％或99％同源于SEQ ID NO：1,2,4,5,7,8 ，10，11，13，14，16，17，9，20，22，23，25，26，28，29，31，32，34，35，37，38，40，41，43，45，46 ，48和50; da多肽序列，其由在严格条件下与如SEQ ID NO：2,4,5,7,8,10,11,13,14,16,7中任一个所述的多核苷酸杂交的核酸序列编码 ，19，20，22，23，25，26，28，29，31，32，34，35，37，38，40，41，43，45，46，48及50条; 或由在严格条件下杂交的核酸序列编码的ea多肽序列与如SEQ ID NO：1,2,4,5,7,8,10,11,13， 14，16，17，19，20，22，23，25，26，28，29，31，32，34，35，37，38，40，41，43，45，46，48及50条。

公开(公告)号：WO2013182670A2

公开(公告)日：2013-12-12

申请号：PCT/EP2013/061768

申请日：2013-06-07

Applicant: DSM IP ASSETS B.V. ,  CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12N9/88

CPC classification number: C12N9/24 ,  C07K14/37 ,  C12N9/18 ,  C12N9/2402

Abstract: The present invention relates to process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a. a polypeptide sequence as set forth in any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 62 and 64; b. a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 62 and 64; c. a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 7, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61 and 63, or nucleic acids that are at least 60%, preferably at least 70%, more 2 preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 8, 59, 61 and 63;30 d. a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set DSM IP Assets B.V. 28984-WO-PCT forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 7, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61 and 63; or e. a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50,52, 53, 55, 56, 58, 59, 61 and 63.

Abstract translation: 本发明涉及将生物质或预处理的生物质降解成糖的方法，其中使用的酶包括具有α的多肽。 3，6，9，12，15，18，21，24，27，30，33，36，39，42，45，48，51，54， 57，60，62和64; 湾 与下列任何一种的至少60％，优选至少70％，更优选至少80％，甚至更优选至少90％，95％，96％，97％，98％或99％同源的多肽 SEQ ID No：3,6,9,12,15,18,21,24,27,30,33,36,39,42,45,48,51,54,57,60,62和64; C。 由SEQ ID NO：1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23中任一个所示的核酸序列编码的多肽序列 ，25,26,28,29,31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53,55,56,58,59,61 和63，或者至少60％，优选至少70％，更优选至少80％，甚至更优选至少90％，95％，96％，97％，98％或99％同源的核酸 具有SEQ ID NO：1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23,25,26,28,29,31,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39,39 32，34，35，37，38，40，41，43，44，46，47，49，50，52，53，55，56，8，59，61和63; 30 d。 由在严格条件下与多核苷酸杂交的核酸序列编码的多肽序列如SEQ ID NO：1,2,4,5,7,8,10， 11，13，14，16，7，19，20，22，23，25，26，28，29，31，32，34，35，37，38，40，41，43，44，46，47， 49，50，52，53，55，56，58，59，61和63; 或e。 由在严格条件下与SEQ ID NO：2,4,5,7,8,10,11,13,14中任一项所示的多核苷酸的反向互补序列杂交的核酸序列编码的多肽序列 ，16，17，19，20，22，23，25，26，28，29，31，32，34，35，37，38，40，41，43，44，46，47，49，50,52 ，53,55,56,58,59,61和63。

公开(公告)号：WO2016090472A1

公开(公告)日：2016-06-16

申请号：PCT/CA2015/051283

申请日：2015-12-08

Applicant: CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12N15/52 ,  A21D8/04 ,  A23K20/189 ,  A23L33/17 ,  C07K14/37 ,  C12N1/15 ,  C12N1/21 ,  C12N15/31 ,  C12N15/53 ,  C12N15/55 ,  C12N15/56 ,  C12N15/57 ,  C12N15/60 ,  C12N15/63 ,  C12N15/80 ,  C12N9/00 ,  C12N9/02 ,  C12N9/14 ,  C12N9/16 ,  C12N9/24 ,  C12N9/48 ,  C12N9/88 ,  C12P19/00 ,  C12P21/02 ,  C12P7/06 ,  D06M16/00 ,  D21C1/00

CPC classification number: C12N9/00 ,  A21D8/042 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  C07K14/37 ,  C12N9/24 ,  C12N9/48 ,  C12N15/52 ,  C12P7/08 ,  C12P19/14 ,  D21C5/005 ,  D21H17/005 ,  Y02E50/17

Abstract: The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi Remersonia thermophila (Stilbella thermophila) strain ATCC 22073, Melanocarpus albomyces strain ATCC 6460, and Lentinula edodes strain ATCC 48564. Uses thereof in various industrial processes such as in biofuels, food preparation, animal feed, pulp and paper, textiles, detergents, waste treatment and others are also disclosed.

