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公开(公告)号：WO2020014190A1
公开(公告)日：2020-01-16
申请号：PCT/US2019/040945
申请日：2019-07-09
Applicant: SANDSTONE DIAGNOSTICS, INC.
Inventor： SCHAFF, Ulrich , HONG, Kyungjin , FREDRIKSEN, Laura , IACOVETTI, Gabriella , EPPERSON, Jon , RAHIMIAN, Ali , SOMMER, Greg , HONG, Sean , KENDALL, Eric
Abstract: A centrifugal cartridge or disk used for extraction of light supernatant fractions from fluid samples is described, particularly for non-homogenous fluid biological samples such as whole blood. The device may be used to collect cell-free blood plasma or a fraction of whole blood containing target cells such as leukocytes. Single or multiple channels are described, including channels with passive valves, gaskets, receiving cavities, inlet holes, capillary wicking ridges, distal cavities for cell retention, separator gel, and density medium. Centrifugal action causes whole blood in a receiving cavity to pass into one or more channels where it separates into blood cells, plasma and optionally fractions of an intermediate density. After spin, the plasma returns to the receiving cavity by way of the one or more channels for extraction through the inlet hole or other inwardly located hole. Disposable cartridges are constructed of monolithic top and bottom plates, which may be joined together by an elastomeric outer seal.
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公开(公告)号：WO2019240959A1
公开(公告)日：2019-12-19
申请号：PCT/US2019/034663
申请日：2019-05-30
Applicant: SANDSTONE DIAGNOSTICS, INC.
Inventor： SCHAFF, Ulrich , HONG, Kyungjin , FREDRIKSEN, Laura , EPPERSON, Jon
Abstract: A device and method for automated imaging of samples are described, particularly for non-homogeneous fluid biological samples. One purpose is particle quantification and other analysis. A device comprises a fluidic disk, a monolithic optical subsystem comprising a camera, imaging optics, rotational and focus motors, controller, and user interface. In a method, the fluidic disk receives a biological sample containing particles of interest and distributes them into multiple channels of defined thickness. Spatially distinct portions of the sample are drawn into each channel. The fluidic disk is then rotated by a motor such that a portion of each channel comes into alignment with the imaging optics and then each portion is imaged sequentially, thereby compensating for sample non-homogeneity. Embodiments include monochromatic LED illumination and image processing and particle counting in the channel images.
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公开(公告)号：WO2019215273A1
公开(公告)日：2019-11-14
申请号：PCT/EP2019/061902
申请日：2019-05-09
Applicant: F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE MOLECULAR SYSTEMS, INC. , SANDSTONE DIAGNOSTICS, INC.
Inventor： EPPERSON, Jon , FREDRIKSEN, Laura , HONG, Kyungjin , LI, Jenq-Thun , PARIKH, Bhairavi, Rajiv , SCHAFF, Ulrich
IPC: G01N33/543 , G01N33/569
Abstract: A cartridge for determining a concentration of target cells within a sample includes a separation portion and a detection portion. The separation portion includes a first and second surface defining a separation chamber. The separation portion can contain a density medium having a density greater than a density of a first portion of the sample and less than a density of a second portion of the sample (that includes the target cells). The separation chamber can be fluidically coupled to an inlet reservoir such that the sample can pass from the inlet reservoir to the separation chamber during rotation. The detection portion includes a detection surface that forms a boundary of a detection chamber. The detection surface is nonparallel to the first surface such that the target cells impinge on the detection surface when passing into the detection chamber. The detection surface is configured to capture the target cells.
