公开(公告)号：WO2017031424A1

公开(公告)日：2017-02-23

申请号：PCT/US2016/047772

申请日：2016-08-19

Applicant: PEPSICO, INC.

Inventor： ANDERSON, Andrew ,  CHU, Alex ,  DING, Chris

IPC: A23L2/60 ,  A23L27/30 ,  C12P19/56

CPC classification number: A23L27/36 ,  C12P19/56

Abstract: This disclosure describes a biotechnological method for producing rebaudioside M (Reb M), using recombinantly produced glucosyltransferases, which can be carried out in a single reaction vessel using two glucosyltransferases and a uridine diphosphate glucose (UDPG) regenerating system. The method can be used to produce the commercially desirable Reb M from rubusoside, stevioside, or rebaudioside A (Reb A), which are all naturally more abundant steviol glycosides but with commercially less desirable properties.

Abstract translation: 本公开描述了使用重组产生的葡糖基转移酶来生产新蛇菊苷M（Reb M）的生物技术方法，其可以使用两个葡糖基转移酶和尿苷二磷酸葡萄糖（UDPG）再生系统在单个反应容器中进行。 该方法可用于从罗红糖苷，甜菊苷或新蛇菊苷A（Reb A）产生商业上期望的Reb M，其全部天然更丰富的甜菊糖苷，但具有商业上不理想的性质。