公开(公告)号：WO2022187319A2

公开(公告)日：2022-09-09

申请号：PCT/US2022/018458

申请日：2022-03-02

Applicant: UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION, INC.

Inventor： CENTNER, Connor ,  KOPECHEK, Jonathan ,  BATES, Paula

IPC: B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  B01D21/28 ,  C12M47/04

Abstract: Devices and methods enable in-line separation/filtration of cells or particles during fill- and-finish operations without requiring external processing steps. Cell/particle separation and volume reduction is achieved using cationic microbubbles or microparticles in combination with acoustic radiation force via high pressure ultrasound waves, which deflect the cells or particles toward the wall to concentrate them into one side of the channel. The rate of cell/particle movement is directly dependent on their acoustic contrast, which is primarily a function of size, density, and compressibility. Therefore, cells or particles with different size, density, or compressibility can be separated with this technique.