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公开(公告)号：WO2016061438A1
公开(公告)日：2016-04-21
申请号：PCT/US2015/055900
申请日：2015-10-16
Applicant: DANISCO US INC.
Inventor： KOLKMAN, Marc , GOEDEGEBUUR, Frits , MULDER, Harm , BOTT, Richard R. , KELLETT-SMITH, Anja Hemmingsen , BABE, Lilia Maria
IPC: C12N9/54
CPC classification number: C12N9/54 , C11D3/001 , C11D3/386 , C11D11/0017 , C11D11/0023 , C12Y304/21062
Abstract: The present disclosure relates to serine proteases cloned from Bacillus spp ., and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
Abstract translation: 本公开涉及从芽孢杆菌属克隆的丝氨酸蛋白酶及其变体。 含有丝氨酸蛋白酶的组合物适用于清洁织物和硬表面，以及各种工业应用。
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公开(公告)号：WO2009048487A1
公开(公告)日：2009-04-16
申请号：PCT/US2008/004556
申请日：2008-04-08
Applicant: DANISCO US, INC., GENENCOR DIVISION , AEHLE, Wolfgang , BOTT, Richard R. , NIKOLAEV, Igor , SCHEFFERS, Martijn , VAN SOLINGEN, Piet , VROEMEN, Casper
Inventor： AEHLE, Wolfgang , BOTT, Richard R. , NIKOLAEV, Igor , SCHEFFERS, Martijn , VAN SOLINGEN, Piet , VROEMEN, Casper
CPC classification number: C12N9/2428 , Y02E50/17
Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
Abstract translation: 本发明涉及葡糖淀粉酶变体。 特别地，本发明涉及葡糖淀粉酶的淀粉结合结构域（SBD）中的变体。 本发明还涉及与相应的母体葡糖淀粉酶相比具有改变的性质（例如，改善的热稳定性和/或增加的比活性）的变体。 本发明还提供了包含变体葡糖淀粉酶的酶组合物; 包含编码变体的多核苷酸的DNA构建体; 以及在宿主细胞中产生葡糖淀粉酶变体的方法。
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公开(公告)号：WO2017106676A1
公开(公告)日：2017-06-22
申请号：PCT/US2016/067223
申请日：2016-12-16
Applicant: DANISCO US INC
Inventor： PANDEY, Neeraj , BOTT, Richard R. , PRICELIUS, Sina , SACHAN, Annapurna , SHIPOVSKOV, Stepan , SATAPATHY, Ajit
CPC classification number: C12N9/24 , C12N9/2437 , C12Y302/01004
Abstract: Disclosed herein are cellulase variants, or active fragments thereof, and polynucleotides encoding same, wherein the cellulase variants, or active fragments thereof, hav endoglucanase activity. Also disclosed herein are compositions comprising said cellulase variants, or active fragments thereof; vectors and/or host cells comprising the polynucleotides encoding said cellulase variants, or active fragments thereof; and methods for making and/or using said cellulase variants, or active fragments thereof and/or compositions containing same; wherein said cellulase variants, or active fragments thereof, have endoglucanase activity.
Abstract translation: 本文公开了纤维素酶变体或其活性片段和编码它们的多核苷酸，其中纤维素酶变体或其活性片段具有内切葡聚糖酶活性。 本文还公开了包含所述纤维素酶变体或其活性片段的组合物; 包含编码所述纤维素酶变体的多核苷酸或其活性片段的载体和/或宿主细胞; 以及制造和/或使用所述纤维素酶变体或其活性片段和/或含有它们的组合物的方法; 其中所述纤维素酶变体或其活性片段具有内切葡聚糖酶活性。
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公开(公告)号：WO2015089447A1
公开(公告)日：2015-06-18
申请号：PCT/US2014/070107
申请日：2014-12-12
Applicant: DANISCO US INC.
