公开(公告)号：WO1993010260A1

公开(公告)日：1993-05-27

申请号：PCT/US1992010028

申请日：1992-11-18

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD ...

Inventor： THE BOARD OF TRUSTEES OF THE LELAND STANFORD ... ,  GOOCHEE, Charles, F. ,  GRAMER, Michael, J.

IPC: C12Q01/34

CPC classification number: C07K14/46 ,  C12N9/2402 ,  C12P19/14 ,  C12Y302/01018

Abstract: A neuraminidase enzyme with unusually high activity under extracellular cell-culture conditions has been discovered in Chinese hamster ovary (CHO) cells and other types of cells. Knowledge of the existence of this enzyme allows greater control over post-expression, extracellular oligosaccharide degradation than was previously possible in the absence of this discovery by using standard processing steps selected specifically to control the newly discovered activity and/or by using neuraminidase inhibitors.

Abstract translation: 已经在中国仓鼠卵巢（CHO）细胞和其他类型的细胞中发现了细胞外细胞培养条件下异常高活性的神经氨酸酶。 通过使用特别选择的标准处理步骤和/或使用神经氨酸酶抑制剂，知道这种酶的存在允许更好地控制后表达，细胞外寡糖降解，比先前在不存在该发现的情况下可能的那样。