公开(公告)号：WO02054044A2

公开(公告)日：2002-07-11

申请号：PCT/US0150753

申请日：2001-12-28

Applicant: PICOLITER INC

Inventor： MUTZ MITCHELL W ,  ELLSON RICHARD N ,  LEE DAVID SOONG-HUA

IPC: G01N33/48 ,  B41J2/14 ,  C12M1/26 ,  C12M1/34 ,  C12M3/00 ,  C12N5/00 ,  C12Q1/02 ,  G01N1/28 ,  G01N15/10 ,  G01N15/14 ,  G01N30/02 ,  G01N37/00 ,  G01N15/00

CPC classification number: B41J2/14008 ,  C12M47/04 ,  G01N15/1056 ,  G01N15/1456 ,  G01N29/028 ,  G01N30/02 ,  G01N35/1074 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2035/1039 ,  B01D2015/389

Abstract: A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.

Abstract translation: 提供了一种用于从通道或其它容器声学喷射多个流体液滴的液滴，每个流体液滴包含一个或多个颗粒或其它局部容积。 可以是活细胞的局部体积被排出到基底表面，容器或通道上的位置。 还提供了一种综合的细胞分选和排列系统，其能够通过从载体液体中选择性地排出细胞而基于细胞特性进行分选。 可以以可调节的速度和轨迹弹出细胞。 排出的细胞可以被引导以形成阵列，其中阵列的每个位点可以包含单个细胞。 另外提供了一种形成单个活细胞阵列的方法，其比单体阵列方法更有效，快速，灵活和经济地形成。 该方法允许基于细胞性质的定量或半定量测量来对细胞进行有效，连续和同时的分选，并且还允许非二元或分支分支的决策。 还提供了一种包括处理器和方法的集成系统，用于从流体悬浮颗粒的连续流或在通道中流动的其它外接体积检测，选择和排出所选择的颗粒或外切体积（例如活细胞） 。

公开(公告)号：WO2004063029A2

公开(公告)日：2004-07-29

申请号：PCT/US2004000522

申请日：2004-01-09

Applicant: PICOLITER INC ,  MUTZ MITCHELL W ,  LEE DAVID SOONG-HUA ,  MCLENDON GEORGE

Inventor： MUTZ MITCHELL W ,  LEE DAVID SOONG-HUA ,  MCLENDON GEORGE

IPC: B41J2/14 ,  B65D

CPC classification number: B41J2/14008

Abstract: Devices and methods are provided for reducing uncontrolled electrostatic charge that can alter the volume and/or trajectory of a droplet, which may be ejected through the application of focused acoustic radiation. Also provided are reservoirs and substrates, e.g., well plates formed from a material that is at least partially nonmetallic or polymeric and either has an electrical resistivity of no more than about 10 ohm-cm, has a surface electrical resistivity of no more than about 10 ohm/sq, or both.

Abstract translation: 提供了用于减少不受控制的静电电荷的装置和方法，该静电电荷可以改变通过应用聚焦声辐射而喷射的液滴的体积和/或轨迹。 还提供了储存器和基板，例如由至少部分非金属或聚合物材料形成的并且具有不大于约10 11欧姆 - 厘米的电阻率的材料形成的孔板，其表面电阻率不再 大约10 12欧姆/平方英尺，或两者兼而有之。