Inventor： KOLKMAN, Marc , MEJLDAL, Rie , GOEDEGEBUUR, Frits , BABE, Lilia Maria , KELLETT-SMITH, Anja Hemmingsen , MULDER, Harm , BOTT, Richard R. , SCOTCHER, Miles Christopher
IPC: C12N9/54
CPC classification number: C12N9/54 , A47L15/0002 , A47L2601/20 , C11D3/386 , C12Y304/21
Abstract: The present disclosure relates to serine proteases cloned from Bacillus gibsonii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
Abstract translation: 本公开涉及从芽孢杆菌（Gibsonii）克隆的丝氨酸蛋白酶及其变体。 含有丝氨酸蛋白酶的组合物适用于清洁织物和硬表面，以及各种工业应用。
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公开(公告)号：WO2014093287A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/074020
申请日：2013-12-10
Applicant: DANISCO US INC.
Inventor： BOTT, Richard R. , FOUKARAKI, Maria , HOMMES, Ronaldus , KAPER, Thijs , KELEMEN, Bradley R. , KRALJ, Slavko , NIKOLAEV, Igor , SANDGREN, Mats , VAN LIESHOUT, Johannes , VAN STIGT THANS, Sander
CPC classification number: C12N9/2437 , C11D3/38645 , C11D3/38681 , C12P19/02 , C12P19/14 , C12Y302/01091 , C12Y302/01099
Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
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公开(公告)号：WO2014093281A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/074011
申请日：2013-12-10
Applicant: DANISCO US INC.
Inventor： BOTT, Richard R. , FOUKARAKI, Maria , HOMMES, Ronaldus , KAPER, Thijs , KELEMEN, Bradley R. , KRALJ, Slavko , NIKOLAEV, Igor , SANDGREN, Mats , VAN LIESHOUT, Johannes , VAN STIGT THANS, Sander
CPC classification number: C12N9/2437 , C12N9/2434 , C12P19/02 , C12P19/14 , C12Y302/01004
Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
Abstract translation: 公开了许多Hypocrea jecorina Cel7A（以前的里氏木霉纤维二糖水解酶I或CBH1）的同源物和变体，编码它们的核酸及其制备方法。 同系物和变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代和/或缺失。
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7.发明申请COMPOSITIONS AND METHODS COMPRISING LG12-CLADE PROTEASE VARIANTS 审中-公开包含LG12-CLADE PROTEASE变体的组合物和方法
公开(公告)号：WO2016145428A1
公开(公告)日：2016-09-15
申请号：PCT/US2016/022282
申请日：2016-03-14
Applicant: DANISCO US INC , MULDER, Harm , MILLER, Jeffrey A. , GOEDEGEBUUR, Frits , ESTELL, David A.
Inventor： MULDER, Harm , MILLER, Jeffrey A. , GOEDEGEBUUR, Frits , ESTELL, David A. , AUGUSTYN, Katherine , BABE, Lilia Maria , BOTT, Richard R. , SCOTCHER, Miles Christopher , YAO, Jian
IPC: C12N9/54
CPC classification number: C12N9/54 , C11D3/386 , C11D3/38663 , C11D3/38681 , C12Y304/21062
Abstract: The present disclosure provides LG12-clade enzyme variants, compositions comprising these enzyme variants, and method of using these enzymes and compositions, as well as the polynucleotides encoding these enzymes, the vectors comprising these polynucleotides, and the host cells transformed with such vectors.
Abstract translation: 本公开提供了LG12-分化酶变体，包含这些酶变体的组合物，以及使用这些酶和组合物的方法，以及编码这些酶的多核苷酸，包含这些多核苷酸的载体以及用这些载体转化的宿主细胞。
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8.发明申请ALPHA-AMYLASES FROM A SUBSET OF EXIGUOBACTERIUM, AND METHODS OF USE, THEREOF 审中-公开来自一个子宫的ALPHA-淀粉及其使用方法
公开(公告)号：WO2015050724A1
公开(公告)日：2015-04-09
申请号：PCT/US2014/056650
申请日：2014-09-19
Applicant: DANISCO US INC.
Inventor： LIU, Guoqing , HUA, Ling , QIAN, Zhen , SONG, Danfeng , TANG, Zhongmei , XIE, Zhiyong , ZHANG, Bo , ZOU, Zhengzheng , XU, Wei , BOTT, Richard R.