Abstract translation: 本发明涉及具有与生物量加工和/或降解（例如，细胞壁解构）有关的活性的新型多肽和酶（例如热稳定蛋白和酶），以及与其相关的多核苷酸，载体，细胞，组合物和工具 ，或其功能变体。 更具体地说，本发明涉及可从真菌Remersonia thermophila（Stilbella thermophila）菌株ATCC 22073，Melanocarpus albomyces菌株ATCC 6460和Lentinula edodes菌株ATCC 48564分离的分泌的酶。用于各种工业过程如生物燃料 ，食品制备，动物饲料，纸浆和纸张，纺织品，洗涤剂，废物处理等。

公开(公告)号：WO2015109405A1

公开(公告)日：2015-07-30

申请号：PCT/CA2015/050042

申请日：2015-01-22

Applicant: CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12N15/56 ,  A21D2/26 ,  A23K1/165 ,  A23L1/305 ,  C07K14/37 ,  C12N1/15 ,  C12N1/21 ,  C12N15/31 ,  C12N15/52 ,  C12N15/55 ,  C12N15/57 ,  C12N15/63 ,  C12N15/80 ,  C12N9/00 ,  C12N9/02 ,  C12N9/14 ,  C12N9/24 ,  C12N9/42 ,  C12N9/58 ,  C12N9/88 ,  C12P19/00 ,  C12P19/14 ,  C12P21/02 ,  C12P7/10 ,  D06M16/00 ,  D21C9/00

CPC classification number: C12P19/00 ,  A21D8/042 ,  A23K20/189 ,  C12N9/00 ,  C12N9/14 ,  C12N9/24 ,  C12N9/2437 ,  C12N9/2482 ,  C12N9/58 ,  C12N9/88 ,  C12P7/10 ,  C12P19/14 ,  D06M16/003 ,  D06P1/228 ,  D06P5/13 ,  D06P5/15 ,  D21C5/005 ,  Y02E50/16

Abstract: The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi Chaetomium thermophilum strain ATCC 16451, Thermomyces stellatus strain CBS 241.64, and Corynascus sepedonium strain ATCC 9787. Uses thereof in various industrial processes such as in biofuels, food preparation, animal feed, pulp and paper, textiles, detergents, waste treatment and others are also disclosed.

Abstract translation: 本发明涉及具有与生物量加工和/或降解（例如，细胞壁解构）有关的活性的新型多肽和酶（例如热稳定蛋白和酶），以及与其相关的多核苷酸，载体，细胞，组合物和工具 ，或其功能变体。 更具体地说，本发明涉及可从真菌毛壳菌嗜热菌菌株ATCC16451，嗜热丝菌菌菌株CBS 241.64和克氏锥虫菌株ATCC9787分离的分泌的酶。在各种工业过程中使用它们，例如在生物燃料，食品制备， 还公开了动物饲料，纸浆和纸，纺织品，洗涤剂，废物处理等。

公开(公告)号：WO2014110675A1

公开(公告)日：2014-07-24

申请号：PCT/CA2014/050024

申请日：2014-01-16

Applicant: CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12N15/57 ,  A21D2/26 ,  A23K1/165 ,  A23L1/305 ,  C07K14/37 ,  C07K16/14 ,  C07K16/40 ,  C12N15/31 ,  C12N15/55 ,  C12N15/56 ,  C12N15/63 ,  C12N15/80 ,  C12N9/14 ,  C12N9/16 ,  C12N9/24 ,  C12N9/58 ,  C12P7/06 ,  D06M16/00 ,  D06P5/00 ,  D21B1/02 ,  D21C5/00 ,  D21C9/00

CPC classification number: D06M16/003 ,  A21D8/042 ,  A23K10/30 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  A23L29/06 ,  C12N9/0055 ,  C12N9/0065 ,  C12N9/14 ,  C12N9/88 ,  D06L4/40 ,  D06P5/158 ,  D21C5/005 ,  D21H17/005 ,  Y02E50/17

Abstract: The present invention relates to novel polypeptides and enzymes (e.g., thermostable proteins and enzymes) having activities relating to biomass processing and/or degradation (e.g., cell wall deconstruction), as well as polynucleotides, vectors, cells, compositions and tools relating to same, or functional variants thereof. More particularly, the present invention relates to secreted enzymes that may be isolated from the fungi, Amorphotheca resinae strain DAOM194228, Rhizomucor pusillus strain CBS 183.67, and Calcarisporiella thermophila strain CBS 279.70. Uses thereof in various industrial processes such as in biofuels, food preparation, animal feed, pulp and paper, textiles, detergents, waste treatment and others are also disclosed.

Abstract translation: 本发明涉及具有与生物量加工和/或降解（例如，细胞壁解构）有关的活性的新型多肽和酶（例如热稳定蛋白和酶），以及与其相关的多核苷酸，载体，细胞，组合物和工具 ，或其功能变体。 更具体地说，本发明涉及可从真菌中分离的分泌的酶，天竺葵属植物菌株DAOM194228，根毛霉菌株CBS 183.67和嗜热镰刀菌菌株CBS 279.70。 还公开了在生物燃料，食品制备，动物饲料，纸浆和纸，纺织品，洗涤剂，废物处理等各种工业过程中的应用。