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公开(公告)号：WO2018160677A1
公开(公告)日：2018-09-07
申请号：PCT/US2018/020193
申请日：2018-02-28
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： MORRISON, Richard H. , MAGYAR, Andrew P.
IPC: G01N27/414 , H01L29/78 , H01L29/66
CPC classification number: G01N27/4146 , G01N27/4145 , H01L29/0692 , H01L29/66795 , H01L29/7788 , H01L29/785 , H01L29/7855
Abstract: Aspects of the invention are directed to chemical and biological molecule sensing devices, methods of fabricating the chemical sensor devices, and methods of using those devices to detect chemical and biological molecules. The chemical sensor device may comprise a chemically-sensitive vertical slit field effect transistor (VeSFET) with a chemical recognition element attached to a gate structure and/or a channel of the VeSFET. The recognition element may be capable of binding to a chemical of interest such that the binding of the chemical to the recognition element results in a modification of current flow of the VeSFET, resulting in a detectable signal. The chemical sensor device may further comprise an amplifier configured to receive the detectable signal and produce an amplified signal, and an analog-to-digital converter (ADC) configured to receive the amplified signal and to produce a digital signal that represents the amplified signal.
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公开(公告)号：WO2019036335A1
公开(公告)日：2019-02-21
申请号：PCT/US2018/046441
申请日：2018-08-13
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： BARRON, Sara , GRAY HALEY, Caprice , KOPA, Anthony , MAGYAR, Andrew P.
Abstract: A wire includes an elongate conductive core wire, an elongate insulating layer disposed on and surrounding the elongate conductive core wire, an elongate conductive shield wire disposed adjacent to the insulating layer and the elongate conductive core wire, an elongate conductive shield layer disposed on the insulating layer and on the conductive shield wire such that the elongate insulating layer, the elongate conductive core wire, and the elongate conductive shield wire are embedded in the elongate conductive shield layer, the elongate conductive shield wire being electrically connected to the elongate conductive shield layer.
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公开(公告)号：WO2017222711A1
公开(公告)日：2017-12-28
申请号：PCT/US2017/033772
申请日：2017-05-22
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
IPC: C12Q1/68
Abstract: A composition for use in extending a molecular chain includes a carrier group and a payload group. The carrier group comprising a signaling group and a blocking group. A first bond bonds the payload group to the carrier group and a second bond bonds the signaling group to the blocking group. The first and second bonds are selectively cleavable such that the first bond is cleavable without cleaving the second bond. The payload group is to be attached to the molecular chain, while the blocking group is to block further attachments to the molecular chain once the payload group has been attached. The signaling group comprises a photon emitter that emits a signature photon in response to interrogation by an interrogatory photon. The carrier group transitions from a loaded state to an empty state upon cleavage of the first covalent bond.
Abstract translation: 用于扩展分子链的组合物包括载体组和有效负载组。 载体组包含信号传导组和阻断组。 第一键将有效载荷组键合到载体组，第二键将信号键键合到阻挡组。 第一和第二键可选择性切割，使得第一键可切割而不切割第二键。 有效载荷组将被连接到分子链上，而阻断基团则会在有效载荷组附着后阻断进一步的分子链附着。 信号灯组包括响应于询问光子的询问而发射签名光子的光子发射器。 在第一共价键断裂时，载体基团从负载状态转变为空状态。
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公开(公告)号：WO2019075321A1
公开(公告)日：2019-04-18
申请号：PCT/US2018/055600
申请日：2018-10-12
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
IPC: B01L3/00
CPC classification number: B01L3/50853 , B01L2200/0689 , B01L2300/041 , B01L2300/044 , B01L2300/0636 , B01L2300/0896
Abstract: Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.
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公开(公告)号：WO2019055990A1
公开(公告)日：2019-03-21
申请号：PCT/US2018/051526
申请日：2018-09-18
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： FRANK, Ian W. , MAGYAR, Andrew P.
CPC classification number: B01L3/502715 , B01J2219/00286 , B01J2219/00389 , B01J2219/00418 , B01J2219/00576 , B01J2219/00675 , B01J2219/00704 , B01J2219/00722 , B01L3/502761 , B01L2200/0663 , B01L2300/0654 , B01L2300/0877 , B82Y20/00 , G01N21/648 , G01N21/7746 , G01N2021/0346
Abstract: An apparatus includes a nanophotonic cavity, a waveguide, a microfluidic channel, and a photon detector. The cavity and the waveguide are fabricated out of a wide band gap semiconductor with an index of refraction that provides index contrast with the synthesis medium. One arrangement includes a photonic crystal having walls defining a channel for directing fluid flow. A functionalized spot is disposed in the channel for being tethered to a seed molecule to which a plurality of mers will be added. The walls extend downstream of the functionalized spot by a length that is at least as long as said molecule when assembled. The molecule can be linearized by a suitable fluid flow.