IPC: C12N9/28
CPC classification number: C11D3/386 , C12N9/2417 , C12Y302/01001
Abstract: Disclosed are compositions and methods relating to alpha-amylase from a subset of Exiguobacterium. The compositions and methods are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Abstract translation: 公开了与来自Exiguobacterium的子集的α-淀粉酶有关的组合物和方法。 组合物和方法可用于例如淀粉液化和糖化，用于清洁衣物，餐具洗涤和其它应用中的淀粉污渍，用于织物加工（例如脱浆），用于改善消化率的动物饲料中，以及烘烤和酿造 。
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公开(公告)号：WO2014007921A1
公开(公告)日：2014-01-09
申请号：PCT/US2013/042702
申请日：2013-05-24
Applicant: DANISCO US INC.
Inventor： BOTT, Richard R. , CASCAO-PEREIRA, Luis G. , ESTELL, David A. , KOLKMAN, Marc , WILDES, David E.
IPC: C12N9/26
CPC classification number: C12N9/2414 , A23L2/382 , A23V2002/00 , C11D3/386 , C11D11/0017 , C11D11/0023 , C12N9/2417 , C12N9/242 , C12P19/02 , C12P19/14 , C12Y302/01001
Abstract: Described are variants of alpha-amylase enzymes for use in industrial processes, such as liquefaction of starch. The alpha-amylase variants have increased specific activity allowing the more rapidly reduction of peak viscosity during liquefaction processes. The alpha-amylase is modified by introducing into the amino sequence of a parent Family 13 alpha-amylase polypeptide a mutation at an amino acid residue in the starch-binding groove; wherein the starch-binding groove is formed by amino acid residues in the alpha-helix preceding the first beta-strand in the A domain, the loop between the sixth alpha-helix and the seventh beta-strand in the A domain, the loop between the seventh alpha-helix and the eighth beta-strand in the A domain, and the loop connecting the A domain and the C domain; and wherein the mutation alters the binding of starch to the variant alpha amylase polypeptide compared to the parental alpha amylase polypeptide.
Abstract translation: 描述了用于工业过程中的α-淀粉酶的变体，例如淀粉的液化。 α-淀粉酶变体具有增加的比活性，允许在液化过程期间峰值粘度的更快速降低。 通过在母体家族13α-淀粉酶多肽的氨基酸序列中引入淀粉结合槽中的氨基酸残基处的突变来修饰α-淀粉酶; 其中淀粉结合槽由A结构域中的第一个β链之前的α-螺旋中的氨基酸残基形成，A结构域中的第六个α-螺旋和第七个β链之间的环， A域中的第七个α-螺旋和第八个β链，以及连接A结构域和C结构域的环; 并且其中所述突变改变了与亲本α-淀粉酶多肽相比淀粉与变体α淀粉酶多肽的结合。
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公开(公告)号：WO2011063308A8
公开(公告)日：2011-05-26
申请号：PCT/US2010/057531
申请日：2010-11-19
Applicant: DANISCO US INC. , BOTT, Richard R. , KAPER, Thijs , KELEMEN, Bradley , GOEDEGEBUUR, Frits , HOMMES, Ronaldus Wilhelmus , KRALJ, Slavko , KRUITHOF, Paulien , NIKOLAEV, Igor , VAN DE KLEY, Pim , VAN LIESHOUT, Johan , VAN STIGT-THANS, Sander , VOGTENTANZ, Gudrun , SANDGREN, Mats
Inventor： BOTT, Richard R. , KAPER, Thijs , KELEMEN, Bradley , GOEDEGEBUUR, Frits , HOMMES, Ronaldus Wilhelmus , KRALJ, Slavko , KRUITHOF, Paulien , NIKOLAEV, Igor , VAN DE KLEY, Pim , VAN LIESHOUT, Johan , VAN STIGT-THANS, Sander , VOGTENTANZ, Gudrun , SANDGREN, Mats
IPC: C12N9/42
Abstract: The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