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公开(公告)号：WO2018217689A1
公开(公告)日：2018-11-29
申请号：PCT/US2018/033798
申请日：2018-05-22
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： MAGYAR, Andrew P. , CAVANAGH, Peter , ROGERS, Miles , MCFARLAND, Kirsty A. , SPRACHMAN, Melissa , BILLINGS-SIUTI, Amanda Nicole , RAUSTAD, Nicole E.
IPC: C12P19/34 , C12N9/12 , C07C245/08
CPC classification number: C12P19/34 , C07C245/08 , C07C247/04 , C12N9/1264
Abstract: Described herein are genetically engineered template-independent DNA polymerases, specifically terminal deoxynucleotidyl transferases, and methods of using these polymerases to control DNA synthesis by adding a single nucleotide (mononucleotide) at a time to the 3' end of a growing single-stranded DNA polynucleotide.
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公开(公告)号：WO2018129328A1
公开(公告)日：2018-07-12
申请号：PCT/US2018/012598
申请日：2018-01-05
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： MAGYAR, Andrew P. , SPRACHMAN, Melissa
IPC: C07F5/02 , C07K1/08 , C07K9/00 , C07F7/08 , C12Q1/6874
Abstract: Compositions and methods for optically-verified, sequence-controlled polymer synthesis are described.
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公开(公告)号：WO2019075211A1
公开(公告)日：2019-04-18
申请号：PCT/US2018/055432
申请日：2018-10-11
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： MAGYAR, Andrew P. , SPRACHMAN, Melissa M. , HUANG, Haiyao , HOEFLER, Brian , SWITKES, Michael
IPC: C12Q1/6806 , C12Q1/6825
CPC classification number: C12Q1/6806 , C12Q1/6825 , C12Q2521/10 , C12Q2525/186 , C12Q2533/10
Abstract: An oligonucleotide is prepared by the controlled, iterative addition of nucleotides to seed molecules at a functionalized electrode on a digital microfluidic device (DMF). Droplets containing a payload such as specific nucleotides and/or an enzyme, terminal deoxynucleotidyl transferase (TdT), for example, are individually guided, via path electrodes, to the functionalized electrodes, where chains are grown by addition of one nucleotide at a time. The DMF device includes an input section, containing reservoirs for reagents, a reaction section for growing the nucleotide chains and an output section for collecting waste and product. Each unit electrode on the device can be individually and independently addressed.
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公开(公告)号：WO2019068006A1
公开(公告)日：2019-04-04
申请号：PCT/US2018/053582
申请日：2018-09-28
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： MCFARLAND, Kirsty A. , MAGYAR, Andrew P. , PANG, Ting
IPC: C07K14/435 , C12N15/09
CPC classification number: C12N15/09 , C07K14/435
Abstract: Stimulus-controlled cell lysis compositions and methods of their use are described.
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公开(公告)号：WO2017222710A1
公开(公告)日：2017-12-28
申请号：PCT/US2017/033770
申请日：2017-05-22
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： FRANK, Ian W. , MAGYAR, Andrew P. , KORN, Jeffrey , PATEL, Neil S.
Abstract: An apparatus for optically-verified de novo DNA synthesis includes a microfluidic system that has channels leading in and out of a synthesis chamber having a functionalized region on a floor thereof on which a single-strand of DNA to which a nucleotide is to be attached can be fixed. The chamber is in optical communication with both an illumination system, which excites an electron in a fluorophore that is attached to the DNA strand, a detection system, which detects a signature photon emitted as the excited electron decays into its ground state.
Abstract translation: 用于光学验证的从头DNA合成的设备包括微流体系统，其具有通入和流出合成室的通道，所述合成室具有在其地板上的功能化区域，在所述合成室中单链DNA与 核苷酸将被连接的位置可以被固定。 该室与一个照射系统进行光通信，该系统激发附着在DNA链上的荧光团中的一个电子，一个检测系统，该系统检测随着被激发的电子衰变到其基态而发射的特征光子。
